The MdAP2-34 modulates flavonoid accumulation in apple (Malus domestica Borkh.) by regulating MdF3′H

Han, Dong; Huang, Benchang; Li, Yuchen; Dang, Qingyuan; Fan, Lianmei; Nie, Jiyun; Wang, Yongzhang; Yang, Yuan; Jia, Dongjie

doi:10.1016/j.postharvbio.2022.111994

Cited by 15 publications

(6 citation statements)

References 43 publications

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“…AP2/ERF also functions in the flavonoid accumulation, for example, CitERF32 and CitERF33 positively activate the transcription and enhance the flavonoid biosynthesis in the mandarin cultivar Ougan 55 . MdAP2-34 can modulate flavonoid accumulation by enhancing MdF3’H promoter activity in apple 56 . Similarly, a bZIP gene has been proven to regulate flavonoid biosynthesis in grape 57 .…”

Section: Discussionmentioning

confidence: 99%

An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

Wen,

Wang,

et al. 2024

Nat Commun

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Citrus reticulata cv. Chachiensis (CRC) is an important medicinal plant, its dried mature peels named “Guangchenpi”, has been used as a traditional Chinese medicine to treat cough, indigestion, and lung diseases for several hundred years. However, the biosynthesis of the crucial natural products polymethoxylated flavonoids (PMFs) in CRC remains unclear. Here, we report a chromosome-scale genome assembly of CRC with the size of 314.96 Mb and a contig N50 of 16.22 Mb. Using multi-omics resources, we discover a putative caffeic acid O-methyltransferase (CcOMT1) that can transfer a methyl group to the 3-hydroxyl of natsudaidain to form 3,5,6,7,8,3’,4’-heptamethoxyflavone (HPMF). Based on transient overexpression and virus-induced gene silencing experiments, we propose that CcOMT1 is a candidate enzyme in HPMF biosynthesis. In addition, a potential gene regulatory network associated with PMF biosynthesis is identified. This study provides insights into PMF biosynthesis and may assist future research on mining genes for the biosynthesis of plant-based medicines.

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Section: Discussionmentioning

confidence: 99%

An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

Wen,

Wang,

et al. 2024

Nat Commun

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“…The AP2/ERF superfamily of TFs regulates flavonoid biosynthesis by modulating genes responsible for metabolite biosynthesis [ 30 ]. For instance, the MdAP2-34 was found to promote flavonoid accumulation by directly binding to and activating the MdF3′H promoter in apples, thus promoting the flavonoid biosynthetic pathway [ 31 ]. Similarly, melatonin promotes the accumulation of flavonoids by suppressing the inhibition of BrERF2/BrERF109 on the transcripts of BrFLS1 and BrFLS3.2 , ultimately postponing leaf senescence in postharvest flowering Chinese cabbage [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Chrysanthemum (Chrysanthemum morifolium) CmHRE2-like negatively regulates the resistance of chrysanthemum to the aphid (Macrosiphoniella sanborni)

Wang,

Zhang,

Hong

et al. 2024

BMC Plant Biol

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Background The growth and ornamental value of chrysanthemums are frequently hindered by aphid attacks. The ethylene-responsive factor (ERF) gene family is pivotal in responding to biotic stress, including insect stress. However, to date, little is known regarding the involvement of ERF transcription factors (TFs) in the response of chrysanthemum to aphids. Results In the present study, CmHRE2-like from chrysanthemum (Chrysanthemum morifolium), a transcription activator that localizes mainly to the nucleus, was cloned. Expression is induced by aphid infestation. Overexpression of CmHRE2-like in chrysanthemum mediated its susceptibility to aphids, whereas CmHRE2-like-SRDX dominant repressor transgenic plants enhanced the resistance of chrysanthemum to aphids, suggesting that CmHRE2-like contributes to the susceptibility of chrysanthemum to aphids. The flavonoids in CmHRE2-like-overexpression plants were decreased by 29% and 28% in two different lines, whereas they were increased by 42% and 29% in CmHRE2-like-SRDX dominant repressor transgenic plants. The expression of Chrysanthemum-chalcone-synthase gene(CmCHS), chalcone isomerase gene (CmCHI), and flavonoid 3′-hydroxylase gene(CmF3’H) was downregulated in CmHRE2-like overexpression plants and upregulated in CmHRE2-like-SRDX dominant repressor transgenic plants, suggesting that CmHRE2-like regulates the resistance of chrysanthemum to aphids partially through the regulation of flavonoid biosynthesis. Conclusion CmHRE2-like was a key gene regulating the vulnerability of chrysanthemum to aphids. This study offers fresh perspectives on the molecular mechanisms of chrysanthemum-aphid interactions and may bear practical significance for developing new strategies to manage aphid infestation in chrysanthemums.

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“…SlERF.G3-like appeared to act independently of the flavonol-specific activator SlMYB12 [ 89 ]. Overexpressing MdAP2-34 in apple callus induced flavonol accumulation by targeting and activating the MdF3′H promoter [ 90 ]. Overexpression of citrus CsERF003 in tomato led to accumulation of flavonol glycosides and naringenin chalcone by activating expression of SlPAL , SlC4H , Sl4CL , SlCHS , SlCHI , SlF3′H , and SlFLS [ 91 ].…”

Section: Direct Transcriptional Regulation Of Flavonol Biosynthesismentioning

confidence: 99%

Transcriptional regulation of flavonol biosynthesis in plants

Cao,

Mei,

Zhang

et al. 2024

Horticulture Research

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Summary Flavonols are a class of flavonoids that play a crucial role in regulating plant growth and promoting stress resistance. They are also important dietary components in horticultural crops due to their benefits for human health. In past decades, research on the transcriptional regulation of flavonol biosynthesis in plants has increased rapidly. This review summarized recent progress in the flavonol-specific transcriptional regulation in plants, encompassing characterization of different categories of transcription factors (TFs) and microRNAs as well as elucidation of different transcriptional mechanisms including direct and cascade transcriptional regulation. The direct transcriptional regulation consists of TFs, such as MYB, AP2/ERF, WRKY, which can directly target the key flavonol synthase gene or other early genes in the flavonoid biosynthesis. In addition, different regulation modules in the cascade transcriptional regulation involve microRNAs targeting TFs, regulation between activators, interaction between activators and repressors, and degradation of activators or repressors induced by UV-B light or plant hormones. Such sophisticated regulation of the flavonol biosynthetic pathway in response to UV-B radiation or hormones may allow plants to fine-tune flavonol homeostasis, thereby balances plant growth and stress responses in a timely manner. Based on the orchestrated regulation, molecular design strategies will be applied to breed horticultural crops with excellent health-promoting effects and high resistance.

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The MdAP2-34 modulates flavonoid accumulation in apple (Malus domestica Borkh.) by regulating MdF3′H

Cited by 15 publications

References 43 publications

An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

Chrysanthemum (Chrysanthemum morifolium) CmHRE2-like negatively regulates the resistance of chrysanthemum to the aphid (Macrosiphoniella sanborni)

Transcriptional regulation of flavonol biosynthesis in plants

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