The metabolism of glycerol in Mycobacterium butyricum

Enzyme Systems in the Mycobacteria Xi

“…We present here evidence that the glycolytic sequence operates in M. tuberculosis and that the component enzymes of this sequence are essentially identical with those previously isolated from animal tissues. This evidence, with that of Edson et al (1959), indicates that glycolysis is probably the first metabolic sequence involved in the oxidative assimilation of glycerol.…”

“…94 presented by Hunter (1953) for the presence in these mycobacteria of a-glycerol phosphate dehydrogenase, enolase and, possibly, pyruvate kinase. The oxidation of glycerol through pyruvate and the tricarboxylic acid cycle to CO2 and water by washed cells of M. butyricum has been studied by Edson et al (1959). These authors demonstrated through the use of labeled glycerol, bicarbonate, and acetate that the glycolytic and tricarboxylic acid cycle reactions account for the majority of the glycerol oxidized by this organism.…”

The Formation of Keto and Amino Acids by Mycobacterium butyricum Growing in a Chemically Defined Medium

“…The nitrogen fraction designated 'other forms' includes protein (cf. Edson et al 1959) and peptide nitrogen and unidentified nitrogen compounds.…”

“…This is possible because M . butyricum contains isocitritase (Morrison & Wright, 1958) and WO, is fixed in the requisite carboxyl group of isocitrate (Edson et al 1959). …”

“…The bacteria were defatted (Edson et al 1959) and samples (20 mg.) of the residue hydrolysed in sealed tubes with 5 ml. of ~N -H C~ at 100" for 8 hr.…”