The mechanical fingerprint of murine excisional wounds

Pensalfini, Marco; Haertel, Eric; Hopf, Raoul; Wietecha, Mateusz S.; Werner, Sabine; Mazza, Edoardo

doi:10.1016/j.actbio.2017.10.021

Cited by 28 publications

(38 citation statements)

References 39 publications

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“…To further analyze the scar tissue, we excised skin specimens at 21d post-injury and performed uniaxial tensile tests in a custombuilt setup allowing for local deformation analysis 17 . We quantified the level of deformation in the scar tissue corresponding to a 10% deformation in the nearby-unwounded skin, and confirmed the reduced deformability of Act scars (Fig.…”

Section: Activin Changes the Biomechanical Properties Of Skin Woundsmentioning

confidence: 99%

“…Most published studies on wound biomechanics determined rupture properties of healing tissue, thereby applying largely overphysiological deformations 15,16 . We recently established an ex vivo protocol to characterize wound stiffness at physiological stretch magnitude 17 . So far, the only in vivo investigation providing a local characterization of the mechanical behavior of skin wounds was based on indentation, which pushes the skin downwards.…”

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Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds

et al. 2020

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Matrix deposition is essential for wound repair, but when excessive, leads to hypertrophic scars and fibrosis. The factors that control matrix deposition in skin wounds have only partially been identified and the consequences of matrix alterations for the mechanical properties of wounds are largely unknown. Here, we report how a single diffusible factor, activin A, affects the healing process across scales. Bioinformatics analysis of wound fibroblast transcriptome data combined with biochemical and histopathological analyses of wounds and functional in vitro studies identify that activin promotes pro-fibrotic gene expression signatures and processes, including glycoprotein and proteoglycan biosynthesis, collagen deposition, and altered collagen cross-linking. As a consequence, activin strongly reduces the wound and scar deformability, as identified by a non-invasive in vivo method for biomechanical analysis. These results provide mechanistic insight into the roles of activin in wound repair and fibrosis and identify the functional consequences of alterations in the wound matrisome at the biomechanical level.

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Section: Activin Changes the Biomechanical Properties Of Skin Woundsmentioning

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Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds

et al. 2020

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“…When using animal (usually rodent) models for wound healing in humans, it is important to realize that while the closure of human wounds is primarily accomplished through proliferation and migration of cells at the wound edge, contraction is the driving force behind wound closure in rodents (Pensalfini et al 2018). Nevertheless, the major cellular and molecular processes that occur during healing are conserved between both species and allow for rodent wounds to serve as a model for human wound repair (Galiano et al 2004;Gurtner et al 2008).…”

Section: Definition and Phasesmentioning

confidence: 99%

Effect of n-3 long-chain polyunsaturated fatty acids on wound healing using animal models – a review

Komprda

2018

Acta Vet. Brno

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The present review summarizes results of experiments, mostly performed on rodents, regarding the effects of fish oil (FO) and its biologically active constituents, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the healing of cutaneous wounds, but also of selected other types of injury. Structure, metabolism and functions of EPA/DHA in an organism are briefly mentioned, with an emphasis on the ability of these long-chain polyunsaturated fatty acids to modulate inflammation. Wound healing as a complex programmed sequence of cellular and molecular processes including inflammation, cell migration, angiogenesis, synthesis of provisional matrix, collagen deposition and reepithelialisation is briefly described. Markers for evaluation of the healing process include planimetry indices, tensile strength, quantification of collagen synthesis including hydroxyproline determination, histopathology/immunohistochemistry and genomic/proteomic markers. As far as effects on wound healing are concerned, the main emphasis is put on the outcomes of experiments using a dietary FO/DHA/EPA administration, but the results of experiments with a parenteral application are also mentioned, together with selected relevant in vitro studies. An important conclusion from the above-mentioned studies is an inconsistency of FO/DHA/EPA effects on wound healing: decreased/increased collagen deposition; lower/higher counts of the inflammatory cells in the healing tissue; increased/decreased concentration of both pro- and anti-inflammatory cytokines; DHA accelerated/delayed wound healing process. Some experiments indicate superiority of DHA over EPA regarding wound healing.

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“…In large wounds, both concentric constriction of wound margins and radial contraction of the maturing wound granulation tissue play a role in wound closure, particularly in looseskinned mammals. Furthermore, a recent examination of the tensional environment of linear wounds in mice showed that the central portion is shielded from strain by a peripheral cushion (Pensalfini et al, 2018). By analogy, the larger size of the excisional wound in the thin-skinned mouse may allow development of a mechanical shield around the central region.…”

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“…However, the differences in dermal architecture between the mouse and rat predict markedly different biomechanical environments. Noninvasive and destructive measurement strategies (Guan et al, 2004;Pensalfini et al, 2018;Staloff and Rafailovitch, 2008;Wong et al, 2012) would allow the correlation of multidimensional wound biomechanics with other aspects of skin regeneration. These physical data, together with a better characterization of the extracellular matrix, could lead to a more comprehensive understanding of how the extracellular and intracellular environments interact to permit initiation and completion of a regenerative sequence.…”

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Hair Regeneration under Stress

Davidson

2018

Journal of Investigative Dermatology

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The phenomenon of wound-induced hair neogenesis in adult mice and rabbits offers a tantalizing window into the mechanisms of regeneration. By comparing wounds in mice and several rat strains, Guerrero-Juarez et al. attempted to identify factors that may contribute to the failure of wound-induced hair neogenesis to occur in the rat. In addition to biochemical, cellular, and molecular variation, worthwhile comparisons could include the magnitude, distribution, and source of tensional forces within the wound environment.

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The mechanical fingerprint of murine excisional wounds

Cited by 28 publications

References 39 publications

Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds

Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds

Effect of n-3 long-chain polyunsaturated fatty acids on wound healing using animal models – a review

Hair Regeneration under Stress

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