The microbial cell — functional unit for energy dependent multistep biocatalysis

Ladkau, Nadine; Schmid, Andreas; Bühler, Bruno

doi:10.1016/j.copbio.2014.06.003

Cited by 62 publications

(44 citation statements)

References 54 publications

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“…Whole-cell biocatalysts allow for the facile implementation of enzymatic cascades that span multiple reactions, with an integrated supply of the myriad cofactors that are needed for such complex biotransformations [12]. This internal supply greatly simplifies cofactor regeneration and makes the addition of expensive external cofactors unnecessary.…”

Section: Advantages Of Whole-cell Biocatalysismentioning

confidence: 99%

“…In fermentations, the products are synthesized from growth substrates via the host cells’ native metabolism and are accompanied in the fermentation broth by metabolic intermediates that make downstream processing complicated [11, 12]. In biotransformations, cell growth (the enzyme manufacturing phase) and production phase are separated.…”

Section: Introductionmentioning

confidence: 99%

“…Here, Pv is the volumetric productivity in the unit of g/L/h. Cheap raw materials, efficient bioconversion, and the ability to reuse the biocatalyst many times reduce the cost of product and make whole-cell biocatalysis very cost-competitive with fermentation [12, 15–19]. …”

Section: Introductionmentioning

confidence: 99%

“…This dominance is largely because of its well-studied genetic background, a mature and powerful genetic toolset for metabolic engineering, and relatively well-developed fermentation processes with low-cost raw materials [12, 13, 16]. Whole-cell biocatalysis has even been implemented by coupling two recombinant strains [22–24].…”

Section: Introductionmentioning

confidence: 99%

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Whole-cell biocatalysts by design

2017

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Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals.

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Section: Advantages Of Whole-cell Biocatalysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Whole-cell biocatalysts by design

2017

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“…These and other advantages and advances of whole cell biocatalysis are highlighted in this review. To keep a reasonable scope, we focus mostly on publications since 2012 and do not consider synthetic biology approaches utilizing entire natural or artificially introduced reaction pathways, which have been recently summarized [8][9][10]. The dynamic field of immobilization of whole cell catalysts will only be considered in context of particularly remarkable or novel techniques and reactor concepts, as immobilization of Escherichia coli whole cell has been nicely reviewed by Zajkoska et al recently [11].…”

Section: Introductionmentioning

confidence: 99%