The measurement of luminescence waveforms by single-photon techniques

Cova, S.; Bertolaccini, M.; Bussolati, C.

doi:10.1002/pssa.2210180102

Cited by 49 publications

(19 citation statements)

References 33 publications

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“…I n fact, the differences that can be observed between the interpolating z( 2') curves corresponding t o different models proposed in the literature [3, 41 are rather small. The niost precise experimental data available in the literature have therefore been considered here and, for some crystals, measurements have been performed by using the single photon delay distribution (SPDD) measureiiient technique [ 7 ] . The lifetimes t ( T ) thus measured as a function of temperature are shown in Fig.…”

Section: Experimental Data and Discussionmentioning

confidence: 99%

A contribution to the discussion on the relaxed excited F centre

Bosi¹,

Cova²,

Spinolo³

1975

Physica Status Solidi (b)

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An interpretation is given of the behaviour of the relaxed excited F centre in ternis of a hydrogenic model with broad wave functions. I n particular the dependence is considered of the lifetime T on the host crystalline matrix a t a fixed low temperature, and on the temperature T for a given matrix. Precision experimental data are given for t ( T ) in KaCI, RbCl, and KBr.On discute l'interpr6tation du comportement de 1'6tat excite relax6 du centre F par nn model hydrogenique avec des fonctions d'onde diffusbes. On considere en particulier la d6pen-dance du temps'de vie T de la matrice crystalline, t i temperature tres baisse constante, et de la temperature T dans une matrice crystalline donnee. On reporte des donnees experimentaux de haut precision pour t ( T ) en Pu'aC1, RbCl ct KBr.

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Section: Experimental Data and Discussionmentioning

confidence: 99%

A contribution to the discussion on the relaxed excited F centre

Bosi¹,

Cova²,

Spinolo³

1975

Physica Status Solidi (b)

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“…From statistical considerations, linearity in the PMT response is expected to be within I% up to a counting rate of 250 KHz. 6 Linearity was experimentally observed up to about 300 KHz. Since most of the experiments with this instrument are at very low light levels, a few KHz at most, the dark count rate of the XP2230 of about 25 Hz at room temperature is a more significant contribution to the signal than pulse pileup or any other nonlinearities in the PMT response.…”

Section: Optics and Signal Detectionmentioning

confidence: 97%

Microprocessor-controlled photon-counting spectrofluorometer

Gratton

Limkeman

1983

Review of Scientific Instruments

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The construction details and performance characteristics for a fluorescence spectrophotometer are described. The system utilizes proton counting for signal detection and an Apple II microcomputer for data acquisition and analysis. Data-acquisition techniques are given along with typical excitation, emission, and polarization spectra. Particular reference is made to application of the instrument to fluorescence studies of protein/polymer films.

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“…In particular, different relative errors in measurements of the total (fluorescence and background, S + B) and the background respectively cause an amplified relative error (see e.g. Cova et al, 1973) in the result of the subtraction for cases when S/'B c~ is low, i.e. a < I.…”

Section: Introductionmentioning

confidence: 98%

“…Besides an increase in noise, these causes also produce a contribution to the average value, which will be called background (B). The signal-to-noise (S/N) ratio, ~m, and signal-to-background (S/B) ratio, a, are accordingly defined (see, e.g., Cova et al, 1973) as the ratios of the average value of the quantity of interest to the r.m.s, fluctuation a and to the background respectively.…”

Section: Introductionmentioning

confidence: 99%

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Digital microspectrofluorometry by multichannel scaling and single photon detection

Cova¹,

Prenna

Mazzini

1974

Histochem J

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Synopsis. A careful analysis of the requirements for microspectrofluorometric measurements has been made. General problems encountered in the measurement of light intensity and spectral distributions are considered, together with some special factors arising when measuring the fluorescence emitted by single cells. Criteria for evaluating microspectrofluorometric apparatus are given, and the relative merits of analog and digital methods are compared.A fully digital instrument using single-photon detection and a multichannel analyser is described. Automatic procedures for obtaining photodecomposition-fime curves and emission spectra are illustrated in detail. Other possible applications of the instrument are also outlined. The experimental results reported show that microspectrofluorometric measurements of high sensitivity are possible, as light intensities down to a few photons per second can be measured.

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The measurement of luminescence waveforms by single-photon techniques

Cited by 49 publications

References 33 publications

A contribution to the discussion on the relaxed excited F centre

A contribution to the discussion on the relaxed excited F centre

Microprocessor-controlled photon-counting spectrofluorometer

Digital microspectrofluorometry by multichannel scaling and single photon detection

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