The microbial production of poly(hydroxyalkanoates) from tallow

Cromwick, Anne-Marie; Foglia, Thomas A.; Lenz, Robert W.

doi:10.1007/s002530050845

Cited by 130 publications

(69 citation statements)

References 11 publications

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“…CMG634. Pseudomonas oleovorans Gpo1 and Pseudomonas putida have been reported to produce mcl-PHA (Cromwick et al, 1996) and have been used as reference strains (Kraak et al, 1997). Results showed that with the exception of Alcaligenes sp.…”

Section: Resultsmentioning

confidence: 75%

Characterization of biopolymer produced by Pseudomonas sp. CMG607w of marine origin

Jamil

Ahmed

Edwards

2007

J. Gen. Appl. Microbiol.

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Biopolymer (polyhydroxyalkanoate, PHA) was extracted and purified from CMG607w bacterial strain isolated from sediment of Layari River outfall to the Arabian Sea. Synthesis of PHA was substrate depended in CMG607w. In the presence of sodium gluconate mcl-PHA was synthesized at the rate of 42% cell dry mass. Under highly enriched conditions, co-production of polysaccharide and blends of poly(3-hydroxybutyrate) (PHB)/PHA was observed. PCR base strategy was used to amplify PHA polymerase genes from chromosomal DNA. Conserved sequences were observed in polymerase C1 and C2. A portion of polymerase C1 and C2 genes of the pha operon was cloned and sequenced. In CMG607w PHA biosynthsis the operon has phaC1 and C2 (polymerase 1 and polymerase 2) genes. pha polymerase C1/C2 genes of CMG607w have 98% homology to Pseudomonas aeruginosa PAO1 (AE004919). Gene sequences were submitted to GenBank under accession numbers EF028075, AY596788 and AY596790.

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Section: Resultsmentioning

confidence: 75%

Characterization of biopolymer produced by Pseudomonas sp. CMG607w of marine origin

Jamil

Ahmed

Edwards

2007

J. Gen. Appl. Microbiol.

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“…For chromatography-based methods, the analysis of PHA polymer is similar to that for intracellular PHA, requiring depolymerization of the polymer, usually combined with chemical derivatization before it can be analyzed [43,130,132], which meant that chromatography methods cannot analyze PHA as an intact polymer. NMR on the other hand, could study the chemical makeup of an intact PHA polymer and differentiate between PHA blends and PHA copolymer though providing details on the topology and functional group in molecules [150,153,154,168]. Typically, two types of NMR techniques are available and they are C-NMR are usually applied in combination to provide a more comprehensive analysis of the polymer.…”

Section: Monomeric Composition and Distributionmentioning

confidence: 99%

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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“…The polymer was isolated from lyophilized cells by Soxhlet extraction in chloroform according to the method of Cromwick et al [11]. Then the polymers were precipitated by slowly adding methanol.…”

Section: Polymer Extraction and Purificationmentioning

confidence: 99%

Investigation of the effects of the types of food waste utilized as carbon source on the molecular weight distributions and thermal properties of polyhydroxybutyrate produced by two strains of microorganisms

Wong

Cheung

et al. 2004

E-Polymers

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Abstract:The average molecular weight and molecular weight distribution of polyhydroxybutyrate can be affected by different bacterial strains, different cultivation time and also different carbon sources. Food waste was proven to have a high potential in the induction of biosynthesis of polyhydroxybutyrate with different production yields and physical properties. A specific culture of Alcaligenes latus DSM 1124 and Staphylococcus epidermidis, which was isolated from sesame oil, were selected to ferment several types of food wastes as nutrients, including malt waste, soy waste, confectionery waste, milk waste, vinegar waste and sesame oil, into polyhydroxybutyrate in this study. Average molecular weight and molecular weight distribution of polyhydroxybutyrate were measured by gel permeation chromatography. By comparing the results with those obtained using sucrose as a carbon source, the average molecular weight of polyhydroxybutyrate produced from food wastes was increased for Alcaligenes latus; however, it was decreased for S. epidermidis. Thermal analyses of the biopolymer produced by S. epidermidis indicated that the melting point of the polymer produced from sesame oil as carbon source was 188°C, the highest temperature among those polymers produced by using ice cream, malt, and soya wastes.

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The microbial production of poly(hydroxyalkanoates) from tallow

Cited by 130 publications

References 11 publications

Characterization of biopolymer produced by Pseudomonas sp. CMG607w of marine origin

Characterization of biopolymer produced by Pseudomonas sp. CMG607w of marine origin

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Investigation of the effects of the types of food waste utilized as carbon source on the molecular weight distributions and thermal properties of polyhydroxybutyrate produced by two strains of microorganisms

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