The Melanoma Antigen Gene (MAGE) Family Is Clustered in the Chromosomal Band Xq28

Rogner, Ute Christine; Wilke, Klaus; Steck, Eric; Korn, Bernhard; Poustka, Annemarie

doi:10.1006/geno.1995.9945

Cited by 101 publications

(89 citation statements)

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“…These included a human exon from a melanoma antigen gene (MAGE) family member and two exons from a murine cosrnid that demonstrated identity or near-identity with sequence from the Xlr3 subfamily of X-linked, murine-specific lymphocyte-regulated genes. Independent mapping of human MAGE family members within Xq28 (Rogner et al 1995) and of the two highly homologous murine genes Xlr3a and XlrJb near Bgn on the mouse X chromosome (Bergsagel et al 1994), are consistent with our exon trapping data. A full-length Xlr3b cDNA probe was obtained from Dr. Leif Bergsagel (National Cancer Institute, Bethesda, MD); it demonstrates a complex pattern of hybridization to the YACs B16S5, B20S1, C131B2, and D35D6 as well as to selected murine cosmids (data not shown).…”

Section: Exon Isolation and Characterizationsupporting

confidence: 88%

“…1. The position of the MAGE cDNA was assigned solely on the basis of the trapped exon and mapping performed by Rogner et al (1995).…”

Section: Cosmid Contig Constructionmentioning

confidence: 99%

“…In the LIM domain, 52 of 56 amino acids are identical (93%) and the four differences are conservative substitutions. Rogner et al (1995) this work--hybridization screening of cDNA library using exons 10-27, 10-41, and 10-46 a88-bp exon fragment LC50 with 240-bp partial cDNA.…”

Section: Pi-amentioning

confidence: 99%

“…In the equivalent human interval, there are also repeated elements that include the transcribed MAGE genes (Rogner et al 1995) and three copies of the sequence DXS52 (Feil et al 1990). The functional significance of these repeats is unknown, but similar clusters of repeats have been identified in several other regions of the X chromosome and many autosomes.…”

Section: T T E D Y K K L a P Y N I R Rmentioning

confidence: 99%

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A comparative transcription map of the murine bare patches (Bpa) and striated (Str) critical regions and human Xq28.

Levin¹,

Chatterjee²,

Pragliola³

et al. 1996

Genome Res.

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The X-linked developmental mouse mutations bare patches (Bpa) and striated {Str) may be homologous to human X-linked dominant chondrodysplasia punctata (CDPX2) and incontinentia pigmenti (IP2), respectively, based on their genetic mapping and clinical phenotypes. Bpa and Str have been localized to an overlapping critical region of 600 kb that demonstrates conserved gene order with loci in human Xq28 between DXSII04 and DXS52. As part of efforts to isolate the genes involved in these disorders, we have begun to develop a comparative transcription map spanning this region in both species. Using techniques of cross-species conservation and hybridization, exon trapping, and cDNA selection we have identified four known genes or members of gene families--caltractin, a member of the ~/-aminobutyric acid (GABAA) receptor gene family, a member of the melanoma antigen gene (MAGE) family, and several members of the murine-specific, X-linked lymphocyte regulated gene (Xlr3) family. Trapped exons and, in some cases, longer cDNAs have been isolated for potentially 7-9 additional genes. One cDNA demonstrates highly significant homology with members of the Kriippel family of zinc finger transcription factors. A second novel cDNA demonstrates homology at the 3' end of the predicted amino acid sequence to a LIM domain consensus. Gene order appears conserved among those cDNAs determined to be present in both human and mouse. Three of the murine transcripts appear to be present in multiple copies within the Bpa/Str critical region and could be associated with a predisposition to genomic rearrangements. Reverse trancriptase PCR (RT-PCR) and Northern analyses demonstrate that several of the transcripts are expressed in mid-gestation murine embryos and neonatal skin, making them candidates for the Bpa and Str mutations and their respective homologous human disorders.

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Section: Exon Isolation and Characterizationsupporting

confidence: 88%

“…1. The position of the MAGE cDNA was assigned solely on the basis of the trapped exon and mapping performed by Rogner et al (1995).…”

Section: Cosmid Contig Constructionmentioning

confidence: 99%

Section: Pi-amentioning

confidence: 99%

Section: T T E D Y K K L a P Y N I R Rmentioning

confidence: 99%

See 2 more Smart Citations

A comparative transcription map of the murine bare patches (Bpa) and striated (Str) critical regions and human Xq28.

Levin¹,

Chatterjee²,

Pragliola³

et al. 1996

Genome Res.

View full text Add to dashboard Cite

show abstract

“…The MAGE-A family consists of 12 subtypes including MAGE-A1 to -A12 [7][8][9][10]. Based on the reverse transcriptase polymerase chain reaction (RT-PCR) typing results for MAGE-A genes, except MAGE-A7 which is not found to be not transcribed, MAGE-A is expressed almost exclusively in various tumor types and testis [9][10][11][12][13][14][15][16][17][18][19]. Expression of single MAGE-A genes has already been extensively examined for the diagnostic application of different malignancies.…”

Section: Introductionmentioning

confidence: 99%

A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

et al. 2009

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Abstract. Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC). Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples. Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages. Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.

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Expression of MAGE-1, MAGE-3 and MART-1 genes in neuroblastoma

et al. 1996

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The human MAGE-1, MAGE-3 and MART-1 genes code for antigens that are specifically recognized by cytolytic T lymphocytes in a MHC-restricted manner. The MAGE-1 and MAGE-3 genes are expressed in tumors of different histotypes but not in normal adult tissues (with the exception of testis), while the MART-1 gene appears to be selectively expressed in melanoma. MAGE-1, MAGE-3 and MART-1 antigens may therefore constitute useful targets for specific anti-tumor immunization of cancer patients. Here we have investigated the expression of MAGE-1, MAGE-3 and MART-1 in 11 neuroblastoma (NB) cell lines and 73 NB tumor masses. MAGE-1 and MAGE-3 transcripts were detected simultaneously in 36% of the cell lines and in 16% of tumor samples. The MAGE-1 gene was never expressed alone except in one tumor. In contrast, MAGE-3 mRNA was found in approximately 40% of the NB tumor samples in the absence of MAGE-1 mRNA. No expression of the MART-1 gene was observed in any cell line or tumor sample. No correlation was found between MAGE gene expression and clinical stage, event-free survival and presence or absence of N-myc amplification.

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The Melanoma Antigen Gene (MAGE) Family Is Clustered in the Chromosomal Band Xq28

Cited by 101 publications

References 0 publications

A comparative transcription map of the murine bare patches (Bpa) and striated (Str) critical regions and human Xq28.

A comparative transcription map of the murine bare patches (Bpa) and striated (Str) critical regions and human Xq28.

A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

Expression of MAGE-1, MAGE-3 and MART-1 genes in neuroblastoma

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