The mechanism of action of coculture on embryo development in the mouse model: direct embryo-to-cell contact and the removal of deleterious components

Joo, Bo Sun; Kim, Mi Kyung; Na, Yong Jin; Moon, Heui Soo; Lee, Kyu Sup; Kim, Han Do

doi:10.1016/s0015-0282(00)01671-x

Cited by 43 publications

(38 citation statements)

References 28 publications

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“…Indeed, in term of cleavage and speed of embryo development, co-culture system had not only superiority over cell free culture system (SOF) but also, except for OECs, embryo development was deteriorated by culturing in MSCs, EFCs, and late co-cultured groups (SOF-MSCs and SOF-EFCs) ( Table 1). This finding was contrasted to the majority of reports indicating the faster cleavage and higher blastocyst rate in co-culture systems (Bongso et al, 1989;Wiemer et al, 1989;Ellington et al, 1990;Smith et al, 1992;Wetzels et al, 1998;Joo et al, 2001). Though, there are some reports indicating no significant improvement in early embryogenesis (Tucker et al, 1995;Hu et al, 1998) and even reports indicating the adverse effects of co-culture on pre-implantation embryo development (Bernardi et al, 1996).…”

Section: Discussionmentioning

confidence: 70%

“…such as: higher and faster cleavage (Bongso et al, 1989), improved morphological appearance/ grade (Wiemer et al, 1989), increase of the average number of blastomeres (Smith et al, 1992), improved post-thaw blastomere survival of cryopreserved co-cultured embryos (Tucker et al, 1995), reduced apoptosis (Xu et al, 2000), higher blastocyst rate (Joo et al, 2001), facilitated hatching (Ellington et al, 1990), lower fragmentation rates, improved pregnancy rates (Wiemer et al, 1989), higher implantation ratio (Wetzels et al, 1998), and live births (Marcus and Brinsden, 1996). These effects are most pronounced with increasing the duration of co-culture (Wiemer et al, 1989), especially during the early cleavage stages which may be mediated by the expression of growth factors (Yeung et al, 1992).…”

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confidence: 99%

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Effect of various co-culture systems on embryo development in ovine

Heidari¹,

Shirazi²,

Naderi³

et al. 2013

CZECH J ANIM SCI

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Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey's test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups.

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Section: Discussionmentioning

confidence: 70%

mentioning

confidence: 99%

Effect of various co-culture systems on embryo development in ovine

Heidari¹,

Shirazi²,

Naderi³

et al. 2013

CZECH J ANIM SCI

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“…The co-culture system overcomes the 2-cell block in vitro and improves the embryo development. The beneficial effect may be a result of direct cell-to-cell contact between the embryo and helper cells and the removal of deleterious components from medium (Joo et al, 2001). …”

Section: Discussionmentioning

confidence: 99%

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Zhou

Shi

et al. 2008

Animal

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The aims of the study were to measure the mRNA expression of brain-derived neurotrophic factor (BDNF) in bovine oocytes and early embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA) and nuclear transfer (NT), and to investigate the effects of BDNF on the development of IVF and parthenogenetic embryos. Bovine oocytes matured in vitro for 22 h were in vitro fertilized or parthenogenetic activated. By reverse transcription-PCR and quantitative real-time PCR, we found that germinal vesicle (GV) oocytes, metaphase II (MII) oocytes, 4-cell and 8-cell embryos, morulae, and blastocysts were all shown to express mRNA for BDNF. The mRNA levels for BDNF gene were different in bovine oocytes and IVF embryos at different stages (P , 0.01), with the highest expression in MII oocytes and the lowest expression in 8-cell embryos. The mRNA for BDNF was highly expressed in the PA and IVF blastocysts compared to the NT blastocysts (P , 0.01). Supplementation of culture media with BDNF at the concentration of 40 mg/l caused a significant increase in the rates of in vitro-fertilized blastocyst formation (P , 0.05) and parthenogenetic blastocyst formation (P , 0.05). However, the rate of oocyte cleavage in BDNF groups was not significantly different from that in the BDNF-free control (P . 0.05) after IVF or PA. We have also investigated the effects of BDNF on the growth of granulosa cells, which were used for co-culture of bovine early embryos. The results revealed that supplementation of culture media with 20 mg/l BDNF promoted the growth of granulosa cells (P , 0.01). Taken together, these results provided evidence for the role of neurotrophins in promoting early embryonic development as well as in the growth of granulosa cells by the co-culture system, indicating that BDNF can directly or indirectly promote bovine early embryo development.

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“…In the first of them, it is possible that the number of embryos maintained in reduced culture was too high (16 embryos) for observing a significant effect in apoptosis, although a reduction in the number of in vitro cultured bovine embryos, from 50 to 16 embryos, significantly decreased the blastocyst rate (Hoelker et al, 2009). In the second one, singly cultured embryos were cultured in the presence of a cumulus cell monolayer (Goovaerts et al, 2011) and it was observed that oviductal cell coculture diminished the superoxide anion level in embryo CM (Joo et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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The mechanism of action of coculture on embryo development in the mouse model: direct embryo-to-cell contact and the removal of deleterious components

Cited by 43 publications

References 28 publications

Effect of various co-culture systems on embryo development in ovine

Effect of various co-culture systems on embryo development in ovine

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

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