The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl Sulfate

Juan-Colás, José; Dresser, Lara; Morris, Katie; Lagadou, Hugo; Ward, Rebecca H.; Burns, Amy; Tear, S.P.; Johnson, Steven D.; Leake, Mark C.; Quinn, Steven D.

doi:10.1021/acs.langmuir.0c01810

Cited by 33 publications

(35 citation statements)

References 48 publications

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“…The size distribution of the prepared vesicles in buffer solution is evaluated by dynamic light scattering using a Zetasizer µV molecular size detector (Malvern Instruments Ltd, UK), whereby a mean polydispersity index <0.3 is observed [25,27]. When the vesicles are doped with 0.1 molar% of the fluorescent membrane stain 1,1 ′dioctadecyl-3,3,3 ′ ,3 ′ -tetramethylindocarbocyanine perchlorate, wide-field total internal reflection fluorescence microscopy (λ ex = 532 nm, λ em = 570 nm) is used to quantify the number and concentration of surface-immobilised vesicles per 25 × 50 µm field of view [27]. Scanning electron microscope (SEM) micrographs confirm the vesicles have an expected size of about 100 ± 20 nm, presenting a spherical and intact shape without fusing on the surface (figure 2(b)), confirming the possibility to use them as excellent model for exosomes.…”

Section: Detection Of Biotinylated Vesiclesmentioning

confidence: 99%

Dielectric metasurface for high-precision detection of large unilamellar vesicles

Conteduca

Quinn

Krauss

2021

J. Opt.

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Extracellular vesicles (EVs) are very promising biomarkers for the diagnosis of various diseases, including cardiovascular, infectious and neurodegenerative disorders. Of particular relevance is their importance in cancer liquid biopsy, where they play a key role in the early detection and monitoring of the tumour. A number of technologies have recently been developed to improve the performance of current EV detection methods, but a technique that can provide high resolution, high accuracy and a multiplexing capability for the detection of several biomarkers in parallel remains a challenge in this field. Here, we demonstrate the detection of large unilamellar vesicles, which are excellent models of EVs, down to a concentration <103 EV ml−1 (<10 pM) using a dielectric resonant metasurface. This result represents an improvement in performance and functionality compared to competing plasmonic and electrochemical modalities and is due to the strong resonance amplitude and high Q-factor of our metasurface. We also verify the selectivity of the approach by detecting vesicles that have been surface-functionalised with a CD9 protein. The ease of integration of our method into a point-of-care instrument offers a path towards personalised cancer medicine.

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Section: Detection Of Biotinylated Vesiclesmentioning

confidence: 99%

Dielectric metasurface for high-precision detection of large unilamellar vesicles

Conteduca

Quinn

Krauss

2021

J. Opt.

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“…Different studies have investigated the micelle-to-vesicle transition (MVT) process, by which mixed micelles transform into vesicles, and their effect on the reconstituted (or encapsulated) components onto liposomes, by describing the (molecular and supramolecular) out-of-equilibrium processes and providing quantitative information on the intermediate (unstable) aggregates, partition coefficients, etc. [ 72 , 73 , 74 , 75 , 76 , 77 ].…”

Section: Conventional Methods For the Preparation Of Liposomesmentioning

confidence: 99%

Methods of Liposomes Preparation: Formation and Control Factors of Versatile Nanocarriers for Biomedical and Nanomedicine Application

2022

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Liposomes are nano-sized spherical vesicles composed of an aqueous core surrounded by one (or more) phospholipid bilayer shells. Owing to their high biocompatibility, chemical composition variability, and ease of preparation, as well as their large variety of structural properties, liposomes have been employed in a large variety of nanomedicine and biomedical applications, including nanocarriers for drug delivery, in nutraceutical fields, for immunoassays, clinical diagnostics, tissue engineering, and theranostics formulations. Particularly important is the role of liposomes in drug-delivery applications, as they improve the performance of the encapsulated drugs, reducing side effects and toxicity by enhancing its in vitro- and in vivo-controlled delivery and activity. These applications stimulated a great effort for the scale-up of the formation processes in view of suitable industrial development. Despite the improvements of conventional approaches and the development of novel routes of liposome preparation, their intrinsic sensitivity to mechanical and chemical actions is responsible for some critical issues connected with a limited colloidal stability and reduced entrapment efficiency of cargo molecules. This article analyzes the main features of the formation and fabrication techniques of liposome nanocarriers, with a special focus on the structure, parameters, and the critical factors that influence the development of a suitable and stable formulation. Recent developments and new methods for liposome preparation are also discussed, with the objective of updating the reader and providing future directions for research and development.

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“…Preparation of vesicles incorporating the DiI and DiD FRET sensor. Large unilamellar vesicles composed of 0.1 mol % DiI, 0.1 mol % DiD, 1 % biotin-PE and 98.8 mol % POPC were prepared as previously described (23,24). Briefly, lipids, DiI and Did were mixed in chloroform before the solvent was evaporated.…”

Section: Methodsmentioning

confidence: 99%

Tween-20 induces the structural remodelling of single lipid vesicles

Dresser

Graham

Miller

et al. 2022

Preprint

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The interaction of Tween-20 with lipid membranes is crucial for a number of biotechnological applications including viral inactivation and membrane protein extraction, but the underlying mechanistic details have remained elusive. Evidence from ensemble assays supports a global model of Tween-20 induced membrane disruption that broadly encompasses association of the surfactant with the membrane surface, membrane fragmentation and the release of mixed micelles to solution, but whether this process involves intermediate and dynamic transitions between regimes is an open question. In search of the mechanistic origins of membrane disruption, increasing focus is put on identifying Tween-20 interactions with highly controllable model membranes. In light of this, and to unveil quantitative mechanistic details, we employed highly interdisciplinary biophysical approaches, including quartz-crystal microbalance with dissipation monitoring, steady-state and time-resolved fluorescence and FRET spectroscopy, dynamic light scattering, fluorescence correlation spectroscopy, wide-field single-vesicle imaging and scanning electron microscopy, to interrogate the interactions between Tween-20 and both freely-diffusing and surface-immobilized model-membrane vesicles. Using ultrasensitive sensing approaches, we discovered that Tween-20 leads to a stepwise and phase-dependent structural remodelling of sub-micron sized vesicles that includes permeabilization and swelling, even at detergent concentrations below the critical micellar concentration. These insights into the structural perturbation of lipid vesicles upon Tween-20 interaction highlight the impact on vesicle conformation prior to complete solubilization, and the tools presented may have general relevance for probing the interaction between lipid vesicles and a wide variety of disruptive agents.

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The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl Sulfate

Cited by 33 publications

References 48 publications

Dielectric metasurface for high-precision detection of large unilamellar vesicles

Dielectric metasurface for high-precision detection of large unilamellar vesicles

Methods of Liposomes Preparation: Formation and Control Factors of Versatile Nanocarriers for Biomedical and Nanomedicine Application

Tween-20 induces the structural remodelling of single lipid vesicles

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