The many faces of mismatch repair in meiosis

Borts, Rhona H.; Chambers, Scott R.; Abdullah, Mohamad Faiz Foong

doi:10.1016/s0027-5107(00)00044-0

Cited by 88 publications

(85 citation statements)

References 135 publications

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“…If the GC tract length is 100 bp or shorter, ;80% or more of the NCO recombination events would be undetectable; therefore, our results could suggest that GC tracts in Arabidopsis are very short. Alternatively, in a fraction of the DBS repair events, the repair of meiotic heteroduplex DNA could also result in restoration of the parental genotypes in regions flanking the initial DSBs, as observed in yeast (Borts et al 2000). A third possibility is that some DSBs might be repaired using the sister chromatids as templates, resulting in no GC.…”

Section: The Low Frequency Of Detected Ncos and Possible Short Gc Tractsmentioning

confidence: 99%

Analysis of Arabidopsis genome-wide variations before and after meiosis and meiotic recombination by resequencing Landsberg erecta and all four products of a single meiosis

et al. 2011

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Meiotic recombination, including crossovers (COs) and gene conversions (GCs), impacts natural variation and is an important evolutionary force. COs increase genetic diversity by redistributing existing variation, whereas GCs can alter allelic frequency. Here, we sequenced Arabidopsis Landsberg erecta (Ler) and two sets of all four meiotic products from a Columbia (Col)/Ler hybrid to investigate genome-wide variation and meiotic recombination at nucleotide resolution. Comparing Ler and Col sequences uncovered 349,171 Single Nucleotide Polymorphisms (SNPs), 58,085 small and 2315 large insertions/deletions (indels), with highly correlated genome-wide distributions of SNPs, and small indels. A total of 443 genes have at least 10 nonsynonymous substitutions in protein-coding regions, with enrichment for disease-resistance genes. Another 316 genes are affected by large indels, including 130 genes with complete deletion of coding regions in Ler. Using the Arabidopsis qrt1 mutant, two sets of four meiotic products were generated and analyzed by sequencing for meiotic recombination, representing the first tetrad analysis with whole-genome sequencing in a nonfungal species. We detected 18 COs, six of which had an associated GC event, and four GCs without COs (NCOs), and revealed that Arabidopsis GCs are likely fewer and with shorter tracts than those in yeast. Meiotic recombination and chromosome assortment events dramatically redistributed genome variation in meiotic products, contributing to population diversity. In particular, meiosis provides a rapid mechanism to generate copy-number variation (CNV) of sequences that have different chromosomal positions in Col and Ler.

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Section: The Low Frequency Of Detected Ncos and Possible Short Gc Tractsmentioning

confidence: 99%

Analysis of Arabidopsis genome-wide variations before and after meiosis and meiotic recombination by resequencing Landsberg erecta and all four products of a single meiosis

et al. 2011

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“…If these chromosomes are diverged, there will be mismatches in the heteroduplex DNA, which are detected by the mismatch repair system. Mismatches may be repaired, causing gene conversion, or recombination may be aborted, a phenomenon called antirecombination (Borts et al, 2000). In yeast, chromosomes must recombine with their homologues to assure proper meiotic segregation (Roeder, 1997).…”

Section: Antirecombinationmentioning

confidence: 99%

Reproductive isolation in Saccharomyces

2008

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Although speciation is one of the most interesting processes in evolution, the underlying causes of reproductive isolation are only partially understood in a few species. This review summarizes the results of many experiments on the reproductive isolation between yeast species of the Saccharomyces sensu stricto group. Hybrids between these species form quite readily in the laboratory, but, if given a choice of species to mate with, some are able to avoid hybridization. F1 hybrids are viable but sterile: the gametes they produce are inviable. For one pair of species, hybrid sterility is probably caused by chromosomal rearrangements, but for all the other species, the major cause of hybrid sterility is antirecombination-the inability of diverged chromosomes to form crossovers during F1 hybrid meiosis. Surprisingly, incompatibility between the genes expressed from different species' genomes is not a major cause of F1 hybrid sterility, although it may contribute to reproductive isolation at other stages of the yeast life cycle.

