The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit

Hayee, Bu; Sun, Xiaohuan; Yue, Pengtao; Qiao, Junling; Sun, Jiamao; Wang, Aide; Yuan, Hui; Wu, Yu

doi:10.3390/ijms23169454

Cited by 10 publications

(4 citation statements)

References 33 publications

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“…Phytohormones have been reported to affect organ size by altering the cell number or cell size [9]. For instance, a new study finds that the expression of an auxin signaling gene MdAux/IAA2 is repressed, leading to an increase in cell size and fruit weight of apple [66]. This finding is inconsistent with our results, which show that the expressions of five AUX/IAAs (PaF106G0100000592.0, PaF106G0300013748.01, PaF106G0300013749.01, PaF106G0800029753.01, and PaF106G0800029755.01) are significantly upregulated during the second cell expansion process (stages AF7 and AF8) in our larger-fruit 'Sungold' compared to small-fruit 'F43' (Figure 4b).…”

Section: Discussionmentioning

confidence: 99%

Comparative Anatomical and Transcriptomics Reveal the Larger Cell Size as a Major Contributor to Larger Fruit Size in Apricot

Huang

Zhu

Bai

et al. 2023

IJMS

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Fruit size is one of the essential quality traits and influences the economic value of apricots. To explore the underlying mechanisms of the formation of differences in fruit size in apricots, we performed a comparative analysis of anatomical and transcriptomics dynamics during fruit growth and development in two apricot cultivars with contrasting fruit sizes (large-fruit Prunus armeniaca ‘Sungold’ and small-fruit P. sibirica ‘F43’). Our analysis identified that the difference in fruit size was mainly caused by the difference in cell size between the two apricot cultivars. Compared with ‘F43’, the transcriptional programs exhibited significant differences in ‘Sungold’, mainly in the cell expansion period. After analysis, key differentially expressed genes (DEGs) most likely to influence cell size were screened out, including genes involved in auxin signal transduction and cell wall loosening mechanisms. Furthermore, weighted gene co-expression network analysis (WGCNA) revealed that PRE6/bHLH was identified as a hub gene, which interacted with 1 TIR1, 3 AUX/IAAs, 4 SAURs, 3 EXPs, and 1 CEL. Hence, a total of 13 key candidate genes were identified as positive regulators of fruit size in apricots. The results provide new insights into the molecular basis of fruit size control and lay a foundation for future breeding and cultivation of larger fruits in apricot.

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Section: Discussionmentioning

confidence: 99%

Comparative Anatomical and Transcriptomics Reveal the Larger Cell Size as a Major Contributor to Larger Fruit Size in Apricot

Huang

Zhu

Bai

et al. 2023

IJMS

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“…Plant hormones directly regulate the fruit size and shape growth and development by altering the expression of early response genes in horticultural plants. The high auxin content depressed the expression of MdAux/IAA2, and the downregulated expression of MdAux/IAA2 led to the formation of a large fruit size apple [22]. An important role for auxin in the regulation of fruit development, especially at the fruit enlargement stage, and three single nucleotide polymorphism (SNP) markers were also closely associated with fruit weight in loquat [20].…”

Section: In Silico Prediction Of the Putative Functions Of The Candid...mentioning

confidence: 99%

“…Despite the importance of fruit size, its underlying molecular mechanisms remain understudied in jujube. Studies have shown that fruit size traits are controlled by polygenes with weak inheritability [21] and are directly or indirectly regulated by one or more hormones [22,23].…”

Section: Introductionmentioning

confidence: 99%

Construction of a High-Density Genetic Linkage Map Based on Bin Markers and Mapping of QTLs Associated with Fruit Size in Jujube (Ziziphus jujuba Mill.)

