The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)

Zhou, Chengxu; Luo, Jie; Ye, Yangfang; Yan, Xiaojun; Liu, Baoning; Wen, Xin

doi:10.1007/s00343-016-5042-0

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…Sharp resonance signals due to amino acids (valine, isoleucine, leucine, threonine, alanine, proline, methionine, glutamate, glutamine, lysine, and aspartate) and organic acids (acetate, lactate, and succinate) appear in this region and were assigned in accordance with the previous literature and matching chemical shifts retrieved from databases cited previously. Many of these metabolites were also found in the microalgae Pleurochrysis carterae (under the same phylum Haptophyta) by Zhou et al and in the diatom Chaetoceros calcitrans as reported by Azizan et al A series of broad peaks also appear in this region and are attributable to the fatty acids, whose assignment was previously discussed in the analysis of chloroform/methanol (80:20 v/v) extracts. 2D NMR experiments helped us in the analysis of more difficult assignments owing to overlapping of other signals, such as the broad signals of fatty acids.…”

Section: Resultssupporting

confidence: 59%

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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This study assessed the feasibility of an NMR metabolomics approach coupled to multivariate data analysis to monitor the naturally present or stresses-elicited metabolites from a long-term (>170 days) culture of the dinoflagellate marine microalgae Amphidinium carterae grown in a fiberglass paddlewheel-driven raceway photobioreactor. Metabolic contents, in particular, in two members of the amphidinol family, amphidinol A and its 7-sulfate derivative amphidinol B (referred as APDs), and other compounds of interest (fatty acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration levels of the f/2 medium nutrients and daily mean irradiance. Operating with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD production, which was also detected by an increase in hemolytic activity of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral activity measured in the methanolic extracts of the biomass. Increased daily irradiance was accompanied by a general decrease in pigments and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated fatty acids), and DHA (docosahexaenoic acid), while increased nutrient availability lead to an increase in sugar, amino acid, and PUFA ω-3 contents and pigments and a decrease in SFAs and MUFAs. NMR-based metabolomics is shown to be a fast and suitable method to accompany the production of APD and bioactive compounds without the need of tedious isolation methods and bioassays. The two APD compounds were chemically identified by spectroscopic NMR and spectrometric ESI-IT MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF MS (ESI time-of-flight mass spectrometry) methods.

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Section: Resultssupporting

confidence: 59%

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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Acetylated Nucleoside Derivatives from a Shallow-Water Marine Bivalve Codakia orbicularis

et al. 2019

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Mechanisms driving genome reduction of a novel Roseobacter lineage

et al. 2021

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Summary Members of the marine Roseobacter group are key players in the global carbon and sulfur cycles. While over 300 species have been described, only 2% possess reduced genomes (mostly 3–3.5 Mbp) compared to an average roseobacter (>4 Mbp). These taxonomic minorities are phylogenetically diverse but form a Pelagic Roseobacter Cluster (PRC) at the genome content level. Here, we cultivated eight isolates constituting a novel Roseobacter lineage which we named ‘CHUG’. Metagenomic and metatranscriptomic read recruitment analyses showed that CHUG members are globally distributed and active in marine pelagic environments. CHUG members possess some of the smallest genomes (~2.6 Mb) among all known roseobacters, but they do not exhibit canonical features of typical bacterioplankton lineages theorized to have undergone genome streamlining processes, like higher coding density, fewer paralogues and rarer pseudogenes. While CHUG members form a genome content cluster with traditional PRC members, they show important differences. Unlike other PRC members, neither the relative abundances of CHUG members nor their relative gene expression levels are correlated with chlorophyll a concentration across the global samples. CHUG members cannot utilize most phytoplankton-derived metabolites or synthesize vitamin B12, a key metabolite mediating the roseobacter-phytoplankton interactions. This combination of features is evidence for the hypothesis that CHUG members may have evolved a free-living lifestyle decoupled from phytoplankton. This ecological transition was accompanied by the loss of signature genes involved in roseobacter-phytoplankton symbiosis, suggesting that relaxation of purifying selection owing to lifestyle shift is likely an important driver of genome reduction in CHUG.

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The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)

Cited by 5 publications

References 39 publications

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Acetylated Nucleoside Derivatives from a Shallow-Water Marine Bivalve Codakia orbicularis

Mechanisms driving genome reduction of a novel Roseobacter lineage

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