The micro-CT evaluation of enamel-cement thickness, abrasion, and mineral density in teeth in the postmortem interval (PMI): new parameters for the determination of PMI

Akbulut, Nıhat; Çeti̇n, Selçuk; Bilecenoğlu, Burak; Altan, Ahmet; Akbulut, Sibel; Ocak, Mert; Orhan, Kaan

doi:10.1007/s00414-019-02104-2

Cited by 25 publications

(20 citation statements)

References 23 publications

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“…In order to analyze the ROI of the whole specimen, the optimal grey value range was determined in the histogram. After the segmentation of the image, the software itself performed the analysis of micro morphometric parameters in an automatic way [43]. The morphometric variables relevant to our study calculated by CTAn software are listed in Table 1 [44,45].…”

Section: Micro-ct Imaging Analysismentioning

confidence: 99%

Comparison between Micro-Computed Tomography and Cone-Beam Computed Tomography in the Assessment of Bone Quality and a Long-Term Volumetric Study of the Augmented Sinus Grafted with an Albumin Impregnated Allograft

Kivovics

Szabó

Németh

et al. 2020

JCM

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The purpose of our study was to compare micromorphometric data obtained by cone-beam computed-tomography (CBCT) and microcomputed-tomography (micro-CT) of the augmented sinus and to evaluate the long-term stability of the bone gain achieved using BoneAlbumin. Sinus lifts, and after 6-months, healing bone-biopsy and implant placement were carried out. Specimens were analyzed by micro-CT. A total of 16 samples were collected from nine patients (mean age 54.7 ± 6.5 years). Pre-, postoperative, and 3-year control CBCT-data were registered to determine from where the biopsy samples were harvested. Micromorphometric variables were calculated from the micro-CT- and CBCT-data, and their correlation was determined by Spearman’s test. The volume of augmented bone was calculated at the time of implant placement and after 3 years. A positive correlation was found between bone-volume fraction, trabecular-separation, open-, and total-porosity, while a negative correlation was found between trabecular-thickness obtained from CBCT- and micro-CT-data (p < 0.05). Mean volumetric reduction of 39.28% (11.88–60.02%) was observed. Correlation of CBCT- and micro-CT-data suggested that micromorphometric analysis of CBCT reconstructions of the augmented sinuses provided reliable information on the microarchitecture of augmented bone. CBCT as a modality might be adequate in the analysis of bone quality in the augmented sinus. At the 3-year, control sinus grafts showed volumetric stability.

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Section: Micro-ct Imaging Analysismentioning

confidence: 99%

Comparison between Micro-Computed Tomography and Cone-Beam Computed Tomography in the Assessment of Bone Quality and a Long-Term Volumetric Study of the Augmented Sinus Grafted with an Albumin Impregnated Allograft

Kivovics

Szabó

Németh

et al. 2020

JCM

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“…In particular, in cases of skeletal remains, the PMI becomes very difficult to estimate because soft tissues decrease substantially or completely. Many researchers have focused on the development of novel methods, including infrared (IR)/Raman microscopic imaging techniques [1,2], inductively coupled plasma optical emission spectroscopy (ICP-OES) [3], or microcomputed tomography (µCT) [4,5]. In addition, combinations of techniques have been reported, such as the combined use of different physicochemical methods [6], luminol and microscopic techniques to screen bones before the use of radiocarbon dating [7], ultraviolet (UV)-induced fluorescence and 490 nm induced fluorescence [8], and µCT, mid-infrared microscopic imaging, and energy-dispersive X-ray mapping [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study

