The Measurement of 11ß, 17ß-Dihydroxy-4-androsten-3-one (11ß-Hydroxytestosterone) by Radioimmunoassay in Human Plasma

Abstract

Summary: A radioimmunoassay (RIA) is described for the measurement of llß,17ß-dihydroxy-4-androsten-3-one (llß-hydroxytestosterone) in human plasma. The reliability criteria of the new RIA were similar to those of other steroid hormone radioimmunoassays. The mean plasma llß,17ß-dihydroxy-4-androsten-3-one level for healthy young subjects was 1.31 ± 0.32 nmol/1 (x ± SD) in males and 1.19 ± 0.35 nmol/1 in females at 8 a. m.; during the night, there was a marked decrease, and at 11 p. m. the recorded values were 0.41 ± 0.15 nmol/1 and 0.46 ± 0.14 nmol/1, respectively. During the corticotropin Stimulation test, llß,17ß-dihydroxy-4-androsten-3-one increased from 1.03 ± 0.33 nmol/1 to 1.31 ± 0.41 nmol/1, while in dexamethasone suppression tests a decrease from 2.04 ± 0.82 nmol/1 to 0.25 ± 0.05 nmol/1 was seen. In contrast, | chorionic gonadotropin administration on 3 consecutive days did not influence plasma concentrations of f 11 ß,17ß-dihydroxy-4-androsten-3-one. Radioimmunoassay zur Messung von llß f i7ß-Dihydroxy-4-androsten-3-on (llß-Hydroxytestosteron) im Plasma des MenschenZusammenfassung: Es wird ein Radioimmunoassay für die Messung von llß,17ß-Dihydroxy-4-androsten-3-on (llß-Hydroxytestosteron) im menschlichen Plasma beschrieben. Die Zuverlässigkeitskriterien des neu aufgebauten RIA entsprechen denen anderer Radioimmunoassays für Steroidhormone.Die Konzentration von llß,17ß-Dihydroxy-4-androsten-3-on betrug um 8.00 Uhr im Plasma gesunder Männer 1,31 + 0,32 nmol/1 ( ± SD) und bei gesunden Frauen 1,19 ± 0,35 nmol/1. Um 23.00 Uhr fanden ; sich 0,41 ± 0,15 nmol/1 (Männer) und 0,46 ± 0,14 nmol/1 (Frauen). Im Cörticotropin-Stimulationstest war ein Anstieg der Konzentration von llß,17ß-Dihydroxy-4-androsten-3-on im Plasma von 1,03 ± 0,33 nmol/1 | auf 1,31 ± 0,41 nmol/1 zu beobachten, während sich im Dexamethasontest ein Rückgang von 2,04 ± 0,82 ! nmol/1 auf 0,25 ± 0,05 nmol/1 ergab. Nach Applikation von Choriongonadotropin wurde keine Änderung i der Konzentration von llß,17ß-Dihydroxy-4-androsten-3-on gemessen. IntroductionRecently 17ß-hydroxy-4-androstene-3,ll-dione and The adrenal atidrogens are quantitatively the most 4-androstene-3, 11,17-trione were demonstrated in important steroid hormones but their physiological human plasma (2, 3). In contrast, no Information role reniains still somewhat obscure in man. Far less is ayailable about llß,17ß-dihydroxy-4-androsten-3-information is available about the adrenal androgens one in human peripheral blood. The present paper that carry a functional group at C-ll. llß-Hydroxy-describes a radioimmunoassay system for the meas-4-androstene-3,17-dione has been demonstrated in urement of llß,17ß-dihydroxy-4-androsten-3-one, human plasma and might play an important role and presents the fürst published values for this androunder physiological and pathological conditions (1). gen in human plasma.

The steroid metabolome of adrenarche

“…These C 19 steroids are also produced in small quantities by mouse gonads (109). The physiologic role of these steroids in humans has not been studied but circulating levels of these androgens have been examined in human blood (110, 111). 11OHT could be speculated to be an adrenal-derived androgen since it requires the activity of 11β-hydroxylation via the adrenal-specific enzyme CYP11B1 (110, 111).…”

“…The physiologic role of these steroids in humans has not been studied but circulating levels of these androgens have been examined in human blood (110, 111). 11OHT could be speculated to be an adrenal-derived androgen since it requires the activity of 11β-hydroxylation via the adrenal-specific enzyme CYP11B1 (110, 111). Because the adrenal produces large amounts of DHEA, DHEAS, androstenedione and 11OHA, it is likely that these androgen precursors will feed into both the classical and alternative peripheral metabolic pathways that can lead to active androgens.…”