The metabolic fate of C14-DDT in Triatoma infestans

Dinamarca, Maria Luisa; Agosín, Manuel R.; Neghme, A

doi:10.1016/0014-4894(62)90070-x

Cited by 23 publications

(5 citation statements)

References 12 publications

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“…1. Both metabolites are chromatographically similar to those described for BlateUa germanica (Ac, OSlN et al 1961 infestans (DINAMARCA et al 1962). Metabolite 1 is water-soluble and its chromatographic behaviour is shown in Fig.…”

Section: R E S U L T Ssupporting

confidence: 74%

“…The aqueous phase was centrifuged at 14,000 )< g for 10 min and aliquots of the supernatant fluid were chromatographed and counted. (DINAMARCA et al 1962). The combined chloroform extracts were concentrated to a suitable volume for paper chromatography as above.…”

Section: E N Z Y M E Determinationsmentioning

confidence: 99%

“…The dotted line corresponds to background radioactivity. Dye reference system as inDINAMARCA et al (1962).…”

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confidence: 99%

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Biochemical Studies on Insecticide Resistance in Musca Domestica

Gil

Fine

Dinamarca

et al. 1968

Entomologia Exp Applicata

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Microsomal metabolism of DDT is more active in housefly strains showing resistance to DDT. Susceptible as well as resistant strains produce two fairly polar metabolites in vitro (metabolites 2 and 3), and a water‐soluble metabolite (metabolite 1). Resistance to DDT in one of the strains studied (Fe) is apparently brought about by the production of large amounts of metabolite 1 in vivo and not by DDT‐dehydrochlorination. DDT‐dehydrochlorination can explain resistance per se in two strains (P2/sel and DS), but additional degrees of resistance are given in the P2/sel strain by microsomal enzyme activity. The two strains (Fc and P2/sel) which employ microsomal oxidative enzymes show induction of microsomal enzymes upon DDT pre‐treatment. This enzyme induction by DDT is accompanied by an increase in the rate of incorporation of C14‐labelled uracil into total RNA. It is suggested that cross‐resistance between DDT and pyrethrum (with associated resistance to naphthalene) may be related to microsomal enzyme induction. Zusammenfassung BIOCHEMISCHE UNTERSUCHUNGEN AN DER INSEKTIZID‐RESISTENZ VON MUSCA DOMESTICA Der mikrosomale DDT‐Stoffwechsel ist stärker bei Hausfliegenarten, die Resistenz gegen DDT aufweisen. Sowohl die empfindlichen wie die widerstandsfähigen Arten bringen in vitro zwei annähernd polare Metabolyte (Metabolyt 2 und 3) und einen wasserlöslichen Metabolyten hervor (Metabolyt 1). Die Resistenz gegen DDT bei einem der untersuchten Stämme (Fe) wird vermutlich durch das Zustandekommen grosser Mengen von Metabolyt 1 in vivo und nicht durch DDT‐Dehydrochlorination verursacht. Die DDT‐Dehydrochlorination an sich erklärt die Resistenz bei zwei Stämmen, P2/sel und DS, doch wird die Resistenz bei P2/sel noch durch mikrosomale Enzymtätigkeit erhöht. Die Stämme Fe und P2/sel, bei denen mikrosomale Oxydationsenzyme zur Wirkung kommen, weisen nach DDT‐Vorbehandlung eine Induktion der mikrosomalen Enzyme auf. Gleichlaufend mit dieser Induktion der Enzyme durch DDT erfolgt eine grössere Aufnahme von C14‐markiertem Uracyl in die RNS. Es wird vermutet, daß die “cross‐resistance” zwischen DDT und Pyrethrum (und gleichzeitiger Resistenz gegen Naphthalin) in Zusammenhang mit der mikrosomalen Enzyminduktion stehen könnte.

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Section: R E S U L T Ssupporting

confidence: 74%

Section: E N Z Y M E Determinationsmentioning

confidence: 99%

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Biochemical Studies on Insecticide Resistance in Musca Domestica

Gil

Fine

Dinamarca

et al. 1968

Entomologia Exp Applicata

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“…have not been conclusively described previously, although the possible formation of phenolic metabolites of p,p'-DDT (l,l,l-trichloro-2,2'-bis(4-chlorophenyl)ethane) has been indicated in several reports (e.g., Jensen et al, 1957;Dinamarca et al, 1962;Agosin et al, 1964;Morello, 1965;Sanchez, 1966;Schuntner and Schnitzerling, 1966;Oppenoorth and Houx, 1968;Welch et al, 1969;Agosin et al, 1969). have not been conclusively described previously, although the possible formation of phenolic metabolites of p,p'-DDT (l,l,l-trichloro-2,2'-bis(4-chlorophenyl)ethane) has been indicated in several reports (e.g., Jensen et al, 1957;Dinamarca et al, 1962;Agosin et al, 1964;Morello, 1965;Sanchez, 1966;Schuntner and Schnitzerling, 1966;Oppenoorth and Houx, 1968;Welch et al, 1969;Agosin et al, 1969).…”

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confidence: 97%

“…Metabolic hydroxylation of the aromatic rings of the para,para' isomers of DDT-type compounds (DDT, DDD, DDE, etc.) have not been conclusively described previously, although the possible formation of phenolic metabolites of , '-DDT (l,l,l-trichloro-2,2'-bis(4-chlorophenyl)ethane) has been indicated in severed reports (e.g., Jensen et al, 1957;Dinamarca et al, 1962;Agosin et al, 1964;Morello, 1965;Sanchez, 1966;Schuntner and Schnitzerling, 1966;Oppenoorth and Houx, 1968; Welch et al, 1969; Agosin et al, 1969).…”

mentioning

confidence: 99%

Metabolic hydroxylation of the aromatic rings of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (p,p'-DDE) by the rat

Sundström¹

1977

J. Agric. Food Chem.

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Zum enzymatischen Abbau des DDT 2. Mitt. Enzymatische Nebenreaktionen

Kujawa

Macholz

Engst

1985

Nahrung

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Besides alterations of the DDT molecule by enzymatic reactions on its skeleton, modifications of the phenylic rings were noted recently. Especially DDE which has been considered very stable up to now is subject to an enzymatic hydroxylation with forming some isomers. Furthermore, the isolation of methylsulfonyl derivatives of DDE succeeded. Cleavages of the phenylic ring by microbial influences and its metabolization are described too.

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The metabolic fate of C14-DDT in Triatoma infestans

Cited by 23 publications

References 12 publications

Biochemical Studies on Insecticide Resistance in Musca Domestica

Biochemical Studies on Insecticide Resistance in Musca Domestica

Metabolic hydroxylation of the aromatic rings of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (p,p'-DDE) by the rat

Zum enzymatischen Abbau des DDT 2. Mitt. Enzymatische Nebenreaktionen

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