The mechanisms of photoinhibition and repair in plants under high light conditions and interplay with abiotic stressors

Conserved proteins across oxygenic phototrophic organisms are important players in photosynthetic acclimation, yet many remain poorly or completely uncharacterized. In the green alga Chlamydomonas reinhardtii we identified Cre15.g636050, previously annotated as CPLD31, that encodes such an uncharacterized, conserved, thylakoid lumenal protein belonging to the "TPM" domain family (PF04536). TPM refers to a protein fold named after the three founding proteins of this family, TLP18.3, Psb32, and MOLO-1, with the first two being Photosystem II-related proteins. We also found a paralogue of CPLD31, Cre03.g154600, previously annotated as TLP15.2. In this work, we generated knock-out mutants of these genes in Chlamydomonas and produced the recombinant proteins. We showed both proteins are regulated by light and phototrophy, and while the former binds manganese in a TPM-domain metal binding site, the latter does not. Therefore, we reannotated them as Lumenal Mn-binding TPM-domain Protein 1 (LMTP1) and Thylakoid Lumenal Protein 26 kDa (TLP26), respectively.

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Comparative Plastomics of Plantains (Plantago, Plantaginaceae) as a Tool for the Development of Species-Specific DNA Barcodes

Mehmood,

Li,

Bertolli

et al. 2024

Plants

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Plantago (plantains, Plantaginaceae) is a cosmopolitan genus including over 250 species used as functional foods, forage, and traditional medicine. Among them, Plantago lanceolata is commonly used as an ingredient of herbal products, but the close similarity to other Plantago species can cause misidentifications with potentially serious consequences for product safety/quality. To test the possibility of developing species-specific barcoding markers, we de novo assembled plastome sequences of individuals of Plantago argentea, Plantago atrata, P. lanceolata, and Plantago maritima. These genomes were characterized in comparison with both previously sequenced conspecific accessions and other publicly available plastomes, thus providing an assessment of both intraspecific and interspecific genetic variation in Plantago plastomes. Additionally, molecular evolutionary analyses indicated that eleven protein-coding genes involved in different plastid functions in Plantago plastomes underwent positive selection, suggesting they might have contributed to enhancing species’ adaptation during the evolutionary history of Plantago. While the most variable mutational hotspots in Plantago plastomes were not suitable for the development of species-specific molecular markers, species-specific polymorphisms could discriminate P. lanceolata from its closest relatives. Taken together, these results highlight the potential of plastome sequencing for the development of molecular markers to improve the identification of species with relevance in herbal products.

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The mechanisms of photoinhibition and repair in plants under high light conditions and interplay with abiotic stressors

Cited by 3 publications

References 142 publications

Expression of the LlBANMT Gene from Sea Lavender Leads to the Accumulation of β-alanine Betaine and Enhanced Stress Resilience in Transgenic Tobacco Plants

Expression of the LlBANMT Gene from Sea Lavender Leads to the Accumulation of β-alanine Betaine and Enhanced Stress Resilience in Transgenic Tobacco Plants

Crystal structure of a Photosystem II-related, Manganese-binding, TPM-domain protein at a 1.6 Å resolution

Comparative Plastomics of Plantains (Plantago, Plantaginaceae) as a Tool for the Development of Species-Specific DNA Barcodes

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