The mammalian NudC-like genes: a family with functions other than regulating nuclear distribution

Riera, José; Lazo, Pedro Manuel Sánchez

doi:10.1007/s00018-009-0025-3

Cited by 25 publications

(24 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, microarray analysis showed that forced MYBL2 over expression transcriptionally activates a variety of genes involved in signal transduction and cell proliferation. Among these genes was MDK that activates the cell cycle as well as AKT and ERK1/2 pathways [26], RHO and RPS27 (MPS-1) that have been implicated in HCC progression [27,28], CSNK1D that regulates Wnt signaling [29], and NUDC involved in triggering the migration and proliferation of tumor cells [30]. Moreover, MYBL2 transfection led to the activation of HDAC10, whose inhibition has anti-proliferative and apoptogenic effects for different tumor types including HCC [31], as well as the activation of numerous ribosomal protein genes, representing the basis for the production of proteins driving cell growth.…”

Section: Discussionmentioning

confidence: 99%

Mybl2 expression is under genetic control and contributes to determine a hepatocellular carcinoma susceptible phenotype

Frau

Ladu

Calvisi

et al. 2011

Journal of Hepatology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Mybl2 expression is under genetic control and contributes to determine a hepatocellular carcinoma susceptible phenotype

Frau

Ladu

Calvisi

et al. 2011

Journal of Hepatology

View full text Add to dashboard Cite

“…For instance, inhibition of Hsp90 could directly inhibit cytoskeleton function, subsequently contributing to synthetic lethality when combined with a deletion in a vesicle trafficking gene product. In this regard, Hsp90’s interaction with the mammalian NudC protein is noteworthy [25, 64], because NudC is widely suspected to chaperone the assembly of dynein motor complexes [97–102]. Hsp90’s role in chaperoning myosin motors is similarly noteworthy [103–105].…”

Section: Discussionmentioning

confidence: 99%

Approaches for defining the Hsp90-dependent proteome

Hartson¹,

Matts²

2012

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

View full text Add to dashboard Cite

Hsp90 is the target of ongoing drug discovery studies seeking new compounds to treat cancer, neurodegenerative diseases, and protein folding disorders. To better understand Hsp90’s roles in cellular pathologies and in normal cells, numerous studies have utilized proteomics assays and related high-throughput tools to characterize its physical and functional protein partnerships. This review surveys these studies, and summarizes the strengths and limitations of the individual attacks. We also include downloadable spreadsheets compiling all of the Hsp90-interacting proteins identified in more than 23 studies. These tools include cross-references among gene aliases, human homologues of yeast Hsp90-interacting proteins, hyperlinks to database entries, summaries of canonical pathways that are enriched in the Hsp90 interactome, and additional bioinformatic annotations. In addition to summarizing Hsp90 proteomics studies performed to date and the insights they have provided, we identify gaps in our current understanding of Hsp90-mediated proteostasis.

show abstract

“…The Multiple Functions of NudC-NudC has been reported to play a role in mitosis and cytokinesis in various organisms from fungus to human (56). Deletion of Aspergillus NudC affects the composition and morphology of the cell wall and is lethal (57).…”

Section: Discussionmentioning

confidence: 99%

The L279P Mutation of Nuclear Distribution Gene C (NudC) Influences Its Chaperone Activity and Lissencephaly Protein 1 (LIS1) Stability

Zhu¹,

Liu²,

Jin

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

LIS1, a gene mutated in classical lissencephaly, plays essential roles in cytoplasmic dynein regulation, mitosis and cell migration. However, the regulation of LIS1 (lissencephaly protein 1) protein remains largely unknown. Genetic studies in Aspergillus nidulans have uncovered that the Nud (nuclear distribution) pathway is involved in the regulation of cytoplasmic dynein complex and a temperature-sensitive mutation in the nudC gene (L146P) greatly reduces the protein levels of NudF, an Aspergillus ortholog of LIS1. Here, we showed that L146 in Aspergillus NudC and its flanking region were highly conservative during evolution. The similar mutation in human NudC (L279P) obviously led to reduced LIS1 and cellular phenotypes similar to those of LIS1 down-regulation. To explore the underlying mechanism, we found that the p23 domain-containing protein NudC bound to the molecular chaperone Hsp90, which is also associated with LIS1. Inhibition of Hsp90 chaperone function by either geldanamycin or radicicol resulted in a decrease in LIS1 levels. Ectopic expression of Hsp90 partially reversed the degradation of LIS1 caused by overexpression of NudC-L279P. Furthermore, NudC was found to regulate the ATPase activity of Hsp90, which was repressed by the mutation of L279P. Interestingly, NudC itself was shown to possess a chaperone function, which also was suppressed by the L279P mutation. Together, these data suggest that NudC may be involved in the regulation of LIS1 stability by its chaperone function.

show abstract

The mammalian NudC-like genes: a family with functions other than regulating nuclear distribution

Cited by 25 publications

References 61 publications

Mybl2 expression is under genetic control and contributes to determine a hepatocellular carcinoma susceptible phenotype

Mybl2 expression is under genetic control and contributes to determine a hepatocellular carcinoma susceptible phenotype

Approaches for defining the Hsp90-dependent proteome

The L279P Mutation of Nuclear Distribution Gene C (NudC) Influences Its Chaperone Activity and Lissencephaly Protein 1 (LIS1) Stability

Contact Info

Product

Resources

About