The Membrane Properties, and Excitationcontraction Coupling of M. Digastricus of the Guinea Pig

Ohbu, Teruyoshi

doi:10.2170/jjphysiol.22.205

Cited by 4 publications

(6 citation statements)

References 22 publications

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“…It has been reported by OHBU (1972) that an electron microscopic observation of the individual muscle fibres in M. digastricus showed nearly the same structures, and Z-line located between the sarcomeres was straight and smooth. However, the electrical properties of the membrane could be classified into two muscle fibre types, i.e.…”

Section: Discussionmentioning

confidence: 73%

“…Effects of caffeine and thymol on the twitch tension development were preliminary reported by OHBU (1972). In the present experiments, more detailed investigations were carried out.…”

Section: Effects Of Caffeine and Thymol On The Mechanical Responsementioning

confidence: 72%

“…However, both muscle fibre types evoked the action potential with overshoot and produced fast twitch tension development. This might indicate that at least two different types of the muscle fibre are distributed in the tissue (OHBU, 1972). ii) M. flexor hallucis longus of guinea pig is mainly composed of the white fibre (72.8%) and M. vastus lateralis is mainly composed of the red fibre (78.2%).…”

Section: Discussionmentioning

confidence: 99%

“…repetitive stimulation of M. digastricus evoked tetanic tension development, although the stimulus frequency necessary to evoke tetanic contraction was higher in M. digastricus than in M. semitendinosus. When repetitive stimulation, evoking incomplete tetanus, was applied to the tissue, the amplitude of the tetanus was gradually lowered in M. digastricus and gradually increased in M. semitendinosus (Y. Yamamoto, unpublished observations referred to by Ohbu, 1972).…”

mentioning

confidence: 99%

“…Recently OHBU (1972) studied the membrane properties and excitationcontraction coupling of M. digastricus of the guinea pig and concluded that two types of muscle fibre were distinguishable from their passive electrical properties. However, these two types could not be classified into fast-and slow-twitch muscles from the fine structures, active electrical properties and mechanical responses.…”

mentioning

confidence: 99%

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Comparison of Histochemical and Physiological Characteristics of M. Digastricus and M. Semitendinosus of the Guinea Pig

Yamämoto

1973

Jpn.J.Physiol

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Summary Histochemical and physiological properties of M. digastricus and M. semitendinosus of the guinea pig were investigated and compared since both muscle tissues have a red colour. 1) Action potential (overshoot potential; 14.9 mV) and membrane potential (-82.4 mV) recorded for M. digastricus were nearly the same as those obtained for M. semitendinosus (11.0 mV and -81.5 mV, respectively). 2) Both muscles generated twitch tension upon electrical stimulation; duration of tension was, however, much shorter in M. digastricus (42 msec of the mechanical response measured by the quick-release method also elucidated similar properties. 3) In the presence of various foreign anions (Br-, NO3-and SCN-), mechanical response of M. digastricus and M. semitendinosus were potentiated without any change of the membrane potential. 4) The critical membrane potentials for triggering K-induced contracture were -42 mV (70. 3mM K) in M. digastricus and -64 mV (26.7 mM K) in M. semitendinosus. These critical membrane potentials were lowered in Br--and NO3--Krebs solutions. 5) In both muscles the maximum slopes of the membrane depolarization produced by tenfold changes of [K]0 in various anions (NO3-and Br-) were the same as those observed in the presence of Cl-, but the slope became steeper in the presence of SCN-. 6) In M. digastricus, caffeine (1-5 mM) potentiated twitch tension. In a 20 mM concentration of caffeine, contracture was produced. A 0.5 mM concentration of thymol potentiated the mechanical response, produced contracture and depolarized the membrane. In M. semitendinosus, caffeine showed more marked effects than those observed in M. digastricus.

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Section: Discussionmentioning

confidence: 73%

“…Effects of caffeine and thymol on the twitch tension development were preliminary reported by OHBU (1972). In the present experiments, more detailed investigations were carried out.…”

Section: Effects Of Caffeine and Thymol On The Mechanical Responsementioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Comparison of Histochemical and Physiological Characteristics of M. Digastricus and M. Semitendinosus of the Guinea Pig

Yamämoto

1973

Jpn.J.Physiol

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Studies on the fibre types of the guinea pig masticatory muscles

Masuda

Takahashi

Kuriyama

1974

Comparative Biochemistry and Physiology Part A: Physiology

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Potassium and chloride conductances in normal and denervated rat muscles

Lorković¹,

Tomanek²

1977

American Journal of Physiology-Cell Physiology

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The membrane conductance of rat muscles (mainly gastrocnemius (G) and soleus (S)) was measured by using the two-microelectrode technique and the current point clamp method. Thin muscle bundles were washed in Tris chloride and Tris propionate solutions containing 6 mM K. The current strength was always 5 x 10-9 A and the steady-state membrane voltage was about 5 mV. The average chloride conductance (gcl, in mumho/cm2) of normal muscles was 845 (G) and 1,025 (S). The potassium conductance (gk) was 99 (G) and 161 (s). In separate measurements, the contribution of the chloride conductance (gcl) to the total membrane conductance (gm) was 89% for normal G and S. Two weeks after denervation the average gcl decreased to 440 in G and 191 in S; gk increased to 205 in G and to 364 in S in the presence of 0.1 mM Ca in solution; the increase in gk was less when measurements were made in the presence of 3mM Ca and 1 mM Mg. The contribution of gcl to gm was 60% in G and 30% in S in 14-day denervated muscles. The coefficient of variation for gcl/gk in 30 normal and 46 denervated muscles was 0.91 and 0.46, respectively. The curve representing the voltage-current relationship of muscles washed in Cl-free solutions containing 60 mM K was S-shaped in normal and in denervated muscles, but the changes in slope were less marked in the latter.

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The Membrane Properties, and Excitationcontraction Coupling of M. Digastricus of the Guinea Pig

Abstract: SummaryElectrical and mechanical properties of M. digastricus of the guinea pig were investigated by the microelectrode and strain gauge methods.

Cited by 4 publications

References 22 publications

Comparison of Histochemical and Physiological Characteristics of M. Digastricus and M. Semitendinosus of the Guinea Pig

Comparison of Histochemical and Physiological Characteristics of M. Digastricus and M. Semitendinosus of the Guinea Pig

Studies on the fibre types of the guinea pig masticatory muscles

Potassium and chloride conductances in normal and denervated rat muscles

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