The metabolism of C2 compounds in micro-organisms. 4. Synthesis of cell materials from acetate by Aspergillus niger

The Metabolism of 2‐Carbon Compounds by Microorganisms

“…This was confirmed in studies of the isocitratase of Aspergillus niger (22) and Micrococcus denitriJicans (14) ; the enzyme from Micrococcus denitrificans was more than 10 times as sensitive to the presence of succinate ( K , = 3 X 10-3M) as the enzymes obtained from the other two organisms. Working with extracts of E. coZi, Umbarger (54) found that isocitratase was powerfully inhibited also by oxaloacetate, whereas pyruvate, malate, fumarate, or aspartate had no effect.…”

Memoirs of a Biochemical Hod Carrier

“…I was warmly welcomed also by his colleagues, particularly Andrew A. Benson, Al Bassham, Clinton Fuller, and J. Rodney Quayle, who taught me the techniques used for the isolation and analysis of labeled products formed after incubating microorganisms with 14 C-labeled substrates for very short time periods. These techniques enabled me, in later years, to undertake studies on the metabolism of C 2 compounds in bacteria (8), fungi (9), and algae (10) that also propelled me into paths I still follow.…”

Enrichment, isolation and characterization of fungi tolerant to 1-ethyl-3-methylimidazolium acetate

“…We have demonstrated through three different cultivation methods that common Aspergilli isolated from soil and compost possess high tolerances to [C2mim][OAc] and can use this ionic liquid as their sole carbon source for growth. The absence of growth of these strains in the presence of [C4mim] [Cl] suggests that the fungi utilize the acetate as the source of carbon; the ability of Aspergillus isolates to grow with acetate as the sole carbon source has been extensively documented (Collins and Kornberg 1960;Armitt et al 1970;Meijer et al 2009). These results are also consistent with previous observations that the imidazolium ring is resistant to biodegradation and may persist in the environment (Docherty and Kulpa 2005).…”