2015
DOI: 10.1016/j.bbamem.2014.10.022
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The mechanosensitive channel of small conductance (MscS) functions as a Jack-In-The Box

Abstract: Phenotypical analysis of the lipid interacting residues in the closed state of the mechanosensitive channel of small conductance (MscS) from Escherichia coli (E. coli) has previously shown that these residues are critical for channel function. In the closed state, mutation of individual hydrophobic lipid lining residues to alanine, thus reducing the hydrophobicity, resulted in phenotypic changes that were observable using in vivo assays. Here, in an analogous set of experiments, we identify eleven residues in … Show more

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Cited by 32 publications
(23 citation statements)
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“…This extension, including its additional transmembrane domain, is responsible for the unusual gating mechanism of MscCG (Nakayama et al 2016) with a lower activation threshold and slow closing. In contrast, canonical MscS channels gate in response to hypoosmotic shock and seemingly follow the Jack-in-the-box mechanism (Malcolm et al 2015), which does not fit for MscCG as a consequence of structural adaptations (Nakayama et al 2019(Nakayama et al , 2018a.…”
Section: Discussionmentioning
confidence: 95%
“…This extension, including its additional transmembrane domain, is responsible for the unusual gating mechanism of MscCG (Nakayama et al 2016) with a lower activation threshold and slow closing. In contrast, canonical MscS channels gate in response to hypoosmotic shock and seemingly follow the Jack-in-the-box mechanism (Malcolm et al 2015), which does not fit for MscCG as a consequence of structural adaptations (Nakayama et al 2019(Nakayama et al , 2018a.…”
Section: Discussionmentioning
confidence: 95%
“…Ng-MscS shows typical MscS-like electrophysiological properties. To characterize the activity of Ng-MscS, the electrophysiological properties of Ng-MscS were determined and compared with those of Ec-MscS by patch clamp analyses of E. coli spheroplasts (23,24). Membrane patches containing Ng-MscS or Ec-MscS were exposed to a gradual increase in negative pressure.…”
Section: Resultsmentioning
confidence: 99%
“…Preparation of spheroplasts. Preparation of E. coli spheroplasts of MJF465 and MJF431 containing vectors pB10b-Ng-mscS and pB10b-Ec-mscS was performed according to previously described procedures (23). Single colonies were used to inoculate modified LB broth containing 0.5% (wt/vol) NaCl (Vetec) and ampicillin and grown overnight at 37°C with shaking.…”
Section: Methodsmentioning
confidence: 99%
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“…Several hydrophobic residues interact with membrane lipids in the closed state but are embedded in the protein core in the open state. Moreover, mutating residues that interact with membrane lipids in the open state did not alter the channel gating, which lead the authors to propose the Jack-in-the-box model [27]. In this model, interactions between hydrophobic residues and membrane lipids would maintain the channel in the closed state while an increase in membrane tension would destabilize these interactions to promote the open state.…”
Section: Activation By Tensionmentioning
confidence: 99%