The Microbial Community and Functional Potential in the Midland Basin Reveal a Community Dominated by Both Thiosulfate and Sulfate-Reducing Microorganisms

Tinker, Kara; Lipus, Daniel; Gardiner, James; Stuckman, Mengling; Gulliver, Djuna

doi:10.1128/spectrum.00049-22

Cited by 9 publications

(20 citation statements)

References 94 publications

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“…It can grow at pH ranging from 5.5-8.0, optimum temperature of 37 °C and optimum salinity of 12% (Ollivier et al, 1991). Under anaerobic conditions, Desulfohalobium retbaense can utilize H 2 as an electron donor and sulfate as electron acceptor producing H 2 S, for growth (Spring et al, 2010;Tinker et al, 2022). The organism was grown in the DSM 499 high salinity growth medium with some modifications: Base medium was: 1 g/L NH 4 Cl, 0.3 g/L K 2 HPO 4 , 0.3 g/L KH 2 PO 4 , 20 g/L MgCl 2 •6H 2 O, 100 g/L NaCl, 2.7 g/L CaCl 2 •2H 2 O, 4 g/L KCl, 3 g/L Na 2 SO 4 , 1 mL/L trace element SL-10, 0.5 mL/L Na-resazurin solution (0.1% w/v), 0.3 g/L Na 2 S•9 H 2 O.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Pore-scale study of microbial hydrogen consumption and wettability alteration during underground hydrogen storage

et al. 2023

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Hydrogen can be a renewable energy carrier and is suggested to store renewable energy and mitigate carbon dioxide emissions. Subsurface storage of hydrogen in salt caverns, deep saline formations, and depleted oil/gas reservoirs would help to overcome imbalances between supply and demand of renewable energy. Hydrogen, however, is one of the most important electron donors for many subsurface microbial processes, including methanogenesis, sulfate reduction, and acetogenesis. These processes cause hydrogen loss and changes of reservoir properties during geological hydrogen storage operations. Here, we report the results of a typical halophilic sulfate-reducing bacterium growing in a microfluidic pore network saturated with hydrogen gas at 35 bar and 37°C. Test duration is 9 days. We observed a significant loss of H2 from microbial consumption after 2 days following injection into a microfluidic device. The consumption rate decreased over time as the microbial activity declined in the pore network. The consumption rate is influenced profoundly by the surface area of H2 bubbles and microbial activity. Microbial growth in the silicon pore network was observed to change the surface wettability from a water-wet to a neutral-wet state. Due to the coupling effect of H2 consumption by microbes and wettability alteration, the number of disconnected H2 bubbles in the pore network increased sharply over time. These results may have significant implications for hydrogen recovery and gas injectivity. First, pore-scale experimental results reveal the impacts of subsurface microbial growth on H2 in storage, which are useful to estimate rapidly the risk of microbial growth during subsurface H2 storage. Second, microvisual experiments provide critical observations of bubble-liquid interfacial area and reaction rate that are essential to the modeling that is needed to make long-term predictions. Third, results help us to improve the selection criteria for future storage sites.

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Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Pore-scale study of microbial hydrogen consumption and wettability alteration during underground hydrogen storage

et al. 2023

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“…We used sediment and rock samples from a recent drilling campaign in the Hartoušov Mofette Field (HMF) the Eger Rift, West Bohemia, Czech Republic. Detailed geological, geochemical and microbial assessments of sediments from this environment are currently available elsewhere (Lipus et al, 2022). Most important findings from this baseline data are also reported below (e.g., Figure 1D) providing the foundation for the incubation experiments performed in this study.…”

Section: Introductionmentioning

confidence: 79%

“…The details of drilling and subsampling procedures can be found in Lipus et al (2022). Briefly, we reached a maximum depth of 238 m and recovered 163.15 m of core material (82.3% recovery rate).…”

Section: Site Description and Samplingmentioning

confidence: 99%

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Enrichment of rare methanogenic Archaea shows their important ecological role in natural high-CO2 terrestrial subsurface environments

