The mechanism of action of reactivating factor from Luteococcus japonicus subsp. casei

Vorob'eva, L. I.; Khodzhaev, E. Yu.; Mulyukin, A. L.; Toropygin, I. Yu.

doi:10.1134/s0003683809050056

Cited by 9 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficacy of cryoprotectors was diminished in the row of CL, K, RF, and RF+K after 3 months, which means that the RF overcame the other cryoprotectors in their effect on cell survival over a long time period. As was shown previously, the RF effect can be associ ated with the reparation of damaged DNA and the recovery of the cytoplasmic membrane [9]. Protection and reactivation of lactic acid bacteria exposed to bile salts.…”

Section: Resultsmentioning

confidence: 87%

“…The protective effect is possibly associated with the signaling mechanism [9]; however, the pep tide antioxidative activity cannot be excluded. The antioxidative activity is thought to be associated with the multifunctionality of the lactoferrin low molecu lar peptide, which contains redox active amino acid residues.…”

Section: Resultsmentioning

confidence: 99%

“…For the RF isolation, the L. casei culture in the end of the exponential growth (48 h) was centrifuged at 10000 g for 20 min, cells were washed and resuspended in the 0.05 M Na phosphate buffer (pH 7.4). The RF was purified from the bacterial CL as described previ ously [9].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Biological effect of extracellular peptide factor from Luteococcus japonicus subsp. casei on probiotic bacteria

Vorobjeva

Khodjaev

Kharchenko

et al. 2014

Appl Biochem Microbiol

View full text Add to dashboard Cite

The biological effect of the extracellular peptide reactivating factor (RF) from Luteococcus casei on cells of probiotic cultures was studied. The RF showed the protective and reactivating effects on the Bifi dobacterium bifidum cells under the action of bile salts and an acidic stress. Also, it acted as a cryoprotector during lyophilisation and long term culture storage. The RF and the L. casei culture liquid (CL) were shown to have bifidogenic properties. The degree of protection and reactivation of lactic acid bacteria under the action of bile salts depended on the particular strain properties. The maximum degree of protection (more than thirteen fold) and reactivation (close to three fold) was found in Lactobacillus casei, while the minimum values were characteristic of Lactobacillus reuterii. The resistance of lactobacilli to bile was increased in the row of L. acidophilus, L. casei, L. plantarum, L. rhamnosus, and L. reuterii correlating with the RF protection degree.

show abstract

Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%