2012
DOI: 10.5455/jib.20120813053832
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The Measurement of Asymmetric Dimethylarginine in Human Plasma by High Performance Liquid Chromatography

Abstract: Objective: We aimed to establish a high performance liquid chromatography (HPLC) method to measure plasma ADMA levels easily, sensitively and reliably. Methods: We used fluorescence detection, 50 mM sodium acetate buffer/ methanol/ Tetrahydrofuran as a mobile phase (A, 82:17:1; B, 22:77:1, % v, respectively), gradient method, 338 and 425 nm excitation and emission wavelengths, respectively. Samples were derivatizated with o-Phthaldialdehyde. Results: The recovery was found as 96 to 98,3 %. Intra-assay and inte… Show more

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“…Oxidative stress increases the activity of PRMTs and inhibits the DDAH, leading to increased levels of ADMA. 12 Increased levels of ADMA have recently been implicated in endothelial vasodilator dysfunction and coronary and arterial diseases, 46,47 which could be explained by ADMA inhibition of nitric oxide synthase leading to decreased nitric oxide. 48 Nitric oxide is a potent vasodilator and its altered biosynthesis has been implicated in the pathogenesis of cardiovascular diseases.…”
Section: Discussionmentioning
confidence: 99%
“…Oxidative stress increases the activity of PRMTs and inhibits the DDAH, leading to increased levels of ADMA. 12 Increased levels of ADMA have recently been implicated in endothelial vasodilator dysfunction and coronary and arterial diseases, 46,47 which could be explained by ADMA inhibition of nitric oxide synthase leading to decreased nitric oxide. 48 Nitric oxide is a potent vasodilator and its altered biosynthesis has been implicated in the pathogenesis of cardiovascular diseases.…”
Section: Discussionmentioning
confidence: 99%