The materno-placental microbiome of gravid beef cows under moderate feed intake restriction

Hummel, Gwendolynn L; Woodruff, Kelly L; Austin, Kathy J.; Knuth, Ryan M; Williams, Jordan D; Cunningham-Hollinger, Hannah C

doi:10.1093/tas/txab172

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…To our knowledge, the current study is the first research investigating the microbiota composition of the surgical site during elective CSs in bovine medicine. Thus far, the microbiota assessment in the field of bovine obstetrics and reproduction aimed to describe the bacterial population of a gravid and virgin uterus, foetal fluids, digestive and respiratory systems of the foetus and the relation between the cow and calf microbiota and its impact on the new-born’s health [ 21 , 22 , 23 , 24 , 25 , 40 ]. The results of these studies unveiled unknown information about the microbiota of several tissues and organs previously considered sterile.…”

Section: Discussionmentioning

confidence: 99%

“…Our study could be better designed if a negative control group was considered, especially to assess the absence of bacterial DNA of the used sampling material, even though it is supposed to be sterile [ 23 , 24 ]. However, several studies have not confirmed the sterility of the used material [ 40 , 56 , 61 , 62 ] without it interfering with the interpretation of their results. Moreover, if several samples had been taken at different sites before the uterus incision [ 22 , 23 , 24 , 25 ], such as the veterinary gloves, skin of cows, muscular wound and foetal fluids, this might have aided to the assessment of the origin of the bacteria found in the surgical site.…”

Section: Discussionmentioning

confidence: 99%

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Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Djebala

Coria

Munaut

et al. 2023

Veterinary Sciences

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This study aimed to describe the bacterial composition of the surgical site during elective caesarean sections (CSs) using the 16Sr DNA amplicon sequencing performed in parallel to bacterial culture. The study involved 13 Belgian blue cows of a previous dataset of 76 animals. Bacteriology was performed on swabs sampled from visceral and parietal peritoneum during the CS. Amplicon sequencing was performed in six samples chosen randomly among the swabs positive for bacteriology and seven among the culture-negative swabs. A total of 2542 bacterial operational taxonomic units belonging to 567 genera were identified. The most often identified genus and species were Mycoplasma (44%) and Mycoplasma wenyonii (36%), respectively. Results showed no difference in microbiota composition between the culture-positive and -negative samples. However, a difference was observed between the bacteriology and amplicon sequencing results. Indeed, seven out of nine cultured strains were not identified by amplicon sequencing in the samples in which they were cultured. In contrast to bacteriology, amplicon sequencing unveiled the presence of bacterial DNA in all elective CSs. The most identified DNA is most likely derived from the haematogenous spread of bacteria to the surgical site. Furthermore, the cultured bacteria were not the dominant species in the sample from which they were cultured.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Djebala

Coria

Munaut

et al. 2023

Veterinary Sciences

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Characterization of the equine placental microbial population in healthy pregnancies

et al. 2023

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Bovine reproductive tract and microbiome dynamics: current knowledge, challenges, and its potential to enhance fertility in dairy cows

Gupta,

Sarkar,

Pal

et al. 2024

Front. Microbiomes

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The cattle production system focuses on maintaining an animal-based food supply with a lower number of cattle. However, the fecundity of dairy cows has declined worldwide. The reproductive tract microbiome is one of the important factors which can influence bovine fecundity. Therefore, reproductive tract microbiomes have been explored during the estrus cycle, artificial insemination, gestation, and postpartum to establish a link between the micro-communities and reproductive performance. These investigations suggested that microbial dysbiosis in the reproductive tract may be associated with declined fertility. However, there is a scarcity of comprehensive investigations to understand microbial diversity, abundance, shift, and host-microbiome interplay for bovine infertility cases such as repeat breeding syndrome (RBS). This review summarizes the occurrence and persistence of microbial taxa to gain a better understanding of reproductive performance and its implications. Further, we also discuss the possibilities of microbiome manipulation strategies to enhance bovine fecundity.

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The materno-placental microbiome of gravid beef cows under moderate feed intake restriction

Cited by 3 publications

References 15 publications

Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Characterization of the equine placental microbial population in healthy pregnancies

Bovine reproductive tract and microbiome dynamics: current knowledge, challenges, and its potential to enhance fertility in dairy cows

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