The metabolomics of oxidative stress

Noctor, Graham; Lelarge-Trouverie, Caroline; Mhamdi, Amna

doi:10.1016/j.phytochem.2014.09.002

Cited by 219 publications

(178 citation statements)

References 231 publications

(309 reference statements)

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“…The up-regulation of similar transferase genes together with those of cytochrome P450s has been shown to be typical for a catalasedeficient Arabidopsis mutant that suffers from oxidative stress (Queval et al, 2012;Noctor et al, 2015). Glutathione S-transferases, for example, catalyze the conjugation of oxidized lipid compounds to glutathione (Farmer and Mueller, 2013;Noctor et al, 2015). In conditions of H 2 O 2 scavenging, several genes encoding putative ascorbate oxidases were induced (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…9B) and UDP-Glc/GlcA transferases in lignin-forming conditions suggests that the synthesis of glutathione and sugar conjugates was increased during lignin formation, further supporting that cells under lignin-forming conditions were subjected to oxidative stress. The up-regulation of similar transferase genes together with those of cytochrome P450s has been shown to be typical for a catalasedeficient Arabidopsis mutant that suffers from oxidative stress (Queval et al, 2012;Noctor et al, 2015). Glutathione S-transferases, for example, catalyze the conjugation of oxidized lipid compounds to glutathione (Farmer and Mueller, 2013;Noctor et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

et al. 2017

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Apoplastic events such as monolignol oxidation and lignin polymerization are difficult to study in intact trees. To investigate the role of apoplastic hydrogen peroxide (H 2 O 2 ) in gymnosperm phenolic metabolism, an extracellular lignin-forming cell culture of Norway spruce (Picea abies) was used as a research model. Scavenging of apoplastic H 2 O 2 by potassium iodide repressed lignin formation, in line with peroxidases activating monolignols for lignin polymerization. Time-course analyses coupled to candidate substrate-product pair network propagation revealed differential accumulation of low-molecular-weight phenolics, including (glycosylated) oligolignols, (glycosylated) flavonoids, and proanthocyanidins, in lignin-forming and H 2 O 2 -scavenging cultures and supported that monolignols are oxidatively coupled not only in the cell wall but also in the cytoplasm, where they are coupled to other monolignols and proanthocyanidins. Dilignol glycoconjugates with reduced structures were found in the culture medium, suggesting that cells are able to transport glycosylated dilignols to the apoplast. Transcriptomic analyses revealed that scavenging of apoplastic H 2 O 2 resulted in remodulation of the transcriptome, with reduced carbon flux into the shikimate pathway propagating down to monolignol biosynthesis. Aggregated coexpression network analysis identified candidate enzymes and transcription factors for monolignol oxidation and apoplastic H 2 O 2 production in addition to potential H 2 O 2 receptors. The results presented indicate that the redox state of the apoplast has a profound influence on cellular metabolism.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

et al. 2017

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“…Accumulation of GSSG is the clearest and most readily quantifiable biochemical marker of altered redox state caused by a decreased capacity for the removal of intracellular H 2 O 2 (Willekens et al, 1997;Queval et al, 2009Queval et al, , 2011. By comparison, reproducible increases in H 2 O 2 itself are much more difficult to detect in cat2 (Mhamdi et al, 2010a;Noctor et al, 2015). This suggests that, when catalase capacity is decreased, alternative H 2 O 2 -reducing reactions are readily elicited, dampening increases in H 2 O 2 and instead leading to the oxidation of key redox buffers such as glutathione.…”

Section: Dhars Cooperate In Glutathione Oxidation Triggered By Oxidatmentioning

confidence: 99%

Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

Rahantaniaina

Li²,

Châtel-Innocenti³

et al. 2017

Plant Physiol.

