The Meiotic Bouquet Promotes Homolog Interactions and Restricts Ectopic Recombination in<i>Schizosaccharomyces pombe</i>

Davis, Luther; Smith, Gerald R.

doi:10.1534/genetics.106.059733

Cited by 43 publications

(52 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…47). Finally, telomere movements could contribute to the dissolution of nonhomologous associations such as those between heterologous centromeres (48) and other ectopic interactions (18,39), essentially editing out connections that might hinder rather than promote appropriate disjunction in the first meiotic division (49). The role played by transient accumulation of telomeres adjacent to the spindle pole, specifically, remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…These markers were integrated in the middle of the left arm of chromosome VII, at the left telomere of chromosome III or at the right telomere of chromosome IV, positions representing an interstitial site (VII) and telomere-proximal sites of short (III) and long (IV) chromosome arms. In strains with homozygous spots, the numbers of distinct fluorescent spots indicate whether the marked chromosome regions are unpaired (2 spots) or paired (1 spot) (39,40). An interstitial locus on VIIL was paired in 40-50% of wild-type cells upon entry into sporulation (Fig.…”

Section: Ndj1p and Mps3pmentioning

confidence: 99%

See 1 more Smart Citation

MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae

Conrad

Lee

Wilkerson

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

139

254

View full text Add to dashboard Cite

In meiotic prophase, telomeres associate with the nuclear envelope and accumulate adjacent to the centrosome/spindle pole to form the chromosome bouquet, a well conserved event that in Saccharomyces cerevisiae requires the meiotic telomere protein Ndj1p. Ndj1p interacts with Mps3p, a nuclear envelope SUN domain protein that is required for spindle pole body duplication and for sister chromatid cohesion. Removal of the Ndj1p-interaction domain from MPS3 creates an ndj1⌬-like separation-of-function allele, and Ndj1p and Mps3p are codependent for stable association with the telomeres. SUN domain proteins are found in the nuclear envelope across phyla and are implicated in mediating interactions between the interior of the nucleus and the cytoskeleton. Our observations indicate a general mechanism for meiotic telomere movements.meiosis ͉ SUN ͉ telomere ͉ nondisjunction H omologous chromosome pairing and recombination during meiotic prophase are required to orient chromosomes for disjunction and haploidization during the meiotic divisions. Early in meiotic prophase, telomeres attach to and move along the nuclear envelope to concentrate transiently in one sector of the nucleus, generally adjacent to the centrosome, forming the chromosome bouquet, a widely conserved arrangement (1-7). Bouquet formation may promote homologous chromosome pairing and also may help to untangle chromosomes, to regulate recombination at telomeres, to regulate crossover distribution, to coordinate or synchronize chromosomal events by propagating signals from the telomeres, and/or to facilitate synaptonemal complex formation (see refs. 1, 5, and 7-10). Despite the wide conservation of bouquet formation, the molecular mechanisms responsible for telomere attachments and movements in meiosis are poorly understood.In Saccharomyces cerevisiae, the meiotic bouquet resembles that of multicellular organisms (11)(12)(13)(14). Ndj1p is a meiosisspecific protein that accumulates at telomeres in S. cerevisiae (15,16) and is required for bouquet formation (17). Deletion of NDJ1 delays axial element formation, homolog pairing, synapsis and onset of the first meiotic division, causes an elevated frequency of nondisjunction and of ectopic recombination, and reduces spore formation and viability (15-21). Early recombination intermediates form between homologs with wild-type kinetics in ndj1⌬, suggesting that some aspect of bouquet formation is required for the normal coupling of the molecular and cytological events of pairing (22).A large-scale two-hybrid screen (23) identified an interaction between NDJ1 and MPS3/NEP98 (24, 25). Mps3p is an essential, integral membrane protein that is concentrated at the spindle pole body (SPB), the S. cerevisiae centrosome equivalent, and is present at lower levels throughout the nuclear membrane. Mps3p is required for duplication of the SPB (24, 25) and also functions in karyogamy and in sister chromatid cohesion (25,26). The present study demonstrates a critical requirement for the Ndj1p-Mps3p interaction for bouquet forma...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Ndj1p and Mps3pmentioning

confidence: 99%

MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae

Conrad

Lee

Wilkerson

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

139

254

View full text Add to dashboard Cite

show abstract

“…Appropriately diluted mitotic cultures of the ade6-Dupcontaining strain were plated on YEA 1 adenine to determine the total number of viable cells and on YEA 1 guanine to determine the frequency of mitotic Ade 1 recombinants (Schuchert and Kohli 1988;Davis and Smith 2006). The ade6-Dup strain and the appropriate ade6-D19 (complete ade6 deletion) strain were then mated on supplemented SPA.…”