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“…In eukaryotic species, there are six MutS proteins (Msh1-6). Among them, Msh2, Msh3, and Msh6 have been shown to take part in DNA mismatch repair (MMR) (Borts et al 2000;Kolas and Cohen 2004), whereas only Msh4 and Msh5 are revealed to specifically get involved in the meiotic recombination process but not in MMR (Ross-Macdonald and Roeder 1994;Hollingsworth et al 1995;Zalevsky et al 1999;Kneitz et al 2000;Novak et al 2001;Higgins et al 2004;Hoffmann and Borts 2004;Kolas and Cohen 2004;Neyton et al 2004;Her et al 2007). In S. cerevisiae, mutations in Msh4 and Msh5 lead to the disruption of CO formation and the nondisjunction of homologous chromosomes (Ross-Macdonald and Roeder 1994;Hollingsworth et al 1995).…”

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confidence: 99%

Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5

et al. 2014

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MSH4 encodes a MutS protein that plays a specialized role in meiosis. In eukaryotic species, such as budding yeast, mice, Caenorhabditis elegans, and Arabidopsis, msh4 mutants display meiotic defects with a reduced number of chiasmata. Here, we characterized rice MSH4 by map-based cloning. In Osmsh4 mutants, the chiasma frequency was dramatically decreased to 10% of the wild type, but the synaptonemal complex was normally installed. The double mutant analysis showed that in the Osmsh4 Osmsh5 mutant, the reduction of chiasmata was greater than other zmm mutants. This was consistent with the absence of localization for OsZIP4 and OsMER3 in Osmsh4 and suggests an earlier role for OsMSH4 and OsMSH5 than other ZMM proteins where they may be required to stabilize progenitor Holliday junctions. Using yeast two-hybrid and pull-down assays, we verified the direct physical association between OsMSH4 and OsMSH5 and OsMSH5 and HEI10 in plants for the first time. The MSH4-MSH5 heterodimer has been demonstrated in mammals to stabilize the formation of progenitor and double Holliday junctions that may be resolved as crossovers (COs). We propose that OsMSH4 interacts with OsMSH5 to promote formation of the majority of COs in rice. MEIOSIS is a special form of cell division that generates haploid gametes for sexual propagation. Meiocytes undergo an intricate and elaborate process: one round of chromosome replication is followed by two rounds of cell division, the unique meiosis I and the mitosis-like meiosis II, generating four haploid gametes. The first division segregates homologous chromosomes, whereas the second one splits sister chromosomes, thereby halving the chromosome content from diploid to haploid. The first division has been recognized as the crucial stage of meiosis, in which prophase I is particularly important. Prophase I can be divided into five dissimilar but inseparable stages according to their different chromosome structures and behaviors: leptotene, zygotene, pachytene, diplotene, and diakinesis, during which homologous chromosomes pair, synapse, and recombine (Zickler and Kleckner 1999;Li and Ma 2006). Pairing, synapsis, and recombination are well linked in a carefully coordinated mechanism. Homologs search out and pair with each other, facilitating the initiation of recombination. Meanwhile, early stages of recombination are also used to promote global pairing and synapsis of homologs. Mature synaptonemal complexes (SCs) form as the homologous chromosomes approach each other at a distance of 100 nm and are disassembled during diplotene (Sym and Roeder 1994). After the disassembly of SCs, homolog pairs are held together only by crossovers (COs) to ensure their alignment on the metaphase I plate. COs also create physical connections between homologous chromosomes to guarantee their normal segregation to the opposite poles. In consequence, the absence of COs causes random segregation of homologs at anaphase I, leading to the formation of aneuploid dyads and a reduction of bivalents, followed by the s...

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The many faces of mismatch repair in meiosis

Cited by 88 publications

References 135 publications

Analysis of Arabidopsis genome-wide variations before and after meiosis and meiotic recombination by resequencing Landsberg erecta and all four products of a single meiosis

Analysis of Arabidopsis genome-wide variations before and after meiosis and meiotic recombination by resequencing Landsberg erecta and all four products of a single meiosis

Reproductive isolation in Saccharomyces

Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5

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