The measurement of vitamin D metabolites part II—the measurement of the various vitamin D metabolites

Makris, Konstantinos; Sempos, Christopher T.; Cavalier, Étienne

doi:10.1007/s42000-020-00188-9

Cited by 14 publications

(10 citation statements)

References 90 publications

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“…Please note that this list is not exhaustive and features a selection by the present authors; also note that additional studies are mentioned throughout this review. We refer the reader to the first installment of this review (Volmer et al, 2015) and other publications (Altieri et al, 2020; Makris et al, 2020; van den Ouweland et al, 2013; van den Ouweland, 2016), where many more excellent LC‐MS/MS assays for vitamin D metabolites are summarized. Gas chromatography (GC) has also been employed for vitamin D measurement (Lehner et al, 2021; Yang et al, 2019), but this separation technique is out of the scope of this review.…”

Section: Recent Instrumental Progressmentioning

confidence: 99%

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Αλεξανδρίδου

Schorr

Stokes

et al. 2021

Mass Spectrometry Reviews

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Liquid chromatography/tandem mass spectrometry is firmly established today as the gold standard technique for analysis of vitamin D, both for vitamin D status assessments as well as for measuring complex and intricate vitamin D metabolic fingerprints. While the actual mass spectrometry technology has seen only incremental performance increases in recent years, there have been major, very impactful changes in the front‐ and back‐end of MS‐based vitamin D assays; for example, the extension to new types of biological sample matrices analyzed for an increasing number of different vitamin D metabolites, novel sample preparation techniques, new powerful chemical derivatization reagents, as well the continued integration of high resolution mass spectrometers into clinical laboratories, replacing established triple‐quadrupole instruments. At the same time, the sustainability of mass spectrometry operation in the vitamin D field is now firmly established through proven analytical harmonization and standardization programs. The present review summarizes the most important of these recent developments.

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Section: Recent Instrumental Progressmentioning

confidence: 99%

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Αλεξανδρίδου

Schorr

Stokes

et al. 2021

Mass Spectrometry Reviews

View full text Add to dashboard Cite

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“…LC–MS/MS is often described as the ‘gold standard’ method for vitamin D analysis as it provides increased sensitivity and specificity over other assays [ 4 ] as well as the ability to analyse multiple metabolites simultaneously [ 8 ]; however, it often requires trained laboratory personnel. Immunoassays dominate 25(OH)D determination in clinical laboratories [ 6 ] because they are fully automated and offer much faster measurements of 25(OH)D levels than LC–MS/MS methods.…”

Section: Introductionmentioning

confidence: 99%

Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC–MS/MS analysis

Αλεξανδρίδου

Volmer

2022

Anal Bioanal Chem

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The determination of vitamin D metabolites as status marker or for diagnostic purposes is almost entirely conducted from blood serum or plasma. Other biological matrices, however, have also interested researchers, for two main reasons: (1) alternative matrices may allow non-invasive sampling, permit easier sample transfer and require less demanding storage conditions; and (2) the levels of vitamin D metabolites in other body compartments may further aid the understanding of vitamin D metabolism and function. Thus, the development of reliable and efficient sample preparation protocols for sample matrices other than serum/plasma, which will remove potential interferences and selectively extract the targeted metabolites, is of great importance. This review summarizes sample preparation methods for measurement of vitamin D metabolites using liquid chromatography-(tandem)mass spectrometry in more than ten different human tissues, including hair, saliva, adipose tissue, brain and others. Graphical abstract

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“…Nevertheless, the reliability of this indicator for vitamin D status has been questioned as it may not reflect the complex vitamin D metabolism. Several external factors influence serum and plasma 25(OH)D 3 levels, such as the sampling period of the year, geographical location where individuals live, ethnicity and exposure to sunlight [3]. Moreover, endogenous factors such as obesity, starvation, diabetes and glucocorticoids have a major influence on CYP2R1 activity [4].…”

Section: Introductionmentioning

confidence: 99%

“…Participants with 25(OH) 2 D 3 serum levels below 12 ng mL −1 2. Participants with 25(OH) 2 D 3 serum levels between 12 and 20 ng mL −1 3. Participants with 25(OH) 2 D 3 serum levels above 20 ng mL −1 .…”

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confidence: 99%

Measuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals

et al. 2022

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The main branch of vitamin D3 metabolism involves several hydroxylation reactions to obtain mono-, di- and trihydroxylated metabolites, including the circulating and active forms—25(OH)D3 and 1,25(OH)2D3, respectively. However, most clinical trials strictly target the determination of 25(OH)D3 to offer a view of the metabolic status of vitamin D3. Due to the growing interest in expanding this restricted view, we have developed a method for measuring vitamin D3 metabolism by determination of vitamin D3, 25(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,24,25(OH)3D3 in human plasma. The method was based on SPE–LC–MS/MS with a large volume injection of human plasma (240 µL). Detection of di- and trihydroxymetabolites, found at the picogram per milliliter level, was attained by the combined action of high preconcentration and clean-up effects. The method allows obtaining information about ratios such as the known vitamin D metabolite ratio (24,25(OH)2D3/25(OH)D3), which can provide complementary views of vitamin D3 metabolic status. The method was applied to a cohort of obese patients and a reference cohort of healthy volunteers to find metabolic correlations between target analytes as well as differences as a function of vitamin D levels within and between cohorts.

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The measurement of vitamin D metabolites part II—the measurement of the various vitamin D metabolites

Cited by 14 publications

References 90 publications

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC–MS/MS analysis

Measuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals

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