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The complexity of plant antioxidative systems gives rise to many unresolved questions. One relates to the functional importance of dehydroascorbate reductases (DHARs) in interactions between ascorbate and glutathione. To investigate this issue, we produced a complete set of loss-of-function mutants for the three annotated Arabidopsis (Arabidopsis thaliana) DHARs. The combined loss of DHAR1 and DHAR3 expression decreased extractable activity to very low levels but had little effect on phenotype or ascorbate and glutathione pools in standard conditions. An analysis of the subcellular localization of the DHARs in Arabidopsis lines stably transformed with GFP fusion proteins revealed that DHAR1 and DHAR2 are cytosolic while DHAR3 is chloroplastic, with no evidence for peroxisomal or mitochondrial localizations. When the mutations were introduced into an oxidative stress genetic background (cat2), the dhar1 dhar2 combination decreased glutathione oxidation and inhibited cat2-triggered induction of the salicylic acid pathway. These effects were reversed in cat2 dhar1 dhar2 dhar3 complemented with any of the three DHARs. The data suggest that (1) DHAR can be decreased to negligible levels without marked effects on ascorbate pools, (2) the cytosolic isoforms are particularly important in coupling intracellular hydrogen peroxide metabolism to glutathione oxidation, and (3) DHAR-dependent glutathione oxidation influences redox-driven salicylic acid accumulation.

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“…Probably, microalgae attempts to compensate this effect by increasing its fucoxanthin content. Different types of mechanisms acting alone or in interaction may cause the change in fucoxanthin level by modifiying enzyme activities through transcriptional or post-transcriptional mechanisms that activate signalling or defence mechanisms (Noctor et al,, 2015).…”

Section: Effect Of Oxidative Stress On Fucoxanthin Production During mentioning

confidence: 99%

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Erdoğan¹,

Demirel²,

Dalay³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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Production of fucoxanthin by diatoms has become an alternative research area due to their low cost, convenience and diversity. The fucoxanthin content of Cylindrotheca closterium and its enhancement by altering the cultivation conditions via oxidative stress were investigated in this study. For this purpose, the extraction parameters were optimized and the highest fucoxanthin concentration (6.58 mg g -1 ) was achieved within 15.0 minutes at 40 o C. Then, this yield reached to 10.15 mg g -1 in the presence of NaOCl and Fe 2+ . It is worth noting that, this is the first time that the effect of oxidative stress on fucoxanthin production in diatom has been studied according to our knowledge. Therefore, the results of this study and the discussion about the mechanisms can be a reference for the enrichment of fucoxanthin from other diatoms.Keywords: Fucoxanthin; Cylindrotheca closterium, oxidative stress; diatom; extraction. Cylindrotheca closterium'un Fukoksantin İçeriği ve Oksidatif Stres ile Arttırılması ÖzetFukoksantinin diatomlar tarafından üretimi düşük maliyet, kolaylık ve çeşitlilik nedeniyle alternatif bir araştırma alanı haline gelmiştir. Bu çalışmada, Cylindrotheca closterium'da bulunan fukoksantin miktarı ve kültür koşullarına uygulanan oksidatif stresin, bu içeriği nasıl arttırdığı araştırılmıştır. Bu amaçla,ekstraksiyon parametreleri optimize edilmiş ve en yüksek fukoksantin derişimi ( 6,58 mg g -1 ) 40 o C''de ve 15,0 dakika içinde elde edilmiştir. Daha sonra, fukoksantin miktarı, NaOCl ve Fe 2+ varlığında 10,15 mg g -1 a kadar ulaşmıştır. Bu çalışma ile ilk kez bir diatomun kültür koşullarına uygulanan oksitafis stres ile fukoksantin miktarındaki değişim gözlenmiştir. Bu nedenle; söz konusu çalışma, sonuçları ve mekanizmalar hakkında yapılan değerlendirmeler ile fukoksantinin diğer diatomlarda da zenginleştirilmesi açısından bir referans olabilir.

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The metabolomics of oxidative stress

Cited by 219 publications

References 231 publications

A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

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The metabolomics of oxidative stress

Cited by 219 publications

References 231 publications

A Key Role for Apoplastic H2O2 in Norway Spruce Phenolic Metabolism

A Key Role for Apoplastic H2O2 in Norway Spruce Phenolic Metabolism

Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

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A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism