The mechanism of intestinal microbiota regulating immunity and inflammation in ischemic stroke and the role of natural botanical active ingredients in regulating intestinal microbiota: A review

Zeng, Jianwei; Yang, Kailin; Nie, Huifang; Le, Yuan; Wang, Shanshan; Zeng, Liuting; Ge, Anqi; Ge, Jinwen

doi:10.1016/j.biopha.2022.114026

Cited by 13 publications

(5 citation statements)

References 202 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the highly technical nature of these systems means the use of these systems is restricted to specialist labs. Since we deposited this manuscript as a pre-print in bioRxiv circa 2019, two independent groups have published transwell-based systems to coculture intestinal microbes with HIO monolayers [ 20 , 35 ]. Both these systems have unique strengths and limitations; for example, Sasaki et al [ 20 ] describe a system that is relatively simple but requires inserting plugs that are not widely available, and the basal compartment is not physoxic.…”

Section: Discussionmentioning

confidence: 99%

A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction

Fofanova,

Karandikar,

Auchtung

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Mechanistic investigation of host-microbe interactions in the human gut are hindered by difficulty of co-culturing microbes with intestinal epithelial cells. On one hand the gut bacteria are a mix of facultative, aerotolerant or obligate anaerobes, while the intestinal epithelium requires oxygen for growth and function. Thus, a coculture system that can recreate these contrasting oxygen requirements is critical step towards our understanding microbial-host interactions in the human gut. Here, we demonstrate Intestinal Organoid Physoxic Coculture (IOPC) system, a simple and cost-effective method for coculturing anaerobic intestinal bacteria with human intestinal organoids (HIOs). Using commensal anaerobes with varying degrees of oxygen tolerance, such as nano-aerobe Bacteroides thetaiotaomicron and strict anaerobe Blautia sp., we demonstrate that IOPC can successfully support 24–48 hours HIO-microbe coculture. The IOPC recapitulates the contrasting oxygen conditions across the intestinal epithelium seen in vivo. The IOPC cultured HIOs showed increased barrier integrity, and induced expression of immunomodulatory genes. A transcriptomic analysis suggests that HIOs from different donors show differences in the magnitude of their response to coculture with anaerobic bacteria. Thus, the IOPC system provides a robust coculture setup for investigating host-microbe interactions in complex, patient-derived intestinal tissues, that can facilitate the study of mechanisms underlying the role of the microbiome in health and disease.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction

Fofanova,

Karandikar,

Auchtung

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…An imbalance of intestinal microbiota is a crucial factor in the etiology, pathogenesis, and therapeutic management of a wide array of diseases. It is closely related to the modulation of the autoimmune system, drug metabolism, and therapeutic outcomes 21,22 . Among the myriad of metabolites produced by intestinal flora, SCFAs – consisting of organic fatty acids ranging from 1 to 6 carbon atoms – are some of the most essential markers 23,24 .…”

Section: Discussionmentioning

confidence: 99%

“…It is closely related to the modulation of the autoimmune system, drug metabolism, and therapeutic outcomes. 21,22 Among the myriad of metabolites produced by intestinal flora, SCFAsconsisting of organic fatty acids ranging from 1 to 6 carbon atomsare some of the most essential markers. 23,24 The depletion of SCFAs is a notable causative factor in spleen-qi deficiency.…”

Section: Discussionmentioning

confidence: 99%

Effects of ginseng on short‐chain fatty acids and intestinal microbiota in rats with spleen‐qi deficiency based on gas chromatography–mass spectrometry and 16s rRNA technology

Zhang,

Pi,

et al. 2023

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

RationaleSpleen‐qi deficiency syndrome, a common weakness syndrome in traditional Chinese medicine, results from insufficient spleen‐qi levels. For centuries, ginseng has been relied upon as a traditional Chinese medicine to treat spleen‐qi deficiency syndrome. Until now, the mechanism feature of ginseng in treating temper deficiency through intestinal bacteria and short‐chain fatty acid (SCFA) metabolites has not been fully elucidated.MethodsThis study established a rat model of spleen‐qi deficiency via multi‐factor compound modeling that involved fatigue injury and a controlled diet. The content of SCFAs between different treatment groups was determined by gas chromatography–mass spectrometry. And the 16s rRNA sequencing technology was applied to reveal the effects of ginseng on the intestinal microecological environment of the rats.ResultsIt was found that the ginseng treatment group exhibited the most remarkable regulatory effect on propionic acid, surpassing all other administration groups. Ginseng increased the relative abundance of beneficial bacteria and decreased that of harmful bacteria at the genus level in rats with spleen‐qi deficiency syndrome. And propionic acid is significantly positively correlated with Lactobacillus level and significantly negatively correlated with uncultured_bacterium_f_Muribaculaceae (p < 0.05). n‐Butyric acid is negatively correlated with the Faecalibaculum level (p < 0.01). n‐Valeric acid is significantly negatively correlated with the Romboutsia level (p < 0.01).ConclusionThe mechanism of ginseng treatment for spleen‐qi deficiency is elucidated from the perspective of gut microbiota and its metabolite SCFAs. It provides a new way for further development and utilization of ginseng and a theoretical basis.

