The metabolism of leukotriene B4 by peripheral blood polymorphonuclear leukocytes in psoriasis

Maurice, P; Camp, R.D.R.; Allen, B.R.

doi:10.1016/0090-6980(87)90109-2

Cited by 8 publications

(1 citation statement)

References 13 publications

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“…After precipitation of proteins with acetonitrile, the metabolites of LTB 4 were measured in the incubate of A23187-stimulated PMNs by HPLC assay based on those described by Shak and Goldstein [14] and Maurice et al [15]. We used ChromSpher C18 column with methanol:water:acetic acid:ammonia 55:45:0.1:0.1 (v:v:v:v:v) (pH adjusted to 4.0) as a mobile phase.…”

Section: -Oh-ltb 4 and 20-cooh-ltbmentioning

confidence: 99%

Eicosanoid production in rheumatoid synovitis

Moilanen

Alanko

Nissilä³

et al. 1989

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Leukotriene B4 (LTB4) synthesis in rheumatoid synovitis was studied using peripheral and synovial fluid polymorphonuclear leukocytes (PMNs) and rheumatic synovial lining cells. No differences were found in LTB4 synthesis between peripheral PMNs from healthy volunteers and rheumatoid arthritis patients. When peripheral and synovial PMNs from the same RA patient were compared, arachidonic acid-induced LTB4 synthesis in synovial fluid PMNs was increased 1.7-7.2 fold, whereas the response to Ca ionophore A23187 stimulation was similar. This suggests 5-lipoxygenase stimulating factor(s) in inflamed joints. Rheumatic synovial lining cells in a primary cell culture produced small amounts of LTB4, the concentrations being less than 0.1 per cent of those of prostaglandin E2 (PGE2). PGE2 synthesis in synovial cells was increased when arachidonic acid or interleukin-1 was added to the culture, whereas LTB4 production remained unaltered. The present results suggest that in inflamed joints LTB4 originates mainly from PMNs whereas synovial lining cells are the source for PGE2.

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Section: -Oh-ltb 4 and 20-cooh-ltbmentioning

confidence: 99%

Eicosanoid production in rheumatoid synovitis

Moilanen

Alanko

Nissilä³

et al. 1989

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Metabolism of exogenous arachidonic acid by polymorphonuclear leukocytes from psoriatic patients

Vilá

Puig

et al. 1991

Arch Dermatol Res

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We evaluated the metabolism of exogenous arachidonic acid in polymorphonuclear leukocytes from psoriatic patients in comparison with those from normal subjects. The leukocytes were incubated with 10 microM labelled arachidonic acid and 5 microM A23187 for varying periods of time. We evaluated all the compounds derived from the 5-lipoxygenase activity, including the products of non-enzymatic transformation of leukotriene A4 and w-oxidized leukotriene B4. In the experimental conditions used, there was no significant difference in the formation of any compound at any time of incubation. These results indicate that there is no intrinsic alteration either in 5-lipoxygenase activity or in w-oxidation ability of circulating polymorphonuclear leukocytes in psoriatic patients.

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Inflammatory mediators and chemoattractants

Schröder

1995

Clinics in Dermatology

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The metabolism of leukotriene B4 by peripheral blood polymorphonuclear leukocytes in psoriasis

Cited by 8 publications

References 13 publications

Eicosanoid production in rheumatoid synovitis

Eicosanoid production in rheumatoid synovitis

Metabolism of exogenous arachidonic acid by polymorphonuclear leukocytes from psoriatic patients

Inflammatory mediators and chemoattractants

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