The mechanism of electronic excitation in the bacterial bioluminescent reaction

Nemtseva, Elena V.; Kudryasheva, Nadezhda S.

doi:10.1070/rc2007v076n01abeh003648

Cited by 51 publications

(51 citation statements)

References 38 publications

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“…One of them is the bioluminescence reaction which includes a peroxide compound (peroxyhemiacetal) as an intermediate [41]. The excess amount of reaction components is known to inhibit all the reactions and quench electron-excited states of fluorofores [42], thus inhibiting bioluminescence and other metabolic processes.…”

Section: Peroxides In Solution Of 241 Am(no 3 ) 3 and In Htomentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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Section: Peroxides In Solution Of 241 Am(no 3 ) 3 and In Htomentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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“…NMR studies have suggested that FMNH 2 binds to the enzyme in its anionic state (FMNH -) (Vervoort et al, 1986). With the flavin bound to the enzyme, molecular oxygen then binds to the C4a atom to form an intermediate 4a-hydroperoxy-5-hydroflavin (Nemtseva & Kudryasheva, 2007). It is important to note that this important C4a atom was determined to be in close proximity to a reactive thiol from the side chain of Cys 106 on the  subunit (Campbell, Z.T.…”

Section: Bacterial Luciferase Mechanism Of Actionmentioning

confidence: 99%

“…This complex then undergoes a transformation (through an unknown intermediate or series of intermediates) to an excited state generally accepted to be a luciferase-bound 4a-hydroxy-5-hydroflavin mononucleotide, which then decays to give oxidized FMN, a corresponding aliphatic acid, and light (Fig. 4) (Nemtseva & Kudryasheva, 2007). There have classically been many theories proposed to explain the exact process required for light emission that continue to expand today as technology for detecting the intermediate complexes has improved (Hastings, JW & Nealson, 1977;Nemtseva & Kudryasheva, 2007).…”

Section: Bacterial Luciferase Mechanism Of Actionmentioning

confidence: 99%

“…4) (Nemtseva & Kudryasheva, 2007). There have classically been many theories proposed to explain the exact process required for light emission that continue to expand today as technology for detecting the intermediate complexes has improved (Hastings, JW & Nealson, 1977;Nemtseva & Kudryasheva, 2007). A) The luciferase is formed from a heterodimer of the luxA and luxB gene products.…”

Section: Bacterial Luciferase Mechanism Of Actionmentioning

confidence: 99%

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Mammalian-Based Bioreporter Targets: Protein Expression for Bioluminescent and Fluorescent Detection in the Mammalian Cellular Background

Ripp¹,

Sayler²,

Close³

2011

Biosensors for Health, Environment and Biosecurity

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“…These are the reasons why luminous bacteria have been used as a toxicity bioassay for several decades (Grabert and Kossler, 1997;Roda et al, 2004;Girotti et al, 2008;Thakur and Ragavan, 2013;Esimbekova et al, 2014;Kudryasheva and Tarasova, 2015). It also explains the increasing interest and intensive research into the functions of luminous bacteria, their sensitivity to exogenous compounds (Girotti et al 2008;Deryabin and Aleshina, 2008;Hastings, 2012), and the mechanisms of light emission (Nemtseva and Kudryasheva, 2007;Hou et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

On the mechanism of biological activation by tritium

Rozhko

Badun

Razzhivina

et al. 2016

Journal of Environmental Radioactivity

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The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescent kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media. ON THE MECHANISM OF BIOLOGICAL ACTIVATION BY TRITIUM AbstractThe mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface 0.11, 0.28, 0.91, and 2.36 MBq/cm 2 ). When using the tritiumlabeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescent kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media.

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The mechanism of electronic excitation in the bacterial bioluminescent reaction

Cited by 51 publications

References 38 publications

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Mammalian-Based Bioreporter Targets: Protein Expression for Bioluminescent and Fluorescent Detection in the Mammalian Cellular Background

On the mechanism of biological activation by tritium

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