The mechanism of the tetrazolium reaction in identifying experimental myocardial infarction

Klein, Hermann H.; Puschmann, Stefan; Schaper, Jutta; Schäper, Wolfgang

doi:10.1007/bf00430828

Cited by 209 publications

(87 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the Merrick et al (1999) study, cytochrome oxidase reduced the TTC trifenilformazan (red dye), causing a measurable change in color. The inability to reduce the TTC was indicative of mitochondrial disruption and depletion of the oxidative phosphorylation enzymes, or both (Klein et al 1981). It was expected that the damage caused by the criolesion in this study was greater than that caused by the crushing injury promote by Merrick et al (1999) study, since the low temperature could alter the quaternary structure of enzymes (Alexandrov 1977).…”

Section: Effect Of Cryolesion In the Enzymatic Activity Of Injured Ratsmentioning

confidence: 73%

The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle

Oliveira

Durigan

Munin

et al. 2013

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

Section: Effect Of Cryolesion In the Enzymatic Activity Of Injured Ratsmentioning

confidence: 73%

The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle

Oliveira

Durigan

Munin

et al. 2013

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…However, infarct cells lost dehydrogenase enzyme and cofactor NADH and thus remained unstained or dull yellow. 35) The infarcted cells have lost the enzyme and cofactor and thus remained unstained or dull yellow. The ventricular slices were placed between two glass plates.…”

Section: Discussionmentioning

confidence: 99%

“…The extent of myocardial infarct size was estimated macroscopically by volume and weight method. 35) …”

Section: Assessment Of Myocardial Injurymentioning

confidence: 99%

Reduction of Ischemic, Pharmacological and Remote Preconditioning Effects by an Antioxidant N-Acetyl Cysteine Pretreatment in Isolated Rat Heart

et al. 2008

View full text Add to dashboard Cite

The present study was designed to investigate the possible role of free radicals in cardioprotective eŠects of ischemic, pharmacological and remote preconditioning. Isolated rat heart was perfused on LangendorŠ apparatus with Kreb's Henseleit solution and subjected to 30 min global ischemia followed by 120 min reperfusion. To assess myocardial injury, coronary eOEuent was analyzed for lactate dehydrogenase and creatine kinase activity. Myocardial infarct size was estimated using triphenyl tetrazolium chloride staining. Ischemic preconditioning, pharmacological preconditioning (angiotensin II; H 2 O 2 ), remote aortic preconditioning markedly attenuated I/R induced increase in lactate dehydrogenase and creatine kinase release and myocardial infarct size. Administration of N-Acetyl Cysteine (NAC), in vitro, during ischemic and pharmacological, and in vivo during remote preconditioning attenuated the cardioprotective eŠects of preconditioning. On the basis of these results, it may be concluded that sub threshold generation of Reactive Oxygen Species (ROS) may activate redox signaling which may be responsible for preconditioning induced cardioprotection.

show abstract

“…Although the validity of infarction size determined by TTC staining 6 hours after permanent MCAO has not been rigorously confirmed by electron microscopy, other studies have used TTC staining to determine necrosis within such a time frame. 25,34,35 Despite this limitation, TTC staining 6 hours after MCAO reveals that permanent mild hypothermia may significantly reduce infarction size. Zhang and coworkers 36 demonstrated that hypothermia is protective when induced 1 hour after transient ischemia in a focal stroke model.…”

Section: Discussionmentioning

confidence: 99%

Combination of Decompressive Craniectomy and Mild Hypothermia Ameliorates Infarction Volume After Permanent Focal Ischemia in Rats

Doerfler

Schwab²,

Hoffmann³

et al. 2001

Stroke

View full text Add to dashboard Cite

Background and Purpose-Both hypothermia and decompressive craniectomy (DC) have been shown to reduce ischemic injury in experimental middle cerebral artery (MCA) infarction. This study was designed to evaluate the effect of combined DC and hypothermia on infarction size and neurological outcome in a rat model of MCA occlusion (MCAO). Methods-MCAO was performed in 72 Wistar rats assigned to groups A through F. In group A, mild hypothermia (32°C) was induced 1 hour after MCAO for 5 hours; normothermia was maintained in group B. After 6 hours of survival, infarction size was calculated for animals of groups A and B. In group C, DC alone was performed 4 hours after MCAO; hypothermia without DC was performed 1 hour after MCAO and maintained for 5 hours in group D. Combined DC and hypothermia were performed in group E. No therapy was performed in group F (control). Infarction size and neurological performance after 24 hours were used as study end points (groups C through F). Results-Permanent

show abstract

The mechanism of the tetrazolium reaction in identifying experimental myocardial infarction

Cited by 209 publications

References 8 publications

The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle

The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle

Reduction of Ischemic, Pharmacological and Remote Preconditioning Effects by an Antioxidant N-Acetyl Cysteine Pretreatment in Isolated Rat Heart

Combination of Decompressive Craniectomy and Mild Hypothermia Ameliorates Infarction Volume After Permanent Focal Ischemia in Rats

Contact Info

Product

Resources

About