The Metabolic Effects of Progesterone in Man*†

Landau, Richard L.; Bergenstal, Delbert M.; Lugibihl, Kathleen; Kascht, Marye.

doi:10.1210/jcem-15-10-1194

Cited by 200 publications

(64 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the case of progesterone, however, it is evident that this hormone enhanced the involution of pre-existing granulation tissue by means of an increased de gradation of noncollagen protein without affecting the synthetic ability for noncollagen protein. Such a concept is agreeable with the finding that significant nitrogen loss occurs during treatment with progesterone of normal human subjects (14,15).…”

Section: Resultssupporting

confidence: 84%

Anti-Inflammatory Action of Progesteroneon Carrageenin-Induced Inflammation in Rats

Nakagawa

Min

Nanjo

et al. 1979

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

Abstract-Effect of progesterone (1 mg/kg) on the inflammation in rats induced by carrageenin was studied. Progesterone inhibited the vascular permeability and the formation of granulation tissue in the early phase of the inflammation.In the chronic proliferative phase, progesterone suppressed the vascular permeability and there was an active resorption of the granulation tissue. Increased degradation of noncollagen protein was observed in the treated group without apparent influence on the metabolism of collagen or on the synthesis of noncollagen protein. It is important, therefore, to determine how progesterone in fluences the entire course of acute and chronic phases of the inflammatory processes. The present experiment was undertaken in an attempt to determine the influence of progesterone on carrageenin-induced inflammation.We used the carrageenin-air-pouch method which was developed as an experimental model covering not only acute exudative but also chronic proliferative stages of the inflammatory processes (4). MATERIALS AND METHODSAn inflammation in the form of carrageenin-air-pouch was induced on the back of male Donryu rats according to the procedure of Tsurufuji et al. (4). In rats weighing 130-160 g, 7 ml of air was injected s.c. on the back to make an air pouch. After 24 hr, 4.0 ml of 2 (w/v) % solution of Seakem 202 carrageenin (Marine Colloid Inc., Springfield, N.J., U.S.A.) was injected into the air pouch already formed. Radioiodinated human serum albumin (131I-HSA, Dainabot Co., Tokyo, 5 ttCi /mg) was used after purification by gel filtration as an indicator for the measurement of vascular permeability (5). 131I-HSA (4 ,nCi/kg in I ml of 0.9 % NaCI) was given i.v. 4 hr after the last injection of progesterone, and 30 min later the rats were sacrificed by cutting carotid artery. The entire exudate and the pouch of the granulation tissue were harvested. The exudate was centrifuged at 2,000 x g for 10 min at 4'C. The radioactivity of the supernatant (1 ml) was measured in a well type scintillation spectrometer as described in a previous paper (5). Total radioactivity in the entire pouch fluid of each rat was calculated and expressed in terms of the percentage of the radioactivity injected and used as an index of the vascular permeability of the inflamed tissue.Collagen of the granulation tissue was extracted as gelatin by autoclaving the tissue with distilled water. The extraction was done twice and the resulting collagen-free residue was referred to as the noncollagen protein fraction. The amounts and the radioactivities of collagen and noncollagen protein fractions were measured according to the procedure described previously (6). Briefly, collagen extracted as gelatin was hydrolyzed with 6 N HCI at 105'C for 16 hr. The amount of hydroxyproline in the hydrolysate was measured by the method of Kivirikko et al. (7). The radioactivity of hydroxyproline in the hydrolysate was measured after converting hydroxyproline into pyrrole which was purified by passing through a silicic acid column. The purified...

show abstract

Section: Resultssupporting

confidence: 84%

Anti-Inflammatory Action of Progesteroneon Carrageenin-Induced Inflammation in Rats

Nakagawa

Min

Nanjo

et al. 1979

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

show abstract

“…The natriuretic action of progesterone was reported in a series of articles by Landau and his co-workers (54)(55)(56). This action was demon-strated to be due to an inhibition of aldosterone by progesterone, presumably at the renal tubular level.…”

Section: Discussionmentioning

confidence: 96%

Secretion Rate of Aldosterone in Normal Pregnancy*

Watanabe¹,

Meeker²,

Gray³

et al. 1963

J. Clin. Invest.

128

View full text Add to dashboard Cite

“…Humoral agents, such as progesterone, angiotensin, and estrogen may affect renal salt handling (39)(40)(41)(42). Physical factors also must be considered.…”

Section: Discussionmentioning

confidence: 99%

Effect of hypotonic expansion on sodium, water, and urea excretion in late pregnancy: the influence of posture on these results

Lindheimer¹,

Weston²

1969

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Mineralocorticoid-treated, normotensive third trimester subjects positioned in lateral recumbency were studied before and during the infusion of 300 mEq of hypotonic saline. Urinary sodium excretion increased in all subjects from a mean value of 199 to 416 IAEq/min.In 12 maximally hydrated subjects free water clearance (CH2o) and urine flow (V) increased from means of 7.54 and 9.50 to 11.6 and 14.5 ml/100 ml of glomerular filtrate (GFR) Also the ratio of urea to inulin clearance (Curen/Cinuiln) increased from 0.59 to 0.64. The changes in the renal handling of water and urea suggest that fractional sodium reabsorption decreased at proximal nephron sites.The subjects then assumed a supine position, and the results were compared to those obtained during the lateral recumbent control periods. Filtered sodium decreased in 11 experiments, but in five studies it remained up to 2.6 mEq/min above control values. There was only one instance in which a significant increase in sodium excretion occurred. It was concluded that supine recumbency blunts natriuresis despite volume expansion or an increase in the filtered load of sodium.Finally, in the 12 hydrated subjects supine recumbency reduced CH2o and V from a mean of 11.6 and 14.5 to 6.2 and 8.2 ml/100 ml of GFR. In eight of these experiPreliminary reports of this work have been published in abstract form:

show abstract

The Metabolic Effects of Progesterone in Man*†

Cited by 200 publications

References 5 publications

Anti-Inflammatory Action of Progesteroneon Carrageenin-Induced Inflammation in Rats

Anti-Inflammatory Action of Progesteroneon Carrageenin-Induced Inflammation in Rats

Secretion Rate of Aldosterone in Normal Pregnancy*

Effect of hypotonic expansion on sodium, water, and urea excretion in late pregnancy: the influence of posture on these results

Contact Info

Product

Resources

About