et al. 2020

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The postmortem interval (PMI) of victims is a key parameter in criminal investigations. However, effective methods for estimating the PMI of skeletal remains have not been established because it is determined by various factors, including environmental conditions. To identify effective parameters for estimating the PMI of skeletal remains, we investigated the change in bone focusing on the amount of DNA, element concentrations, and bone density that occurred in the bone samples of bovine femurs, each maintained under one of five simulated environmental conditions (seawater, freshwater, underground, outdoors, and indoors) for 1 year. The amount of extracted mitochondrial DNA (mtDNA; 404 bp fragment) decreased over time, and significant DNA degradation (p < 0.01), as estimated by a comparison with amplification results for a shorter fragment (128 bp), was detected between 1 month and 3 months. Eleven of 30 elements were detected in samples by inductively coupled plasma optical emission spectrometry, and Na and Ba showed significant quantitative differences in terms of environmental conditions and time (p < 0.01). This preliminary study suggests that the level of DNA degradation determined by real-time polymerase chain reaction and element concentrations determined by inductively coupled plasma optical emission may be useful indices for estimating the PMI of victims under a wide range of environmental conditions. However, this study is a limited experimental research and not applicable to forensic cases as it is. Further studies of human bone with longer observation periods are required to verify these findings and to establish effective methods for PMI estimation.

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“…We also utilized micro-CT to assess changes in the volume of enamel surfaces at different stages of the experiment. Micro-CT is a non-destructive method of investigating enamel surface changes that provides three-dimensional (3D) high-definition images and has been used by various researchers to study volumetric changes in enamel [ 38 , 39 ]. In an earlier micro-CT study, Farooq et al reported a significant increase in enamel surface volume when they were immersed in a mixture of F-BG toothpaste and AS as compared with Novamin TM and AS mixture [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Enamel Remineralization Competence of a Novel Fluoride-Incorporated Bioactive Glass Toothpaste—A Surface Micro-Hardness, Profilometric, and Micro-Computed Tomographic Analysis

et al. 2021

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This study aimed to analyze the enamel remineralization efficacy of a novel fluoridated bioactive glass (F-BG) toothpaste compared to a standard fluoride toothpaste. Seventy-two enamel blocks (N = 72) were divided into groups of twenty-four blocks according to the toothpaste exposure—group 1: brushed with distilled water, group 2: brushed with fluoride toothpaste (ColgateTM), and group 3: brushed with F-BG toothpaste (BioMinFTM). Pre-brushing, enamel blocks were demineralized using 6 wt.% citric acid (pH = 2.4). Tooth brushing was performed using a mixture of respective toothpaste and artificial saliva (AS), and each enamel block received 5000 linear strokes. The samples were assessed for surface micro-hardness (to estimate Vickers hardness number, VHN), surface roughness (Ra), and volume loss/gain using micro-computed tomography (micro-CT). The highest increase in the VHN was noticed for group 3 (117.81) followed by group 2 (61.13), and all the intragroup comparisons were statistically significant (p < 0.05). Demineralization increased the Ra values, and a decrease was observed post-remineralization for all the groups. The maximum Ra decrease was observed for group 3 (−223.2 nm) followed by group 2 (−55.6 nm), and all the intragroup comparisons were again statistically significant (p < 0.05). Micro-CT investigation revealed that the enamel volume decreased after demineralization and increased after remineralization among all groups. The F-BG toothpaste showed greater enamel surface micro-hardness (increased VHN), smoother surface (low roughness), and better volume restoration (remineralization) in comparison to the fluoride toothpaste.

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The micro-CT evaluation of enamel-cement thickness, abrasion, and mineral density in teeth in the postmortem interval (PMI): new parameters for the determination of PMI

Cited by 25 publications

References 23 publications

Comparison between Micro-Computed Tomography and Cone-Beam Computed Tomography in the Assessment of Bone Quality and a Long-Term Volumetric Study of the Augmented Sinus Grafted with an Albumin Impregnated Allograft

Comparison between Micro-Computed Tomography and Cone-Beam Computed Tomography in the Assessment of Bone Quality and a Long-Term Volumetric Study of the Augmented Sinus Grafted with an Albumin Impregnated Allograft

Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study

Enamel Remineralization Competence of a Novel Fluoride-Incorporated Bioactive Glass Toothpaste—A Surface Micro-Hardness, Profilometric, and Micro-Computed Tomographic Analysis

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