The Microbial Community Composition of a Nitrifying-Denitrifying Activated Sludge from an Industrial Sewage Treatment Plant Analyzed by the Full-Cycle rRNA Approach

Juretschko, Stefan; Loy, Alexander; Lehner, Angelika; Wagner, Michael

doi:10.1078/0723-2020-00093

Cited by 344 publications

(247 citation statements)

References 44 publications

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“…The species richness calculated is 411. It should, however, be regarded as a minimum richness value because, as analysed elsewhere (Juretschko et al, 2002), not all OTUs with more than 97.0 % similarity (recently a more stringent standard of 99 % similarity has been proposed; Stackebrandt & Ebers, 2006) belong to one species and the primers used in PCR amplification of the 16S rRNA gene did not cover all bacteria. To cover most of the biodiversity in the Skagen WWTP at least 400-500 clones would be required.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is not possible to make direct comparisons of the biodiversity found in this study with that of others. Only a single study on a full-scale industrial WWTP with nitrificationdenitrification has been conducted (Juretschko et al, 2002). The species richness is much larger for Skagen (411) than was estimated by Juretschko et al (2002) for an industrial N-removal WWTP (89).…”

Section: Discussionmentioning

confidence: 99%

“…LGCmix [equimolar concentrations of LGC354A, LGC354B and LGC354C (Meier et al, 1999)], TM7905 (Hugenholtz et al, 2001), Ntspa1413 (Maixner et al, 2006), HoAc1402 (Juretschko et al, 2002), CFX1223 and GNSB-941 (Bjornsson et al, 2002), Actino-221, Actino-651 (Kong et al, 2005), and PAOmix [equimolar concentrations of PAO651, PAO462 and PAO846 (Crocetti et al, 2000)], GB_G1 (GAOQ989) (Crocetti et al, 2002) and GB_G2, GB_1&2, GB_3, GB_4, GB_5, GB_7 (Kong et al, 2002), GB_6 [Gam1019 (Nielsen et al, 1999)], Cluster 6a-192 (Adamczyk et al, 2003), ZRA23a (Rossello-Mora et al, 1995), Azo644 (Hess et al, 1997), Thau-646 (Lajoie et al, 2000), Aqs997 (Thomsen et al, 2004), Sap309 (Schauer & Hahn, 2005), CF319a and CF319b (Manz et al, 1996) were used for FISH. All the probes, except the EUBmix, which was labelled with FLUOS, were labelled with either Cy3 or Cy5 (only for specificity check).…”

Section: Methodsmentioning

confidence: 99%

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Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

et al. 2007

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The structure and function of the microbial community in a full-scale enhanced biological phosphorus removal wastewater treatment plant (WWTP; Skagen) were investigated using the full-cycle rRNA approach, combined with ecophysiological studies. A total of 87 16S rRNA gene sequences were retrieved, and 78 operational taxonomic units were identified. Novel oligonucleotide probes were designed, and quantitative fluorescence in situ hybridization revealed that six hitherto undescribed probe-defined groups within the phylum Bacteroidetes (two groups), and classes Betaproteobacteria (two groups) and Gammaproteobacteria (two groups), were relatively abundant (.1 % of total biovolume) in the Skagen WWTP and 10 other full-scale WWTPs with biological P removal. The most abundant was a group of rod-shaped Bacteroidetes attached to filamentous bacteria, which is distantly related to the genus Haliscomenobacter of the family Saprospiraceae, and comprised 9-19 % of the bacterial biovolume in all the WWTPs investigated. The other five probe-defined groups were found in all WWTPs, but they were less abundant (1-6 %). Two groups had a glycogen-accumulating phenotype and one Dechloromonas-related group had a polyphosphate-accumulating phenotype, and they were potentially all involved in denitrification. In total, about 81 % of all bacteria hybridizing with the general eubacterial probe were detected in the Skagen WWTP by using clone-or group-specific probes, indicating that most members of the microbial community had been identified. INTRODUCTIONAn increasing number of wastewater treatment plants (WWTPs) are designed, or have been upgraded, to remove C, N and P by using microbial activity in the process known as enhanced biological phosphorus removal (EBPR). This process is popular mainly because it is more environmentally friendly, due to reduced use of chemicals and low sludge production, compared to P removal by chemical precipitation (Seviour et al., 2003). The general understanding of design and operation of activated-sludge WWTPs has increased significantly in the last two decades, but occasionally EBPR WWTPs still suffer from suboptimal operation and breakdown of the P-removal process. These problems are believed to be due to poor understanding of the structure of the microbial community in EBPR WWTPs and insufficient knowledge of the ecophysiology of the key microbial populations.Our understanding of the microbiology of EBPR WWTPs is to a large extent obtained from culture-independent studies using enriched cultures in well-controlled laboratory-scale reactors by using the full-cycle rRNA approach (Amann, 1995), as reviewed in detail by Mino et al. (1998) and Seviour et al. (2003). Some potentially important micro-organisms involved in the EBPR process have been identified in such laboratory-scale reactors, but only a few of them have been shown to be important in full-scale WWTPs. These are all uncultured organisms and can thus only be detected and quantified by using molecular methods such as fluorescence in situ hybrid...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

et al. 2007

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“…The resulting pellet was resuspended in 0.5 ml of 50% ethanol-phosphate-buffered saline (v/v) and this suspension was stored at À20 1C until use. The Cy3-labeled oligonucleotide probes HoAc1402 and EUB338-mix with reported group specificity for the Acidobacteria and members of the domain Bacteria (Daims et al, 1999;Juretschko et al, 2002) were used in this study. Hybridization was carried out on gelatin-coated (0.1%, w/v) and dried Teflon-laminated slides (MAGV, Rabenau, Germany) with eight wells for independent positioning of the samples.…”

