The Measurement of Parathyroid Hormone

Wood, P J

doi:10.1177/000456329202900103

Cited by 28 publications

(8 citation statements)

References 64 publications

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“…Figure 1 shows that after 12 h there was a decrease of PTH concentrations by approximately 10% in serum samples, but concentrations in EDTA±plasma remained constant up to this time. This is in agreement with a previous study 2 in which investigators found that PTH concentrations in whole blood (without…”

supporting

confidence: 94%

Stability of parathyroid hormone in blood from renal patients on haemodialysis

Walker

Seth²

2000

Ann Clin Biochem

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supporting

confidence: 94%

Stability of parathyroid hormone in blood from renal patients on haemodialysis

Walker

Seth²

2000

Ann Clin Biochem

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“…Fifteen papers relating to PTH stability in whole blood were identified that had used a second generation assay ( Figure 2, Supplemental Data Table 3) [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Collation of study results was difficult due to variation in experimental design (see above), but almost all studies suggested that PTH was more stable in whole blood samples containing EDTA than in anticoagulant-free samples (Supplemental Data Table 3).…”

Section: Stability In Anticoagulated and Clotted Whole Blood At Room mentioning

confidence: 99%

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Hanon

Sturgeon

Lamb

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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Parathyroid hormone (PTH) is relatively unstable: optimisation of pre-analytical conditions, including specimen type, sampling time and storage conditions, is essential. We have undertaken a systematic review of these pre-analytical conditions. An electronic search of the PubMed, Embase, Cochrane, Centre for Research and Dissemination and Bandolier databases was undertaken. Of 5511 papers identified, 96 underwent full text review, of which 83 were finally included. At room temperature PTH was stable in ethylenediaminetetraacetic acid (EDTA) preserved whole blood for at least 24 h and in EDTA plasma for at least 48 h after venepuncture. Losses were observed in clotted blood samples after 3 h and in serum after 2 h. At 4°C PTH was more stable in EDTA plasma (at least 72 h) than serum (at least 24 h). Central venous PTH concentrations were higher than peripheral venous concentrations. In the northern hemisphere, PTH concentrations were higher in winter than summer. PTH has a circadian rhythm characterised by a nocturnal acrophase and mid-morning nadir. Data related to frozen storage of PTH (−20°C and −80°C) were limited and contradictory. We recommend that blood samples for PTH measurement should be taken into tubes containing EDTA, ideally between 10:00 and 16:00, and plasma separated within 24 h of venepuncture. Plasma samples should be stored at 4°C and analysed within 72 h of venepuncture. Particular regard must be paid to the venepuncture site when interpreting PTH concentration. Further research is required to clarify the suitability of freezing samples prior to PTH measurement.

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“…[40]. De acuerdo al sistema de detección utilizado estos ensayos pueden ser inmunoradiométricos, cuando el segundo anticuerpo está radiomarcado, e inmunoquimioluminiscentes cuando la marcación se hace con un compuesto quimioluminiscente [36,41].…”

Section: Técnicas Analíticasunclassified

“…Retraso en la congelación del suero por dos a cuatro horas (pérdida 4%-8%) [41] Retraso en la separación de las células (pérdida 6%)…”

Section: Analíticosunclassified

Metabolismo mineral y óseo: visión general y sus métodos de medición

Restrepo-Giraldo¹,

Arévalo-Novoa²,

Toro-Ramos

2015

Med. Lab.

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Las concentraciones plasmáticas de calcio, fósforo y magnesio dependen del balance neto del depósito mineral óseo y su resorción, la absorción intestinal y la excreción renal. Estos iones son importantes para muchas funciones biológicas y celulares como la señalización intracelular, la transmisión neural y la contracción muscular. Las principales hormonas que regulan la homeostasis de estos procesos son la hormona paratiroidea (PTH), la calcitonina, la 1,25-dihidroxi vitamina D y el factor de crecimiento fibroblástico-23 (FGF- 23). A través de sus acciones e interacciones sobre el hueso, el riñón y el tracto gastrointestinal las hormonas calciotrópicas (la hormona paratiroidea, la calcitonina y los metabolitos de la vitamina D, especialmente la 1,25-dihidroxi vitamina D) actúan para mantener la calcemia dentro de un rango normal, lo que permite el funcionamiento óptimo de muchos procesos fisiológicos dependientes de calcio. Los avances en las técnicas de análisis de los diferentes componentes del metabolismo mineral y óseo son útiles en la comprensión de su papel en la salud y la enfermedad. En este artículo se ofrece una revisión de los aspectos fisiológicos, clínicos y analíticos de estos protagonistas en el metabolismo óseo y mineral.

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The Measurement of Parathyroid Hormone

Cited by 28 publications

References 64 publications

Stability of parathyroid hormone in blood from renal patients on haemodialysis

Stability of parathyroid hormone in blood from renal patients on haemodialysis

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Metabolismo mineral y óseo: visión general y sus métodos de medición

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