The mechanism of seed transmission of tobacco ringspot virus in soybean

Legume virus research in Canada: a retrospective and a view of the future

“…Similar effects on pollen germination and germ tube elongation were observed in AMV-infected alfalfa (Pesic & Hiruki 1988a), but pollen from alfalfa infected with a severe strain of AMV ) competed successfully with pollen from virus-free plants of certain clones to fertilize ovules (Pesic & Hiruki 1988a). Electron microscopy of pollen from TobRSV-infected soybean showed the presence of virus-like particles in the generative nuclei and the inner layer (intine) of the pollen wall (Yang & Hamilton 1974), suggesting a correlation between abnormal cytopathology of pollen, and poor germination and germ tube elongation.…”

“…TobRSV was detected by electron microscopy in egg cells of soybean, indicating that seed transmission was via infected eggs derived from infected megaspore mother cells (Yang & Hamilton 1974). In alfalfa, AMV enters the egg via infected pollen (Pesic et al 1988); viral antigen was detected by immunogold-labelling in the cytoplasm and vacuoles of integument cells, which later become the seed coat, but not in the embryo sac.…”

Changes induced by mycoplasma-like organisms (M.L.O.), etiologic agents of the Stolbur disease in the different tissues of the anther ofVinca roseaL. (Apocynaceae)

“…However, some cases of decreasing pollinic fertility because of the virus of the necrotic spots of fruit trees (hlarenaud & Saunier 1974). of Gertrniimt (Scarborough 8: Smith 1977, hlurdock et al 1976, the "Barley stripe mosaic" virus (Carroll & hlayhew 1976), the Tobacco ringspot virus in Soyabean (Yang & Hamilton 1974) are mentioned. But the phytopathological studies were oriented mainly to the localization of the particles inside the anther and not in response of the host-plant to the infection.…”

Conclusive Evidence of Replication of a Plant Virus in Honeybees Is Lacking

“…The genome sequences and the reverse transcription (RT)-PCR products reported by Li et al could have been generated from TRSV virions on the surface of, or inside, the honeybee or on pollen. As TRSV is pollen transmitted (4, 5), association of TRSV with honeybees via pollen is a distinct possibility.…”