The Matrilin-3 T298M mutation predisposes for post-traumatic osteoarthritis in a knock-in mouse model

Seifer, Patrick; Haÿ, Eric; Fleischhauer, Lutz; Heilig, Juliane; Bloch, Wilhelm; Sonntag, Stephan; Shmerling, D. H.; Clausen‐Schaumann, Hauke; Aszódi, Attila; Niehoff, Anja; Cohen‐Solal, Martine; Paulsson, Mats; Wagener, Raimund; Zaucke, Frank

doi:10.1016/j.joca.2020.09.008

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…Sections were washed with Tris-buffered saline and digested with 10 μg/ml proteinase K (Sigma–Aldrich) using proteinase K buffer (50 mM Tris–HCl, 1 mM EDTA, and pH 7.4) for 10 min at 50 °C. Mouse anti-THBS1 (1:100; 20 μg/ml, sc-59887; Santa Cruz Biotechnology) or rabbit anti-MATN1 (1:500; ( 46 )) or rabbit anti-S100A9 (1:500; PA1-46489; Thermo Fisher Scientific) were first incubated at 4 °C overnight and then with corresponding Cy3-coupled secondary antibodies (1:800; Jackson ImmunoResearch) and 4′,6-diamidino-2-phenylindole (Sigma–Aldrich) for 1 h at RT. Sections were embedded in Mowiol and analyzed in a blinded manner using Nikon Eclipse TE2000-U microscope (Nikon).…”

Section: Methodsmentioning

confidence: 99%

“…The nitrocellulose membrane was incubated with 5% milk powder in TBS containing 0.1% Tween-20 for 1 h at RT. Primary antibody against THBS1 (1:1000; 20 μg/ml, sc-59887; Santa Cruz Biotechnology), MATN1 (1:500; ( 46 )), ACAN (1:500, AB1031; Merck Millipore), COL2 (1:500, CP18; Merck Millipore), VEGF-A (1:1000, sc-7269; Santa Cruz Biotechnology), and a-ACTA1 (1:200; 1 mg/ml; Merck Millipore) and appropriate horseradish peroxidase–labeled secondary antibodies (DAKO) were applied. Chemoluminescence reaction and X-ray films (Agfa Healthcare) were used to visualize specific binding.…”

Section: Methodsmentioning

confidence: 99%

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Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage

Bubb

Holzer

Nolte

et al. 2021

Journal of Biological Chemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage

Bubb

Holzer

Nolte

et al. 2021

Journal of Biological Chemistry

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“…The cartilage of the mutant mice contained collagen fibrils with increased diameter compared to wildtype, increasing the compressive stiffness of the tissue. Despite this altered cartilage property, the mutant mice showed no increase in spontaneous OA severity with age but did for surgically-induced (meniscectomy) post-traumatic OA, suggesting the matrillin-3 T298M knock-in mouse could represent a model for investigating the pathogenic mechanisms involved in OA development 20 .…”

Section: Functional Genetic Analysismentioning

confidence: 97%

Osteoarthritis year in review: genetics, genomics, epigenetics

Young

Barter

Soul

2022

Osteoarthritis and Cartilage

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Summary Objective In this review, we have highlighted the advances over the past year in genetics, genomics and epigenetics in the ﬁeld of osteoarthritis (OA). Methods A literature search of PubMed was performed using the criteria: “osteoarthritis” and one of the following terms “genetic(s), genomic(s), epigenetic(s), polymorphism, noncoding ribonucleic acid (RNA), microRNA, long noncoding RNA, lncRNA, circular RNA, RNA sequencing (RNA-seq), single cell sequencing, transcriptomics, or deoxyribonucleic acid (DNA) methylation between April 01, 2020 and April 30, 2021. Results In total we identiﬁed 765 unique publications, which eventually reduced to 380 of relevance to the field as judged by two assessors. Many of these studies included multiple search terms. We summarised advances relating to genetics, functional genetics, genomics and epigenetics, focusing on our personal key papers during the year. Conclusions This year few studies have identiﬁed new genetic variants contributing to OA susceptibility, but a focus has been on refining risk loci or their functional validation. The use of new technologies together with investigating the cross-talk between multiple tissue types, greater sample sizes and/or better patient classification (OA subtypes) will continue to increase our knowledge of disease mechanisms and progress towards understanding and treating OA.

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Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Hartmann,

Fleischhauer,

Nicolau

et al. 2024

Nat Protoc

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The Matrilin-3 T298M mutation predisposes for post-traumatic osteoarthritis in a knock-in mouse model

Cited by 4 publications

References 39 publications

Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage

Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage

Osteoarthritis year in review: genetics, genomics, epigenetics

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

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