The metabolism and biotransformation of AFB1: Key enzymes and pathways

Wang, Lingling; Huang, Qiang; Wu, Jun; Wu, Wanying; Jiang, Jun; Yan, Hongji; Huang, Jieying; Sun, Yu; Deng, Yiqun

doi:10.1016/j.bcp.2022.115005

Cited by 45 publications

(34 citation statements)

References 136 publications

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“…Coincidentally, the liver is not only the main target organ of AFB1 but also the main site of AFB1 metabolism [ 9 ]. Multiple metabolic enzymes, including CYP450s, epoxide-hydrolases, aldehyde-reductases, monooxygenases, amino-oxidases, alcohol dehydrogenases, and ketone-reductases, were demonstrated to participate in the metabolism of AFB1 [ 163 , 164 ]. It is well illustrated that AFB1 could be metabolized via two-stage reactions in the liver.…”

Section: Curcumin’s Protective Role In Preventing Afb1-induced Toxici...mentioning

confidence: 99%

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

et al. 2022

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One of the most significant classes of mycotoxins, aflatoxins (AFTs), can cause a variety of detrimental outcomes, including cancer, hepatitis, aberrant mutations, and reproductive issues. Among the 21 identified AFTs, aflatoxin B1 (AFB1) is the most harmful to humans and animals. The mechanisms of AFB1-induced toxicity are connected to the generation of excess reactive oxygen species (ROS), upregulation of CYP450 activities, oxidative stress, lipid peroxidation, apoptosis, mitochondrial dysfunction, autophagy, necrosis, and inflammatory response. Several signaling pathways, including p53, PI3K/Akt/mTOR, Nrf2/ARE, NF-κB, NLRP3, MAPKs, and Wnt/β-catenin have been shown to contribute to AFB1-mediated toxic effects in mammalian cells. Curcumin, a natural product with multiple therapeutic activities (e.g., anti-inflammatory, antioxidant, anticancer, and immunoregulation activities), could revise AFB1-induced harmful effects by targeting these pathways. Therefore, the potential therapeutic use of curcumin against AFB1-related side effects and the underlying molecular mechanisms are summarized. This review, in our opinion, advances significant knowledge, sparks larger discussions, and drives additional improvements in the hazardous examination of AFTs and detoxifying the application of curcumin.

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Section: Curcumin’s Protective Role In Preventing Afb1-induced Toxici...mentioning

confidence: 99%

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

et al. 2022

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“…If a human eats moldy cereal for a long time, liver chronic injuries would be caused, leading to the carcinogenesis and teratogenicity [4] [5].…”

Section: Introductionmentioning

confidence: 99%

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>

Wen¹,

Song²,

Cui³

et al. 2023

JBM

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Purpose: Aflatoxin B 1 is the most common mycotoxin in cereal crops; it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS 2 NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB 1 by utilizing MoS 2 NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS 2 NS applied for the detection of AFB 1 . The fluorescence of Apt@AFB 1 can be quickly quenched by MoS 2 NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB 1 exists, after the specificity binding between AFB 1 and aptamer, the Apt@AFB 1 loses its single stranded structure and is away from MoS 2 NS, and the fluorescence of Apt@AFB 1 cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB 1 . Result: With this new method, under the optimized conditions, the AFB 1 is analyzed in the MoS 2 NS/Apt@AFB 1 sensing platform. Within the dynamic range of 0.2 -25 ng/mL, the sensing platform expresses a good linear response to the level of AFB 1 with the R 2 = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% -107.23% and 95.15% -102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB 1 . This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety.

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“…Biological detoxification includes microbe‐mediated adsorption and metabolite degradation of aflatoxin. Previous studies have comprehensively summarized the classifications of degrading strains 18‐21 . Researchers were interested in the degradation of aflatoxins by proteases, including laccase, 22 oxidase, 23 peroxidase 24,25 and reductase 26 .…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have comprehensively summarized the classifications of degrading strains. [18][19][20][21] Researchers were interested in the degradation of aflatoxins by proteases, including laccase, 22 oxidase, 23 peroxidase 24,25 and reductase. 26 Sibaja et al degraded AFB 1 using commercial peroxidase; the degradation rate of AFB 1 reached 97% when H 2 O 2 was used as a cofactor.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase

Lou

Zhao

2022

J Sci Food Agric

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BACKGROUND Aflatoxin B1 (AFB1) poses a severe threat to human and animal health. Countries worldwide have invested considerable manpower and material resources in degrading aflatoxins. Enzyme degradation is the most efficient and environmentally friendly approach for modifying aflatoxin into less toxic molecules. Catalase is commonly used as a detoxification agent to decrease the contamination levels of aflatoxins in animal feeds. This study aimed to obtain recombinant catalase via gene engineering and determined whether a recombinant catalase could degrade AFB1. RESULTS The catalase gene (KatA) from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli, and the expression conditions of this recombinant catalase were optimized. The recombinant catalase was isolated and purified using Ni‐chelating affinity chromatography, and its ability to degrade AFB1 was evaluated. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis showed that the expressed of catalase was approximately 55.6 kDa, which was subsequently purified using Ni‐chelating affinity chromatography. The degradation rate of AFB1 by recombinant catalase in the presence of syringaldehyde was 38.79%. CONCLUSION The degradation of AFB1 by a recombinant catalase has been reported for the first time. This study provides a new paradigm for the use of recombinant catalases in degrading AFB1 in food and feed. © 2022 Society of Chemical Industry.

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The metabolism and biotransformation of AFB1: Key enzymes and pathways

Cited by 45 publications

References 136 publications

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase

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The metabolism and biotransformation of AFB1: Key enzymes and pathways

Cited by 45 publications

References 136 publications

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB&lt;sub&gt;1&lt;/sub&gt;

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB1 by recombinant catalase

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Product

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Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase