The metabolism of membrane lipid participates in the occurrence of chilling injury in cold-stored banana fruit

Li, Qian; Lin, Han; Lin, He-tong; Lin, Meng-shi; Wang, Hui; Wei, Wei; Chen, Jian-ye; Lu, Wang-jin; Shao, Xing-feng; Fan, Zhong-qi

doi:10.1016/j.foodres.2023.113415

Cited by 24 publications

(7 citation statements)

References 49 publications

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“…One group was stored at 7 °C (cold treatment); the other group was stored at 22 °C (control). Banana peel tissues were sampled at 0, 1, 2, 3, 4, and 5 d; frozen in liquid nitrogen; and preserved at −80 °C for further use [ 4 ].…”

Section: Methodsmentioning

confidence: 99%

“…Banana ( Musa acuminata ) is one of the most widely grown fruits in tropical and subtropical regions due to its sweet flavor and high nutritional value [ 1 , 2 ]. However, banana is a typical cold-sensitive fruit that is susceptible to chilling injury (CI) symptoms, including pitting, water soaking, and even abnormal fruit ripening, leading to a quality deterioration and commodity loss [ 3 , 4 ]. Therefore, it is important to investigate the underlying molecular mechanism of CI development in postharvest banana fruit.…”

Section: Introductionmentioning

confidence: 99%

“…LOX promotes membrane lipids’ peroxidation via catalyzing the conversion of unsaturated fatty acids (USFAs) to saturated fatty acids (SFAs) [ 11 ]. Previous reports have shown that the enzymatic activity and the transcription of PLA, PLD, LOX, and lipase in banana fruit were induced by a cold environment during storage [ 4 ]. Moreover, the enzyme activity and gene expression of PLD and LOX increased in cold-stored green bell peppers [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we demonstrated that CI development in banana fruit was caused by membrane integrity damage and was associated with the enzymatic and genetic manipulation of membrane lipid metabolism. These activities facilitated the degradation of membrane phospholipids and USFAs in banana fruit during the cold storage period [ 4 ]. Nevertheless, the transcriptional regulatory mechanisms underpinning WRKY-TF-modulated membrane lipid metabolism in cold-stored banana fruit remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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The Role of MaWRKY70 in Regulating Lipoxygenase Gene Transcription during Chilling Injury Development in Banana Fruit

Lin,

Bai,

Wei

et al. 2024

Foods

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Banana is a typical cold-sensitive fruit; it is prone to chilling injury (CI), resulting in a quality deterioration and commodity reduction. However, the molecular mechanism underlying CI development is unclear. In this study, cold storage (7 °C for 5 days) was used to induce CI symptoms in bananas. As compared with the control storage (22 °C for 5 days), cold storage increased the CI index and cell membrane permeability. Moreover, we found that the expression levels of the WRKY transcription factor MaWRKY70 were increased consistently with the progression of CI development. A subcellular localization assay revealed that MaWRKY70 was localized in the nucleus. Transcriptional activation analyses showed that MaWRKY70 processed a transactivation ability. Further, an electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assays showed that MaWRKY70 was directly bound to the W-box motifs in the promoters of four lipoxygenase (LOX) genes associated with membrane lipid degradation and activated their transcription. Collectively, these findings demonstrate that MaWRKY70 activates the transcription of MaLOXs, thereby acting as a possible positive modulator of postharvest CI development in banana fruit.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Role of MaWRKY70 in Regulating Lipoxygenase Gene Transcription during Chilling Injury Development in Banana Fruit

Lin,

Bai,

Wei

et al. 2024

Foods

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“…Wang et al, 2016) and increases the accumulation of malondialdehyde (MDA), the marker of lipid peroxidation (Q. Li et al, 2023). This leads to quality deterioration and cell death (Liang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Melatonin: A biomolecule for mitigating postharvest chilling injury in fruits and vegetables

Sati,

Kataria,

Chinchkar

et al. 2023

Crop Science

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The best strategy to extend the shelf life of fresh horticultural produce is to keep it at a low temperature. However, storing fruits and vegetables at a temperature lower than their recommended storage temperature and above the freezing point for a prolonged time leads to the development of chilling injury (CI). Melatonin (MT) being a versatile biological signal has been reported to possess antioxidant properties that aid in mediating stress response and extending the postharvest storage life of horticultural crops. Among CI‐induced mechanisms, MT substantially reduces oxidative stress by elevating the reactive oxygen species scavenging and triggering the phenol accumulation in association with 2, 2‐diphenyl‐1 picryl hydrazyl scavenging activity. Besides, MT increases intracellular energy by maintaining a proper ratio of unsaturated to saturated fatty acid and improving cellular fluidity. Additionally, MT regulates arginine metabolism by maintaining the γ‐aminobutyric acid synthesis. Furthermore, MT encodes the mitogen‐activated protein kinases, and calcium‐dependent protein kinases cascade and acclimate to cold stress by activating C‐repeat binding factors and helps in maintaining postharvest quality. This review reports the recent progress of MT‐induced physiological, biochemical, and molecular mechanisms for enhancing chilling tolerance, which can elevate the marketability and earnings from postharvest horticultural crops.

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Melatonin alleviated chilling injury of cold-stored passion fruit by modulating cell membrane structure via acting on antioxidant ability and membrane lipid metabolism

Lin,

Chen,

Chen

et al. 2025

Current Research in Food Science

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The metabolism of membrane lipid participates in the occurrence of chilling injury in cold-stored banana fruit

Cited by 24 publications

References 49 publications

The Role of MaWRKY70 in Regulating Lipoxygenase Gene Transcription during Chilling Injury Development in Banana Fruit

The Role of MaWRKY70 in Regulating Lipoxygenase Gene Transcription during Chilling Injury Development in Banana Fruit

Melatonin: A biomolecule for mitigating postharvest chilling injury in fruits and vegetables

Melatonin alleviated chilling injury of cold-stored passion fruit by modulating cell membrane structure via acting on antioxidant ability and membrane lipid metabolism

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