The mechanism of ammonia inhibition in the thermophilic digestion of livestock wastes

Wiegant, W.M.; Zeeman, G.

doi:10.1016/0141-4607(86)90056-9

Cited by 114 publications

(47 citation statements)

References 19 publications

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“…The information about the sensitivity to ammonia concentrations of acetoclastic and hydrogenotrophic methanogens in literatures was conflicting [27]. In the present study, Methanothermobacter thermautotrophicus, which is a hydrogenotrophic methanogens, showed relatively higher tolerance to the inhibition, and this result was in accordance with the study of Wiegant and Zeeman [28], which indicated that ammonia has stronger inhibition to the formation of methane from H 2 and CO 2 . Bands A6 and A7 disappeared in Run 1 due to the inhibi- tion, and it showed the same behavior in Run 2.…”

Section: Microbial Community Structure Change During Digestionsupporting

confidence: 82%

Methane Recovery and Microbial Community Analysis of a High Solid Thermophilic Co-digestion of Sewage Sludge and Waste Fried Tofu

Togari

Tsuchiya-Nakakihara

et al. 2016

J. of Wat. & Envir. Tech.

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A continuous high solid co-digestion experiment of dewatered sludge from an oxidation ditch process and waste fried tofu was conducted at 55°C in the present study. Sludge retention time was 10 days, and the mixing ratio of sludge, dry and raw fried tofu was 1:0.39:0.06 (dry weight basis). Total solids (TS) of substrate was increased gradually (30, 50, 70, 100 g/L) in the sludge digestion, while TS was increased and then decreased in the co-digestion (43.5, 72.5, 101.5, 72.5 g/L). In the sludge digestion, performance was stable when the TS was below 70 g/L (organic loading rate (OLR) was 5.9 kg-volatile solids (VS)/(m 3 ·d)), and methane yields of sludge were 0.05-0.08 L/g-TS. In the co-digestion, biogas production significantly increased by the fried tofu addition. When total TS was increased to 101.5 g/L (OLR 8.8 kg-VS/(m 3 ·d)), accumulation of ammonia and volatile fatty acid was observed, inhibition was indicated. Methane fermentation failed to recover when the TS was decreased to 72.5 g/L (OLR 6.3 kg-VS/(m 3 ·d)). Results of the denaturing gradient gel electrophoresis analysis of the microbial community revealed a significant difference in the bacterial community with the fried tofu addition, while no major difference in the archaeal community was recognized.

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Section: Microbial Community Structure Change During Digestionsupporting

confidence: 82%

Methane Recovery and Microbial Community Analysis of a High Solid Thermophilic Co-digestion of Sewage Sludge and Waste Fried Tofu

Togari

Tsuchiya-Nakakihara

et al. 2016

J. of Wat. & Envir. Tech.

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“…Supporting materials prevent ammonia inhibition and acetate accumulation by proliferating aceticlastic methanogens in the inner region, although hydrogenotrophic methanogens can grow even in suspended fractions at a free ammonia level of 0.29 gN/l (Sasaki et al 2011a). However, Wiegant and Zeeman (1986) observed that hydrogenotrophic methanogens were more sensitive to ammonia than aceticlastic methanogens. Research evidence also shows that syntrophic decomposition by acetate-oxidizing bacteria and hydrogenotrophic methanogens dominate in mesophilic conditions under high ammonia levels (Schnürer and Nordberg 2008).…”

Section: Ammonia Inhibitionmentioning

confidence: 90%

Factors influencing the degradation of garbage in methanogenic bioreactors and impacts on biogas formation

Murakami

Sasaki

2012

Appl Microbiol Biotechnol

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Anaerobic digestion of garbage is attracting much attention because of its application in waste volume reduction and the recovery of biogas for use as an energy source. In this review, various factors influencing the degradation of garbage and the production of biogas are discussed. The surface hydrophobicity and porosity of supporting materials are important factors in retaining microorganisms such as aceticlastic methanogens and in attaining a higher degradation of garbage and a higher production of biogas. Ammonia concentration, changes in environmental parameters such as temperature and pH, and adaptation of microbial community to ammonia have been related to ammonia inhibition. The effects of drawing electrons from the methanogenic community and donating electrons into the methanogenic community on methane production have been shown in microbial fuel cells and bioelectrochemical reactors. The influences of trace elements, phase separation, and co-digestion are also summarized in this review.

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“…It is therefore common to ®nd a wide range of values reported at which the anaerobic digestion process is deemed to have been inhibited by ammonia/amonium ion [25,26]. It is, however, well accepted that toxicity effects may be detrimental to the anaerobic process performance in both homoge- [27,28] has been found to be very dif®cult at ammonia nitrogen concentration of 3 g/l; in these cases the authors attributed these dif®culties to ammonia toxicity rather than high volatile solids loadings. The effect is likely to be more pronounced on the methanogenic population in the digester as these are reported to have the greatest sensitivity [29], Methanobacterium formicum, for example, was shown to be at least partially inhibited at an ammoniacal nitrogen concentration of 3.3 g/l.…”

Section: Kinetics Of Methane Productionmentioning

confidence: 99%

Effect of temperature and pH on the kinetics of methane production, organic nitrogen and phosphorus removal in the batch anaerobic digestion process of cattle manure

Sánchez¹,

Borja

Weiland³

et al. 2000

Bioprocess Engineering

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A study of the effect of temperature and pH on the kinetics of methane production and organic nitrogen and phosphorus degradation in the anaerobic digestion process of cattle manure was carried out. Two laboratoryscale batch completely mixed reactors, operating at 35°C (mesophilic temperature), and other two, operating at 60°C (thermophilic temperature) were used. For each temperature selected, the in¯uent pH values were 7.6 (initial pH of the waste used) and 7.0. The apparent kinetic constants of the biomethanization process increased 2.3 times when the initial pH of the in¯uent was increased from 7.0 to 7.6 at mesophilic temperature. The values found at thermophilic temperature were similar. The kinetic constants of methane production decreased 2.6 and 7.2 times when the operating temperature increased from 35°C to 60°C for the experiments carried out at initial pH of 7.0 and 7.6, respectively. The methane yield coef®cient (l CH 4 STP/g VS removed) also decreased when the temperature increased from 35°C to 60°C for the two initial pH values studied. This behaviour agreed with the major inhibition level observed at thermophilic temperature as a result of the higher organic nitrogen removal and ammonia nitrogen production observed at 60°C. Speci®-cally, the speci®c rate constants for organic nitrogen removal and ammonia nitrogen production increased 3.6 and 12 times when the temperature was increased from 35°C to 60°C for the experiments carried out at initial pH values of 7.0 and 7.6, respectively. In the same way, the values of the kinetic constant for phosphorus removal were 44% and 80% higher than those obtained at 35°C for the two initial pH values above-mentioned, respectively. Finally, the experimental values of organic nitrogen and phosphorus concentrations were reproduced with deviations equal to or less than 10% and 15% in every case, respectively.

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The mechanism of ammonia inhibition in the thermophilic digestion of livestock wastes

Cited by 114 publications

References 19 publications

Methane Recovery and Microbial Community Analysis of a High Solid Thermophilic Co-digestion of Sewage Sludge and Waste Fried Tofu

Methane Recovery and Microbial Community Analysis of a High Solid Thermophilic Co-digestion of Sewage Sludge and Waste Fried Tofu

Factors influencing the degradation of garbage in methanogenic bioreactors and impacts on biogas formation

Effect of temperature and pH on the kinetics of methane production, organic nitrogen and phosphorus removal in the batch anaerobic digestion process of cattle manure

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