The measurement of the absorption spectra of chlorine e 6 photosensitizer and hemoglobin in whole blood by multiple light scattering

Khairullina, A. Ya.; Parhotz, M. V.; Oleinik, T. V.; Bui, Lilia; Джагаров, Б. М.

doi:10.1134/1.1388323

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…This shift is commonly ascribed to the transfer of Ce6 ring from water to the less polar microenvironment ( e.g . for Ce6 complexes with serum proteins and PVP or its solutions in ethanol or DMSO ). It is known that Pluronic F127 forms small micelles ( R h ~11 nm ) in water solution.…”

Section: Resultsmentioning

confidence: 99%

Complexes of Chlorin e6 with Pluronics and Polyvinylpyrrolidone: Structure and Photodynamic Activity in Cell Culture

Zhiyentayev

Boltaev

Соловьева

et al. 2013

Photochem & Photobiology

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Polymeric carriers are extensively used in photodynamic therapy (PDT) for increase of efficacy of photosensitizers. Here, we report the influence of nine Pluronic copolymers on phototoxicity of chlorin e6 (Ce6), in particular 5- to 7-fold rise in the phototoxicity caused by hydrophilic Pluronics F127, F108, F68 and F87 and practically no influence on Ce6 of more hydrophobic polymers. The revealed value of 0.2 mg mL(-1) of Pluronic F127 concentration sufficient for half-of-maximal increase of Ce6 photodynamic activity proved to be close to 0.16 mg mL(-1) inherent in well-documented carrier poly(N-vinylpyrrolidone) (PVP). The dissociation constants of Ce6 complexes with Pluronic F127 and PVP that were estimated from UV spectra were 0.252 and 0.036 mg mL(-1) , respectively, indicating higher stability of Ce6 complex with PVP. According to the results of (1) H-NMR studies of Ce6 complexes, the porphyrin interacts not only with hydrophobic regions but also with hydrophilic sides of both polymers.

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