The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

Abstract

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

Incorporation of catechin extracts from gambier products and pasak bumi in the production of functional instant green robusta coffee

“…It has been reported that both robusta and arabica generally contain functional compounds in chlorogenic acid. This compound was also discovered by [34] to be present in coffee as an antioxidant, with robusta reported by [38] to contain higher content at 43. 63% than arabica, which has 36.18%.…”

Current Research of Phytochemical, Medicinal and Non-Medicinal Uses of Uncaria gambir Roxb.: A Review

“…Rahmawati and Fernando [ 67 ] investigated the phenolic content and antioxidant activity of the ethyl acetate extract of dried gambir leaves using the maceration method. Phenolic content using the Folin–Ciocalteu method, obtained phenolic content of 172.62 mg GAE/mL at a temperature of 80 °C, with an antioxidant activity using the DPPH method of 28.34 ppm.…”

Effects of Dietary Catechin Uncaria gambir Extract on Growth Performance, Carcass Characteristics, Plasma Lipids, Antioxidant Activity and Nutrient Digestibility in Broiler Chickens

“…The gambir is an extraction product of the gambir plant (Uncaria gambir Roxb) from the Rubinace family, a potential catechin producer from Indonesia. Gambir contains many monomeric flavonoids, almost totally catechins, and a trace of epicatechin (Widiyarti et al 2020). Gambir catechin purification has been successfully carried out (Yeni et al 2014;Ferdinal 2014) through 2-stage extraction.…”