The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats

Sotoudeh, Narges; Namavar, Mohammad Reza; Bagheri, Farshid; Zarifkar, Asadollah

doi:10.1002/brb3.2616

Cited by 12 publications

(5 citation statements)

References 69 publications

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“…Exploratory activity, autonomous animal, and anxiety-like behavior were examined by using an open-field apparatus (100 × 100 × 100 cm) [ 44 , 45 ]. The center area was set as a square 20 cm away from the wall of the open-field apparatus.…”

Section: Methodsmentioning

confidence: 99%

PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders

Wang

Han

et al. 2022

Cell Biosci

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Background Clinical and animal studies demonstrated that neuroinflammation from anesthesia (sevoflurane) is the main contributor to cause perioperative neurocognitive disorders (PND). Recently, it was reported that microglia respond to hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which was the target of sevoflurane. Whether HCN channels are involved in the induction of neuroinflammation after sevoflurane exposure is still unclear. Results Sevoflurane exposure had increased cognitive dysfunction and anxiety-like behaviors in rats. Rats inhaled with sevoflurane had activated microglia and increased neuroinflammation (IL-1β, IL-6, and TNF-α) in the hippocampus. RNA sequencing identified 132 DEGs (86 up-regulated and 46 down-regulated DEGs [differentially expressed genes]) in the hippocampus of PND rats. RNA-sequencing also uncovered that sevoflurane exposure down-regulates HCN2 expression. Pathway and process enrichment analysis suggests DEGs are mainly enriched in regulation of system process, positive regulation of glutamate secretion, secretion, regulation of synaptic transmission, regulation of nervous system process, behavior, negative regulation of sodium ion transport, and learning or memory. We validated that sevoflurane exposure can down-regulate the levels of PEX5R/Trip8b (an interaction partner and auxiliary subunit of HCN channels) and HCN1-4 channels in the hippocampus of PND rats. We used immunofluorescence staining to identify that HCN2 co-labels with neurons (Neun), astrocytes (GFAP), and microglia (iba1). We observed that the co-labeling of HCN2 with neurons or microglia decreased in the hippocampus and cortex after sevoflurane exposure. Blocking HCN2 by ZD7288 treatment further activated microglia and aggravated sevoflurane exposure-induced anxiety-like behavior, cognitive impairment, and neuroinflammation. Conclusions We concluded that sevoflurane exposure can induce an increased level of neuroinflammation, microglial activation, cognitive dysfunction, and anxiety-like behaviors in rats. HCN2 channel, as the target of sevoflurane action, mediates this process. HCN2 might be a target for the treatment and prevention of sevoflurane-induced PND.

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Section: Methodsmentioning

confidence: 99%

PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders

Wang

Han

et al. 2022

Cell Biosci

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“…The animal is then placed in the centre of the cube for 10 minutes, and its activity is recorded. The software measures and analyzes the total time spent in the centre zone (in seconds), time spent in the peripheral zone (in seconds), and total travelled distance (in centimetres) over 10 minutes (Sotoudeh et al, 2022).…”

Section: Locomotor Evaluationmentioning

confidence: 99%

Berberine Chloride Mitigates Cerebellar Damage in Global Cerebral Ischemia: A Comprehensive Study with stereological analysis, evaluation the Antioxidant response and Locomotor Function in Rat Models

Mehboodi,

Shahedi,

Namavar

et al. 2024

Preprint

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Global cerebral ischemia (GCI) leads to significant oxidative damage in the cerebellum, which mainly affects Purkinje cells. This study aimed to investigate the neuroprotective effects of Berberine chloride (BBR), a compound known for its antioxidant properties against GCI. 42 adult male Wistar rats were divided into three groups: Sham, GCI, and GCI+BBR. Rats received BBR (50mg/kg) 7 days before and 6 hours after inducing GCI via bilateral common carotid artery occlusion for 20 minutes. And assessed for locomotor activity by open field test, cerebellar biochemical factors malondialdehyde (MDA), superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), cerebellum volume and Purkinje neuron count by stereological analysis.The BBR treatment reduced the concentration of MDA(P<0.0001) and increased the activity of antioxidant enzymes SOD, CAT and GPx (P<0.0001, P<0.001 and P<0.001, respectively) in the cerebellum compared to the GCI group. Stereological analysis revealed higher Purkinje cell count (P<0.0001), cerebellum (P<0.001), white matter (P<0.01), and grey matter (P<0.0001) volume in the GCI+BBR group compared to the GCI group. Furthermore, GCI+BBR showed enhanced locomotor function compared to the GCI group. BBR showed therapeutic benefits and improved locomotor function, Showcased antioxidative effects by lowering MDA levels, boosting enzymatic activities, and significantly mitigating Purkinje cell death and cerebellar volume loss.

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“…Through experimentation, it was discovered that FG’s two-photon excitation spectrum ranges from 720 to 990 nm. Among the wavelengths within the microscope’s tuning range, 720 nm is the ideal two-photon excitation wavelength, while 760 nm is the highest lifetime contrast wavelength ( Miller et al, 2021 ; Sotoudeh et al, 2022 ). The broad fluorescence emission spectrum of FG often results in spectral overlap with other fluorophores, which can impede multicolor imaging.…”

Section: Neuro-nonspecific Agentsmentioning

confidence: 99%

Advances in optical molecular imaging for neural visualization

Wei,

Liu,

Liang

et al. 2023

Front. Bioeng. Biotechnol.

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Iatrogenic nerve injury is a significant complication in surgery, which can negatively impact patients’ quality of life. Currently, the main clinical neuroimaging methods, such as computed tomography, magnetic resonance imaging, and high-resolution ultrasonography, do not offer precise real-time positioning images for doctors during surgery. The clinical application of optical molecular imaging technology has led to the emergence of new concepts such as optical molecular imaging surgery, targeted surgery, and molecular-guided surgery. These advancements have made it possible to directly visualize surgical target areas, thereby providing a novel method for real-time identification of nerves during surgery planning. Unlike traditional white light imaging, optical molecular imaging technology enables precise positioning and identifies the cation of intraoperative nerves through the presentation of color images. Although a large number of experiments and data support its development, there are few reports on its actual clinical application. This paper summarizes the research results of optical molecular imaging technology and its ability to realize neural visualization. Additionally, it discusses the challenges neural visualization recognition faces and future development opportunities.

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The medial prefrontal cortex to the medial amygdala connections may affect the anxiety level in aged rats

Cited by 12 publications

References 69 publications

PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders

PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders

Berberine Chloride Mitigates Cerebellar Damage in Global Cerebral Ischemia: A Comprehensive Study with stereological analysis, evaluation the Antioxidant response and Locomotor Function in Rat Models

Advances in optical molecular imaging for neural visualization

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