et al. 2023

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Jujube (Ziziphus jujuba Mill.) is a fruit tree that is gaining increasing importance in drought-affected regions worldwide. The fruit size is an important quantitative agronomic trait that affects not only the fruit yield and attractiveness but also consumer preference. Genetic enhancement of fruit appearance is a fundamental goal of jujube breeding programs. The genetic control of jujube fruit size traits is highly quantitative, and development of high-density genetic maps can facilitate fine mapping of quantitative trait loci (QTLs) and gene identification. However, studies regarding the construction of high-density molecular linkage maps and identification of quantitative trait loci (QTLs) targeting fruit size in jujube are limited. In this study, we performed whole-genome resequencing of the jujube cultivars “JMS2” and “Xing16” and their 165 F1 progenies to identify genome-wide single-nucleotide polymorphism (SNP) markers and constructed a high-density bin map of jujube that can be used to assist in the selection of multiple traits in jujube breeding. This analysis yielded a total of 116,312 SNPs and a genetic bin map of 2398 bin markers spanning 1074.33 cM with an average adjacent interval of 0.45 cM. A quantitative genetic analysis identified 15 QTLs related to fruit size and the observed phenotypic variation associated with a single QTL ranged from 9.5 to 13.3%. Through the screening of overlapping and stable QTL regions, we identified 113 candidate genes related to fruit size. These genes were ascertained to be involved in cell division, cell wall metabolism, synthesis of phytohormones (ABA, IAA, and auxin), and encoding of enzymes and transcription factors. These candidate genomic regions will facilitate marker-assisted breeding of fruits with different sizes and shapes and lay a foundation for future breeding and manipulation of fruit size and shape in jujube.

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“…When TaIAA15 was ectopically expressed in rice, plant height and leaf angle were reduced [33]. In apple, MdIAA2 overexpression led to the formation of smaller fruit [34]. The overexpression of PpIAA19 (from peach (Prunus persica)) in tomato altered the plant development and fruit shape and substantially lengthened the internodes [35].…”

Section: Introductionmentioning

confidence: 99%

A New Function of MbIAA19 Identified to Modulate Malus Plants Dwarfing Growth

Wang,

Xue,

Zhang

et al. 2023

Plants

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The primary determinants of apple (Malus) tree architecture include plant height and internode length, which are the significant criteria for evaluating apple dwarf rootstocks. Plant height and internode length are predominantly governed by phytohormones. In this study, we aimed to assess the mechanisms underlying dwarfism in a mutant of Malus baccata. M. baccata dwarf mutant (Dwf) was previously obtained through natural mutation. It has considerably reduced plant height and internode length. A comparative transcriptome analysis of wild-type (WT) and Dwf mutant was performed to identify and annotate the differentially expressed genes responsible for the Dwf phenotype using RNA-seq and GO and KEGG pathway enrichment analyses. Multiple DEGs involved in hormone signaling pathways, particularly auxin signaling pathways, were identified. Moreover, the levels of endogenous indole-3-acetic acid (IAA) were lower in Dwf mutant than in WT. The Aux/IAA transcription factor gene MbIAA19 was downregulated in Dwf mutant due to a single nucleotide sequence change in its promoter. Genetic transformation assay demonstrated strong association between MbIAA19 and the dwarf phenotype. RNAi-IAA19 lines clearly exhibited reduced plant height, internode length, and endogenous IAA levels. Our study revealed that MbIAA19 plays a role in the regulation of dwarfism and endogenous IAA levels in M. baccata.

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The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit

Cited by 10 publications

References 33 publications

Comparative Anatomical and Transcriptomics Reveal the Larger Cell Size as a Major Contributor to Larger Fruit Size in Apricot

Comparative Anatomical and Transcriptomics Reveal the Larger Cell Size as a Major Contributor to Larger Fruit Size in Apricot

Construction of a High-Density Genetic Linkage Map Based on Bin Markers and Mapping of QTLs Associated with Fruit Size in Jujube (Ziziphus jujuba Mill.)

A New Function of MbIAA19 Identified to Modulate Malus Plants Dwarfing Growth

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