The medical leech: An old treatment revisited

Wells, Mark D.; Manktelow, Ralph T.; Boyd, J. Brian; Bowen, Vaughan T.

doi:10.1002/micr.1920140309

Cited by 72 publications

(51 citation statements)

References 17 publications

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“…50,51 Due to its relative accessibility and simplicity, the CNS of the medicinal leech has been extensively studied. The CNS of the leech consists of thirty-two bilateral neuromeres, of which the 4 anterior-most fuse to form the sub-esophageal ganglion and the 7 posterior-most fuse to form the caudal ganglion.…”

Section: Resultsmentioning

confidence: 99%

Top-Down Atmospheric Ionization Mass Spectrometry Microscopy Combined With Proteogenomics

Hsu

Baker²,

Gaasterland³

et al. 2017

Anal. Chem.

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Mass spectrometry-based protein analysis has become an important methodology for proteogenomic mapping by providing evidence for the existence of proteins predicted at the genomic level. However, screening and identification of proteins directly on tissue samples, where histological information is preserved, remain challenging. Here we demonstrate that the ambient ionization source, nanospray desorption electrospray ionization (nanoDESI), interfaced with light microscopy allows for protein profiling directly on animal tissues at the microscopic scale. Peptide fragments for mass spectrometry analysis were obtained directly on ganglia of the medicinal leech (Hirudo medicinalis) without in-gel digestion. We found that a hypothetical protein, which is predicted by the leech genome, is highly expressed on the specialized neural cells that are uniquely found in adult sex segmental ganglia. Via this top-down analysis, a posttranslational modification (PTM) of tyrosine sulfation to this neuropeptide was resolved. This three-in-one platform, including mass spectrometry, microscopy, and genome mining, provides an effective way for mappings of proteomes under the lens of a light microscope.

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Section: Resultsmentioning

confidence: 99%

Top-Down Atmospheric Ionization Mass Spectrometry Microscopy Combined With Proteogenomics

Hsu

Baker²,

Gaasterland³

et al. 2017

Anal. Chem.

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“…20 Otra de la posibles complicaciones es la anemización severa del paciente, debida a una hemorragia contínua. El volumen de sangre perdido depende del número de sanguijuelas utilizadas y de la frecuencia y tiempo total de aplicación, 21 pero no es en ningún caso despreciable, por lo que es práctica obligada monitorizar las constantes del paciente así como la hemoglobina y el hematocrito durante el tratamiento con sanguijuelas.…”

Section: Discussionunclassified

Rescate con sanguijuelas de un injerto libre lateral del muslo

Morán¹,

Montesdeoca²,

Burgueño³

et al. 2003

Rev Esp Cirug Oral y Maxilofac

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A pesar de que Humphrey Bogart llamó a las sanguijuelas asquerosos diablillos en "la Reina de África", los cirujanos de hoy en día, rodeados de técnicas sofisticadas, tienen que recurrir con frecuencia a estos ané-lidos o afrontar la pérdida de un injerto.Las sanguijuelas se usaron como medio de practicar sangrías durante más de 2000 años, alcanzando su época de máximo esplendor en el s.XVIII y primera mitad del s. XIX, momento en el cual llegaron a ser una especie en peligro de extinción en Europa. 1 Con el advenimiento de la medicina científica en la segunda mitad del s.XIX, las sangrías dejaron de practicarse y las sanguijuelas cayeron en desuso.Actualmente, en la medicina moderna, se utilizan en cirugía reconstructiva para aliviar la congestión venosa. [2][3][4][5][6] Este resurgir es contínuo desde mediados de los setenta, cuando un grupo de cirujanos franceses pioneros en cirugía reconstructiva demostraron su utilidad en la supervivencia de colgajos y en los reimplantes digitales. 7,8 Caso clínico Abstract:The overall success rates using free flaps is nowadays over 95% in the majority of services. Salvage attempts successfully rescue from 28-87,5% of the cases in different series. Venous insufficienency is responsible for most initial flap failures We report a case in which a venous trombosis of the free flap was detected and it was finally successfully rescued with leeches.

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“…[26][27][28][29][30] The most common leech used is Hirudo medicinalis. The use of medicinal leeches can increase the risk of wound infections.…”

Section: Leechesmentioning

confidence: 99%