et al. 2023

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IntroductionLong-term stability of underground CO2 storage is partially affected by microbial activity but our knowledge of these effects is limited, mainly due to a lack of sites. A consistently high flux of mantle-derived CO2 makes the Eger Rift in the Czech Republic a natural analogue to underground CO2 storage. The Eger Rift is a seismically active region and H2 is produced abiotically during earthquakes, providing energy to indigenous microbial communities.MethodsTo investigate the response of a microbial ecosystem to high levels of CO2 and H2, we enriched microorganisms from samples from a 239.5 m long drill core from the Eger Rift. Microbial abundance, diversity and community structure were assessed using qPCR and 16S rRNA gene sequencing. Enrichment cultures were set up with minimal mineral media and H2/CO2 headspace to simulate a seismically active period with elevated H2.Results and discussionMethane headspace concentrations in the enrichments indicated that active methanogens were almost exclusively restricted to enrichment cultures from Miocene lacustrine deposits (50–60 m), for which we observed the most significant growth. Taxonomic assessment showed microbial communities in these enrichments to be less diverse than those with little or no growth. Active enrichments were especially abundant in methanogens of the taxa Methanobacterium and Methanosphaerula. Concurrent to the emergence of methanogenic archaea, we also observed sulfate reducers with the metabolic ability to utilize H2 and CO2, specifically the genus Desulfosporosinus, which were able to outcompete methanogens in several enrichments. Low microbial abundance and a diverse non-CO2 driven microbial community, similar to that in drill core samples, also reflect the inactivity in these cultures. Significant growth of sulfate reducing and methanogenic microbial taxa, which make up only a small fraction of the total microbial community, emphasize the need to account for rare biosphere taxa when assessing the metabolic potential of microbial subsurface populations. The observation that CO2 and H2-utilizing microorganisms could only be enriched from a narrow depth interval suggests that factors such as sediment heterogeneity may also be important. This study provides new insight on subsurface microbes under the influence of high CO2 concentrations, similar to those found in CCS sites.

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“…Most members of the genus Marinobacter have been isolated from saline environments, such as sea water and sediment, tidal flats, saline soil, solar salterns and salt lakes [6][7][8][9][10][11][12] but they have also been isolated from sites as diverse as associated with diatoms [13], dinoflagellates [14,15] or sponges [16], from mangrove sediments [17], hydrothermal sediment [18], Antarctic environments [19][20][21][22] or from wine-barrel-decalcification wastewater [23]. Recently, 16S rRNA gene sequences related to this genus have also been reported in culture-independent studies in different habitats, such as an oil and gas reservoir [24], salt mines [25], associated with algae [26] or at Rio Tinto (Spain) [27].…”

Section: Introductionmentioning

confidence: 99%

Marinobacter iranensis sp. nov., a slightly halophilic bacterium from a hypersaline lake

Rafieyan,

Amoozegar,

Makzum

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel halophilic bacterium, strain 71-iT, was isolated from Inche-Broun hypersaline lake in Golestan province, in the north of Iran. It was a Gram-stain-negative, non-endospore forming, rod-shaped bacterium. It grew at 4–40 °C (optimum 30 °C), pH 6.0–11.0 (optimum pH 7.5) and with 0.5–15 % (w/v) NaCl [optimum 3 % (w/v) NaCl]. The results of phylogenetic analyses based on the 16S rRNA gene sequence comparison indicated its affiliation to the genus Marinobacter and the low percentage of identity with the most closely related species (97.5 %), indicated its placement as a novel species within this genus. Digital DNA–DNA hybridization (dDDH) values and average nucleotide identity (ANI) analyses of this strain against closely related species confirmed its condition of novel taxon. On the other hand, the percentage of the average amino acid identity (AAI) affiliated strain 71-iT within the genus Marinobacter . The DNA G+C content of this isolate was 57.7 mol%. The major fatty acids were C16 : 0 and C16 : 1ω7c and/or C16 : 1 ω6c. Ubiquinone-9 was the major isoprenoid quinone and diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were the main polar lipids of this strain. On the basis of the phylogenomic and phenotypic (including chemotaxonomic) features, we propose strain 71-iT (= IBRC M 11023T = CECT 30160T = LMG 29252T) as the type strain of a novel species within the genus Marinobacter , with the name Marinobacter iranensis sp. nov. Genomic detections of this strain in various metagenomic databases indicate that it is a relatively abundant species in environments with low salinities (approximately 5 % salinity), but not in hypersaline habitats with high salt concentrations.

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The Microbial Community and Functional Potential in the Midland Basin Reveal a Community Dominated by Both Thiosulfate and Sulfate-Reducing Microorganisms

Cited by 9 publications

References 94 publications

Pore-scale study of microbial hydrogen consumption and wettability alteration during underground hydrogen storage

Pore-scale study of microbial hydrogen consumption and wettability alteration during underground hydrogen storage

Enrichment of rare methanogenic Archaea shows their important ecological role in natural high-CO2 terrestrial subsurface environments

Marinobacter iranensis sp. nov., a slightly halophilic bacterium from a hypersaline lake

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