Section: Yeast Strains and Genetic Techniquesmentioning

confidence: 99%

The Fission Yeast BLM Homolog Rqh1 Promotes Meiotic Recombination

2008

Self Cite

View full text Add to dashboard Cite

RecQ helicases are found in organisms as diverse as bacteria, fungi, and mammals. These proteins promote genome stability, and mutations affecting human RecQ proteins underlie premature aging and cancer predisposition syndromes, including Bloom syndrome, caused by mutations affecting the BLM protein. In this study we show that mutants lacking the Rqh1 protein of the fission yeast Schizosaccharomyces pombe, a RecQ and BLM homolog, have substantially reduced meiotic recombination, both gene conversions and crossovers. The relative proportion of gene conversions having associated crossovers is unchanged from that in wild type. In rqh1 mutants, meiotic DNA double-strand breaks are formed and disappear with wild-type frequency and kinetics, and spore viability is only moderately reduced. Genetic analyses and the wild-type frequency of both intersister and interhomolog joint molecules argue against these phenotypes being explained by an increase in intersister recombination at the expense of interhomolog recombination. We suggest that Rqh1 extends hybrid DNA and biases the recombination outcome toward crossing over. Our results contrast dramatically with those from the budding yeast ortholog, Sgs1, which has a meiotic antirecombination function that suppresses recombination events involving more than two DNA duplexes. These observations underscore the multiple recombination functions of RecQ homologs and emphasize that even conserved proteins can be adapted to play different roles in different organisms.

show abstract

“…Since telomeres have been shown to cluster together in Sz. pombe (Chikashige et al, 2006;Davis and Smith, 2006;Tang et al, 2006), which promotes homologue interactions and inhibits ectopic recombination in wild-type strains (Chikashige et al, 2006;Davis and Smith, 2006;Tang et al, 2006), possibilities of inter-and intrachromosomal recombination occurring is likely in the mutants. Notably, clr1, clr3, clr4 and swi6 mutants also share a common band of 1.3 kb ( Figure 4C), which may originate from a recombination event at a common site in the subtelomeric region.…”

Section: Subtelomeric Recombination In Heterochromatin Mutantsmentioning

confidence: 99%

Role of heterochromatin in suppressing subtelomeric recombination in fission yeast

Bisht

Arora

Ahmed

et al. 2008

Yeast

View full text Add to dashboard Cite

Telomere length is regulated by a complex interplay of several factors, including telomerase, telomere-binding proteins, DNA replication machinery and recombination. In yeast, DNA polymerase α is required for de novo synthesis of telomeres from broken ends of DNA, and it also suppresses the elongation of normal telomeric repeats. Heterochromatin proteins Clr1-Clr4 and Swi6 and DNA polα organize heterochromatin structure at mating type, centromere, rDNA and telomere regions that are refractory to transcription and recombination in Schizosaccharomyces pombe. Here, we have addressed the role of heterochromatin structure in regulating the integrity and organization of telomeric regions. Here, we show that subtelomeric duplication and rearrangements occur in polα and heterochromatin mutants and find that some of the putative duplication events are dependent on the Rad50 pathway. Thus, our study shows a role of heterochromatin in maintaining the integrity of the subtelomeric regions by suppressing their recombination in Sz. pombe.

show abstract

The Meiotic Bouquet Promotes Homolog Interactions and Restricts Ectopic Recombination inSchizosaccharomyces pombe

Cited by 43 publications

References 95 publications

MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae

MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae

The Fission Yeast BLM Homolog Rqh1 Promotes Meiotic Recombination

Role of heterochromatin in suppressing subtelomeric recombination in fission yeast

Contact Info

Product

Resources

About