The purposes of this research were: (1) to study strategic human resource management Thai Universities; (2) to study mission implementation of Thai Universities; (3) to find a relationship between strategic human resource management and mission implementation of Thai Universities; and (4) to analyze the influence of strategic human resource management on mission implementation of Thai Universities. This study applied a quantitative method. The samples used in this study were 350 personnel of Thai Universities located in Bangkok. The research questionnaire was used as a tool to collect data. Statistics used in this study consisted of percentage, mean, standard deviation, Product-Moment Correlation Coefficient and Multiple Regression Analysis (MRA). The research results showed that (1) Strategic human resource management of Thai Universities, in total, was perceived at high level. In particular, human resource policy and planning had the highest mean value, followed by knowledge and skill, benefits and compensation, recruitment and selection and employee retention respectively. (2) Mission implementation of Thai Universities, in total, was perceived at high level. In particular, graduate development had the highest mean value, followed by academic service, cultural conservation and research conduct respectively. (3) Strategic human resource management was positively related to mission implementation of Thai Universities. (4) Strategic human resource management had an influence on mission implementation of Thai Universities. In particular, human resource policy and planning had the highest influence on mission implementation of Thai Universities, followed by knowledge and skill, benefits and compensation, recruitment and selection and employee retention. This study recommended that Thai Universities should apply strategic human resource management in linkage with organizational policy to extract talent, skill, knowledge, expertise and specialization of employees to work for the fulfillment of mission implementation of the universities.