The Mechanism of <i>Cornus officinalis</i> Total Glycosides and Cornus Polysaccharide on Myocardial Protection in Rats with Acute Myocardial Infarction

Abstract

Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were randomly divided into 5 groups: sham operation group, model group, preventive treatment group, COTG treatment group, CP treatment group, and there were 12 cases in each one. By legating the left anterior descending branch of coronary artery method, acute myocardial infarction model was established. The rat of sham operation group and model group was intragastric administered with physiological saline; other groups were given with corresponding drugs. The cardiac function, the myocardial infarct area, the expression of mitochondrial biogenesis genes such as PGC-1α, PGC-1β, NRF-1mRNA and GSK-3β mRNA, GSK-3β Protein Expression were analyzed. Results: The results revealed that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP, and −dp/dt decreased; LVSP increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05); LVEDV increased in preventive treatment group (p < 0.05), PGC 1 alpha, and PGC 1 beta; the NRF-1 mRNA expression increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with CP and COTG treatment group, PGClpha, beta PGC 1, the NRF-1 mRNA expression increased in preventive treatment group (p < 0.05). Compared with the sham operation group, GSK-3 beta mRNA and protein expression increased in model group, preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with model group, GSK-3 beta mRNA expression reduced in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Conclusions: Cornus officinalis total glycosides and Cornus polysaccharides can effectively * Corresponding author. K. Chen et al. 46 protect myocardial mitochondria of acute myocardial infarction rats by activating GSK-3β signaling pathways, and reduce the myocardial infarct size, which has great significance for improving cardiac function.

Cornus officinalis Sieb. et Zucc. Alleviates Mitochondrial Abnormalities and Heart Failure through AMPK

“…For thousands of years, Cor has been one of the most widely used medicinal plants in China and other East Asian countries to treat diseases, such as liver, kidney, and cardiovascular diseases and frequent urination (Hou et al, 2018). According to Chen et al (2015), the total glycosides and polysaccharides of Cor can improve cardiac function, reduce the area of acute myocardial infarction in rats, and promote the formation of myocardial mitochondria. In addition, Cor fruit core extract can reverse cardiac hypertrophy and improve cardiac functions (Fang et al, 2012).…”

Effects of Cornus Officinal Is Total Glycoside on Cardiomyocyte Apoptosis and Calcium Overload after Hypoxia/Reoxygenation Injury in Rats

“…Our previous studies revealed that Cornus officinalis Total Glycosides, a promising cardioprotective Chinese herbs extract, inhibit cardiomyocyte apoptosis of suckling mouse in vitro [12] and improve myocardial ischemia injury by promoting mitochondrial biogenesis [8]. Mitochondria are important organelles for the energy supply of cardiac myocytes.…”

“…et Zucc., which have anti-inflammatory effects [7]. Our previous studies have been proved that Cornus officinalis Total Glycosides can improve myocardial ischemia injury by promoting mitochondrial biogenesis [8]. Nevertheless, it is unclear about the effect of Cornus officinalis…”

A comprehensive review of Cornus officinalis: health benefits, phytochemistry, and pharmacological effects for functional drug and food development

“…In a study utilizing an acute myocardial infarction (AMI) rat model to investigate the impact of C. officinalis polysaccharides on AMI rats, the results, compared with the model group, revealed an increase in left ventricular systolic pressure (LVSP) and a significant reduction in the myocardial infarction area. This suggests that C. officinalis polysaccharides play a crucial role in enhancing cardiac function and reducing the size of myocardial infarcts, possibly through the activation of the GSK-3β signaling pathway, imparting a cardioprotective effect ( 101 ). Furthermore, research has explored the antibacterial and antioxidant properties of polysaccharides derived from C. officinalis leaves.…”