THE MATERNAL POUCH AND DEVELOPMENT IN THE MARSUPIAL FROG <i>GASTROTHECA RIOBAMBAE</i> (FOWLER)

DelPino, Eugenia M.; Galarza, Maria L.; Albuja, Carmen M. De; Humphries, Adam

doi:10.2307/1540381

Cited by 47 publications

(42 citation statements)

References 5 publications

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“…Amphibians, with more particularly X. laevis and X. tropicalis [100], but also other species [6,80,101] are useful models for studying apoptosis during embryonic development. For that, it was first necessary to give a table of development.…”

Section: Resultsmentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

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Amphibians and more particularly X. laevis are models often used for studying apoptosis during embryonic development. Using several methods, searchers determined the localization of programmed cell deaths (PCD). Several experimental methods also have been used to understand the regulatory mechanisms of apoptosis, throughout development, contributing to elucidate the general action of several genes and proteins. Apoptosis occurs very early, with a first program under control of maternal genes expressed before MBT, in order to eliminate damaged cells before gastrulation, and a second program at the onset of gastrulation. PCD is also observed during neurulation. Then, apoptotic cells are observed in amphibian organogenesis and metamorphosis. Results of these researches showed both importance of PCD for embryonic development, and the complexity of its regulation. Results obtained can be useful to understand others aspects of the importance of apoptosis, particularly pathological aspects.

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Section: Resultsmentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

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“…Studies in chick (Graham et al, 1993;Smith and Graham, 2001) and other amniotes (Knabe et al, 2004) show increased levels of cell death in specific rhombomeres, particularly r3 and r5. However, there is no apoptosis specific to r3 and r5 in Xenopus, zebrafish, and mouse (Schilling and Kimmel, 1994;Ellies et al, 1997;Hensey and Gautier, 1998;Del Pino and Medina, 1998;Kulesa et al, 2004), suggesting that cell death is not solely responsible for segregating the streams. Thus, in a prepattern-type model the neural tube is thought to endow destination instructions and control exit points such that there are few directional cues necessary to produce the cranial neural crest cell pattern.…”

Section: Introductionmentioning

confidence: 99%

In vivo evidence for short- and long-range cell communication in cranial neural crest cells

2004

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The proper assembly of craniofacial structures and the peripheral nervous system requires neural crest cells to emerge from the neural tube and navigate over long distances to the branchial arches. Cell and molecular studies have shed light on potential intrinsic and extrinsic cues, which, in combination,are thought to ensure the induction and specification of cranial neural crest cells. However, much less is known about how migrating neural crest cells interpret and integrate signals from the microenvironment and other neural crest cells to sort into and maintain the stereotypical pattern of three spatially segregated streams. Here, we explore the extent to which cranial neural crest cells use cell-to-cell and cell-environment interactions to pathfind. The cell membrane and cytoskeletal elements in chick premigratory neural crest cells were labeled in vivo. Three-dimensional reconstructions of migrating neural crest cells were then obtained using confocal static and time-lapse imaging. It was found that neural crest cells maintained nearly constant contact with other migrating neural crest cells, in addition to the microenvironment. Cells used lamellipodia or short, thin filopodia (1-2 μm wide) for local contacts (<20 μm). Non-local, long distance contact (up to 100 μm) was initiated by filopodia that extended and retracted, extended and tracked, or tethered two non-neighboring cells. Intriguingly, the cell-to-cell contacts often stimulated a cell to change direction in favor of a neighboring cell's trajectory. In summary, our results present in vivo evidence for local and long-range neural crest cell interactions, suggesting a possible role for these contacts in directional guidance.

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“…In our study, G. excubitor embryos apparently used their large, fused external gills to absorb dissolved nutrients that crossed egg membranes from the pouch lumen. Matrotrophy has been suspected in marsupial frogs owing to the highly vascularized external gills and pouch membrane [2,21], but previous studies found no evidence of embryonic mass change during development in Gastrotheca riobambae [4]. Thus, our study is the first, we believe, to directly demonstrate maternal nutrient transfer in a marsupial frog.…”

mentioning

confidence: 55%

“…A consequence of brooding embryos enclosed within the body is that parents must provide respiratory gas exchange, often via a close connection between highly vascularized parental surfaces and egg membranes or gill structures of larvae [2,4,5]. While this close connection can provide opportunities for maternal nutrient transfer (i.e.…”

Section: Introductionmentioning

confidence: 99%

Pouch brooding marsupial frogs transfer nutrients to developing embryos

Warne

Catenazzi

2016

Biol. Lett.

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Marsupial frogs have a unique reproductive mode in which females carry eggs enclosed in a sealed dorsal brood pouch. While most anurans are considered to be oviparous with lecithotrophic eggs, the extensively vascularized membrane of the brood pouch in marsupial frogs suggests potential opportunities for nutrient transfer. We tested for matrotrophy in the live-bearing Gastrotheca excubitor (Hemiphractidae), through feeding insects labelled with a 13 C-fatty acid and a 15 N-amino acid to brooding marsupial frogs. We observed significant increases of d C and d 15N in both maternal pouch tissues and embryos, suggesting nutrient transfer. Embryo dry mass also increased with developmental stage, providing further direct evidence for matrotrophy. These results suggest that in addition to gas exchange, the vascularized brood pouch membrane of G. excubitor also enables maternal nutrient transfer. This finding revealed a suspected but untested trait in the evolution of parental care in marsupial frogs, in contrast to previous work on Gastrotheca species that release tadpoles, and suggests greater complexity in reproductive and provisioning modes than previously thought.

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THE MATERNAL POUCH AND DEVELOPMENT IN THE MARSUPIAL FROG GASTROTHECA RIOBAMBAE (FOWLER)

Cited by 47 publications

References 5 publications

Apoptosis in amphibian development

Apoptosis in amphibian development

In vivo evidence for short- and long-range cell communication in cranial neural crest cells

Pouch brooding marsupial frogs transfer nutrients to developing embryos

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