show abstract

“…Existing studies have shown that ischemic stroke (IS) with dementia, depression, and other mental illness symptoms are common. The gut microbiome (GM) plays a critical role in mental illness and IS (42). However, this study aimed to identify the differences between genes related to ischemic stroke (IS) and mental disorders in the gut microbiome (GM) through bioinformatics analysis and qRT-PCR verification, and to predict drugs related to IS through candidate genes.…”

Section: Discussionmentioning

confidence: 99%

Predictive model, miRNA-TF network, related subgroup identification and drug prediction of ischemic stroke complicated with mental disorders based on genes related to gut microbiome

Shen

Yang

et al. 2023

Front. Neurol.

View full text Add to dashboard Cite

BackgroundPatients with comorbid schizophrenia, depression, drug use, and multiple psychiatric diagnoses have a greater risk of carotid revascularization following stroke. The gut microbiome (GM) plays a crucial role in the attack of mental illness and IS, which may become an index for the diagnosis of IS. A genomic study of the genetic commonalities between SC and IS, as well as its mediated pathways and immune infiltration, will be conducted to determine how schizophrenia contributes to the high prevalence of IS. According to our study, this could be an indicator of ischemic stroke development.MethodsWe selected two datasets of IS from the Gene Expression Omnibus (GEO), one for training and the other for the verification group. Five genes related to mental disorders and GM were extracted from Gene cards and other databases. Linear models for microarray data (Limma) analysis was utilized to identify differentially expressed genes (DEGs) and perform functional enrichment analysis. It was also used to conduct machine learning exercises such as random forest and regression to identify the best candidate for immune-related central genes. Protein–protein interaction (PPI) network and artificial neural network (ANN) were established for verification. The receiver operating characteristic (ROC) curve was drawn for the diagnosis of IS, and the diagnostic model was verified by qRT-PCR. Further immune cell infiltration analysis was performed to study the IS immune cell imbalance. We also performed consensus clustering (CC) to analyze the expression of candidate models under different subtypes. Finally, miRNA, transcription factors (TFs), and drugs related to candidate genes were collected through the Network analyst online platform.ResultsThrough comprehensive analysis, a diagnostic prediction model with good effect was obtained. Both the training group (AUC 0.82, CI 0.93–0.71) and the verification group (AUC 0.81, CI 0.90–0.72) had a good phenotype in the qRT-PCR test. And in verification group 2 we validated between the two groups with and without carotid-related ischemic cerebrovascular events (AUC 0.87, CI 1–0.64). Furthermore, we investigated cytokines in both GSEA and immune infiltration and verified cytokine-related responses by flow cytometry, particularly IL-6, which played an important role in IS occurrence and progression. Therefore, we speculate that mental illness may affect the development of IS in B cells and IL-6 in T cells. MiRNA (hsa-mir-129-2-3p, has-mir-335-5p, and has-mir-16-5p) and TFs (CREB1, FOXL1), which may be related to IS, were obtained.ConclusionThrough comprehensive analysis, a diagnostic prediction model with good effect was obtained. Both the training group (AUC 0.82, CI 0.93–0.71) and the verification group (AUC 0.81, CI 0.90–0.72) had a good phenotype in the qRT-PCR test. And in verification group 2 we validated between the two groups with and without carotid-related ischemic cerebrovascular events (AUC 0.87, CI 1–0.64). MiRNA (hsa-mir-129-2-3p, has-mir-335-5p, and has-mir-16-5p) and TFs (CREB1, FOXL1), which may be related to IS, were obtained.

show abstract

The mechanism of intestinal microbiota regulating immunity and inflammation in ischemic stroke and the role of natural botanical active ingredients in regulating intestinal microbiota: A review

Cited by 13 publications

References 202 publications

A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction

A novel system to culture human intestinal organoids under physiological oxygen content to study microbial-host interaction

Effects of ginseng on short‐chain fatty acids and intestinal microbiota in rats with spleen‐qi deficiency based on gas chromatography–mass spectrometry and 16s rRNA technology

Predictive model, miRNA-TF network, related subgroup identification and drug prediction of ischemic stroke complicated with mental disorders based on genes related to gut microbiome

Contact Info

Product

Resources

About