Section: Fishmentioning

confidence: 99%

Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

et al. 2008

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Fluorescence in situ hybridization (FISH) was applied to estimate the population size of the poorly characterized phylum Acidobacteria in acidic peat sampled from nine different Sphagnumdominated wetlands of Northern Russia. The cell numbers of these bacteria in oxic peat layers ranged from 0.4 Â 10 6 to 1.3 Â 10 7 cells per g of wet peat, comprising up to 4% of total bacterial cells. Substrate-induced growth of acidobacteria was observed after amendment of peat samples with glucose, pectin, xylan, starch, ethanol and methanol, while weak or no response was obtained for acetate, pyruvate, mannitol and cellobiose. Using low-nutrient media and FISH-mediated monitoring of the isolation procedure, we succeeded in obtaining nine strains of acidobacteria in pure cultures. These strains belonged to subdivisions 1 and 3 of the Acidobacteria and represented strictly aerobic, heterotrophic organisms. Except for methanol, the substrate utilization patterns of these isolates matched the results obtained in our substrate-amendment experiments with native peat. All strains were also capable of utilizing galacturonic acid, a characteristic component of the cell wall in Sphagnum spp, which is released during moss decomposition. Most isolates from subdivision 1 were truly acidophilic organisms with the growth optimum at pH 3.5-4.5, while the isolates from subdivision 3 grew optimally at pH 5.5-6.5. Another important phenotypic trait of novel strains was their capability of active growth at low temperatures. Both acidophily and low-temperature growth are consistent with the occurrence of acidobacteria in cold and acidic northern wetlands. The ISME Journal (

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“…anaerobic system envelopes (Oxoid) for 4 weeks at 20 u C. Microbial biofilms that developed on these plates were screened by hybridization with Cy3-labelled probe HoAc1402, with reported group specificity for the phylum Acidobacteria (Juretschko et al, 2002). This probe hybridized to relatively large, rod-shaped cells that were present on some of the plates.…”

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confidence: 99%

Paludibaculum fermentans gen. nov., sp. nov., a facultative anaerobe capable of dissimilatory iron reduction from subdivision 3 of the Acidobacteria

Kulichevskaya

Suzina

Rijpstra

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Paludibaculum fermentans gen. nov., sp. nov., a facultative anaerobe capable of dissimilatory iron reduction from subdivision 3 of the Acidobacteria A facultatively anaerobic, non-pigmented, non-spore-forming bacterium was isolated from a littoral wetland of a boreal lake located on Valaam Island, northern Russia, and designated strain P105 T . Cells of this isolate were Gram-negative, non-motile rods coated by S-layers with p2 lattice symmetry. Sugars were the preferred growth substrates. Under anoxic conditions, strain P105 T was capable of fermentation and dissimilatory Fe(III) reduction. End products of fermentation were acetate, propionate and H 2 . Strain P105 T was a mildly acidophilic, mesophilic organism, capable of growth at pH 4.0-7.2 (optimum pH 5.5-6.0) and at 4-35 6C (optimum at 20-28 6C). The major fatty acids were iso-C 15 : 0 and C 16 : 1 v7c; the cells also contained significant amounts of 13,16-dimethyl octacosanedioic acid (isodiabolic acid). The major polar lipids were phosphocholine and phosphoethanolamine; the quinone was MK-8. The G+C content of the DNA was 60.5 mol%. 16S rRNA gene sequence analysis showed that strain P105 T belongs to subdivision 3 of the Acidobacteria and is only distantly related (90 % sequence similarity) to the only currently characterized member of this subdivision, Bryobacter aggregatus. The novel isolate differs from Bryobacter aggregatus in its cell morphology and ability to grow under anoxic conditions and in the presence of iron-and nitrate-reducing capabilities as well as quinone and polar lipid compositions. These differences suggest that strain P105 T represents a novel genus and species, for which the name Paludibaculum fermentans gen. nov., sp. nov., is proposed. The type strain of Paludibaculum fermentans is P105 T (5DSM 26340 T 5VKM B-2878 T ).Members of the phylum Acidobacteria represent one of the dominant bacterial groups in northern acidic wetlands (Dedysh et al., 2006;Dedysh, 2011;Pankratov et al., 2011;Lin et al., 2012;Serkebaeva et al., 2013). As revealed by molecular surveys, these bacteria inhabit both oxic and anoxic peat layers and mostly belong to subdivisions 1 and 3 of the Acidobacteria (Lin et al., 2012;Serkebaeva et al., 2013). The proportion of 16S rRNA gene sequences that can be assigned to taxonomically characterized members of the Acidobacteria is significantly higher in the surface layer than in the subsurface peat, indicating a major lack of knowledge about acidobacteria that thrive in anoxic peat (Serkebaeva et al., 2013). Indeed, with the exception of Telmatobacter bradus and Acidobacterium capsulatum (Pankratov et al., 2012), all currently characterized members of subdivisions 1 and 3 are strict aerobes. This study, therefore, aimed at isolation and characterization of those acidobacteria that inhabit anoxic peat layers of northern wetlands.Samples were collected from the anoxic peat layer (depth of 40-50 cm) of the littoral wetland of a boreal lake located on Valaam Island (61 u 229 N 30 u 569 E) in Lake Ladoga, Republic of Kare...

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The Microbial Community Composition of a Nitrifying-Denitrifying Activated Sludge from an Industrial Sewage Treatment Plant Analyzed by the Full-Cycle rRNA Approach

Cited by 344 publications

References 44 publications

Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

Paludibaculum fermentans gen. nov., sp. nov., a facultative anaerobe capable of dissimilatory iron reduction from subdivision 3 of the Acidobacteria

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