Spinal fusion with pedicle-screw-rod is being used widely for treating spinal deformities diseases. Several biomechanical studies on screw rod based implant failure through screw pullout, bending, screw breakage have been performed. But few studies are available regarding the effect of strain for breakage of rod. So, the purpose of the present study is to observe strain at the rod connected with the pedicle screw for different loading condition. The strain in stainless steel (SS) connecting rods for pedicle screw fixation were measured using strain gauge. In order to investigate the bio-mechanical response of lumbar spine with reference to strain in the rod, a simple experimental setup was developed using a specimen of L1-S spine segment. SS rods were used for pedicle screw implant on prototyped lumbar Spine. Prior to testing with pedicle screw, the lumbar spine specimen was also compared with FE results. The strain measured using strain gauges at L3-L4 level on SS rod were within a range of 85 to 310 microstrain under 6, 8, 10 Nm flexion and extension, and for L4-L5 level, these values were within a range of 95 to 440 microstrain. It was found that FE result was higher than the strain gauge result and the error varied between 10.5% to 33% with average error of 22.8%. However similar stain behavior was observed by the FE analysis. The proposed method, as well as the qualitative data, might be helpful for the researchers to understand biomechanical behavior of pedicle-screw implanted spine.