The mechanism of the preventive effect of Shen’an capsule on the calcium oxalate crystal‐induced early renal injury based on metabolomics

Abstract

Kidney stone disease is a worldwide metabolism-associated disorder with a high incidence of renal dysfunction. However, effective methods to prevent crystalline nephropathy are still lacking owing to the absence of aetiological research. Shen'an (SA) capsules are prepared from Chinese medicinal compounds and are considered a promising treatment for the prevention of crystal-induced renal injury. In this study, 24 mice were randomly divided into four groups: saline, oxalate, SA-treated (via preventive administration) and SA-only groups. A metabolomics analysis based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was performed to explore the plasma metabolic profiles among the different groups. The amount of crystal deposition and the decline in kidney function were significantly alleviated by the use of SA capsule. A total of 24 metabolites that showed a reversal trend following SA capsule administration were identified as plasma biomarkerss of the preventive effects of SA capsules on crystal-induced renal injury. Most of these metabolites were involved in the metabolisms of lipid metabolism, energy metabolism, glutathione metabolism and vitamin metabolism. In conclusion, SA capsules exert a preventive effect in mice with crystal-induced kidney injury via the regulation of multiple metabolic pathways.

GC/MS-Based Metabolomic Analysis of A549 Cells Exposed to Emerging Organophosphate Flame Retardants

“…In the present study, the concentration of glycine (a precursor for the synthesis of GSH) decreased, suggesting that the cell’s antioxidant capacity had changed. Meanwhile, in the CDP-treated group, the concentration of pyroglutamate (a cyclized derivative of glutamate and a metabolite of the GSH cycle) declined [ 38 ], which may also suppress the GSH cycle and aggravate the oxidative damage to cells. It is also noteworthy that glutathione metabolism and the D-glutamine and D-glutamic acid metabolic pathways were disrupted in our study, as evidenced in Figure 5 A. Glutathione metabolism mainly plays a role in maintaining cell redox homeostasis [ 39 ], and the metabolic process of D-glutamine and D-glutamate has a protective antioxidant role [ 40 ].…”

Metabolic and Network Pharmacological Analyses of the Therapeutic Effect of Grona styracifolia on Calcium Oxalate-Induced Renal Injury

“…Metabolomics is a scientific study of the systemic metabolic changes in complex living systems in response to pathophysiological stimuli and has been effectively applied to reveal metabolic changes in diverse types of renal injury (Fan et al, 2018). In addition, the concept of network pharmacology has been developed due to the rapid development of bioinformatics.…”

Metabolomics study of fasudil on cisplatin-induced kidney injury

“…In this way, metabolomics is a powerful means of exploring potential biomarkers for diagnosis, treatment, and prognosis of various diseases [24–26]. Kidney injury is a complex pathophysiological process, and metabolomics has helped establish its pathological mechanism from the level of endogenous metabolites and has been used in many research applications [27,28]. In the present study, metabolic analysis of kidney tissues was performed to investigate changes in the metabolic regulatory network in a model of CDDP-induced kidney injury, and the biomarkers and pathophysiological mechanisms of CDDP-induced kidney injury were explored.…”