The metabolism of d- and l-3-hydroxybutyrate in developing rat brain

Stereoselective effects of 3-hydroxybutyrate on glucose utilization of rat cardiomyocytes

“…This result may suggest that l-3HB is metabolized in a pathway different from that of d-3HB, i.e., no generation of NADH or acetyl CoA during l-3HB metabolism. It had also shown that the oxidation rate of l-3HB was lower than that of d-3HB (Swiatek et al, 1981(Swiatek et al, , 1984. Therefore, unlike other ketone bodies, l-3HB may not be utilized for energy supplements.…”

“…It was surprising to find that DL-3HB was the more preferred precursor for biosynthesis of lipids than acetoacetate when DL-3HB and acetoacetate were injected in equal amounts (Edmond, 1974). Subsequent studies showed l-3HB as well as d-3HB can be incorporated into hepatic lipids, brain proteins, and amino acids during the developmental period of neonatal rats (Swiatek et al, 1981(Swiatek et al, , 1984. In addition, l-3HB was shown to be a more-favorable substrate than other ketone bodies for sterol and fatty acid synthesis in the brain, spinal cord, and kidney (Webber and Edmond, 1977).…”

Highly efficient ketone body treatment in multiple acyl-CoA dehydrogenase deficiency–related leukodystrophy

“…Little is known about the uptake and metabolization of free L-3-hydroxybutyrate by peripheral organs and its physiological importance in humans. In rats, it has been shown, that the L-enantiomer is produced by the liver (17) and can be utilized by the developing brain (18) and that there is a stereoselective effect of the enantiomers in cardiomyocytes (19). L-3-hydroxybutyrate may be thus converted to physiological ketone bodies, diverted to biosynthesis of lipids, sterols and other complex molecules or fully metabolized to CO2 by the Krebs cycle (20,21).…”

Establishment of a two-dimensional chiral HPLC system for the simultaneous detection of lactate and 3-hydroxybutyrate enantiomers in human clinical samples

“…A previous study using enzymatic method showed that when the concentration of L-3HB is less than 5% in D/L-3HB, L-3HB most likely could not be detected [30]. In our previous study using the conventional heart-cutting column-switching HPLC method, the D-3HB content in rat serum was found to be around 4% [26].…”