The measurement of fungal growth in solid substrates

Matcham, S. E.; Wood, David А.; Jordan, Bill

doi:10.1007/bf02798989

Cited by 15 publications

(11 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, separation of mycelium from insoluble substrates is impracticable, which precludes comparisons of mycelial dry weight. The assay methods commonly used for measuring fungal biomass on solid substrates are chitin and ergosterol estimation (Matcham et al, 1984). Chitin is also an integral part of insect cuticle structure and thus ergosterol content was considered as a means of estimating biomass in this study.…”

Section: Ergosterol As a Measure Of Mycelial Biomass In M Anisopliaementioning

confidence: 99%

Specific induction of a cuticle-degrading protease of the insect pathogenic fungus Metarhizium anisopliae

et al. 1994

View full text Add to dashboard Cite

~The insect pathogenic fungus Metarhizium anisopliae produces several extracellular cuticle-degrading proteases and evidence is consistent that one of these, a chymoelastase PRl, is a determinant of pathogenicity. We have shown previously that the wide-domain regulatory circuits of carbon and nitrogen derepression regulate PRI production. In the present work we have established in addition that PRI is specifically induced by insect cuticle, but not by other soluble or insoluble proteinaceous substrates. The feeding of elastin or collagen to derepressed established mycelium (starved for carbon and nitrogen) did not enhance PR1 production significantly and the soluble proteins BSA and gelatin rapidly and completely repressed PRI. The carbohydrate polymers cellulose and xylan gave derepressed basal levels of PR1. However, addition of locust cuticle enhanced PRI production to a level approximately 10-fold that of derepressed mycelium. In order to establish if the enhancing effect of insect cuticle on PRI production was due to specific induction or merely a reflection of enhanced growth on this insoluble dual carbon and nitrogen source, ergosterol was used as a measure of fungal growth. Expressing enzyme activity per mg dry weight showed that PRI production in cuticle cultures increased approximately five-and ninefold after 12 and 24 h growth compared with elastin-grown cultures. Thus, the substantial increase in PR1 production on cuticle was shown not to be a function of fungal growth and this confirms that PRI is induced by a component of insect cuticle; we believe this is the first report of induction by a specific substrate for any microbial protease.

show abstract

Section: Ergosterol As a Measure Of Mycelial Biomass In M Anisopliaementioning

confidence: 99%

Specific induction of a cuticle-degrading protease of the insect pathogenic fungus Metarhizium anisopliae

et al. 1994

View full text Add to dashboard Cite

show abstract

“…All other methods available up to now are much more time-consuming. Nevertheless, they do not measure biomass directly but either a selected activity or compound of the hyphae and rely on the premise that the activity or content of these compounds per biomass is independent from growth conditions or culture age (MATCHAM et al 1984, BERMINGHAM et al 1995. Our measurement of substrate decomposition (loss of organic matter) showed that the D. squalens studied was growing best at temperatures above 30 °C.…”

Section: Discussionmentioning

confidence: 97%

Influence of incubation temperature on activity of ligninolytic enzymes in sterile soil byPleurotus sp. andDichomitus squalens

Lang¹,

Gonser²,

Zadražil³

2000

J. Basic Microbiol.

View full text Add to dashboard Cite

“…Such methods have been reviewed (Matcham et at., 1984). Nevertheless, substrate components could interfere, and such measurements do not distinguish between dead and live mycelium.…”

Section: Biomassmentioning

confidence: 98%